巴西东北部地区一家山羊养殖场的流产风波是由非典型弓形虫基因型 #13 引起的。

Vinícius Longo Ribeiro Vilela , Thais Ferreira Feitosa , Sara Vilar Dantas Simões , Rinaldo Aparecido Mota , Frank Katzer , Paul M. Bartley
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引用次数: 0

摘要

本研究的目的是了解巴西东北部帕拉伊巴州一家山羊养殖场爆发的弓形虫诱发流产疫情的特点。羊群中有 10 头母羊,其中 7 头流产,1 头产下双胞胎(1 头死胎,另 1 头体质虚弱、发育不良)。所有母鹿的血清样本都通过间接荧光抗体检测(IFAT)进行了分析。两头母羊的初乳和胎盘样本,以及四个胎儿的肺、心、脑和脐带样本,都通过巢式 ITS1 PCR 进行了筛查。然后用多重巢式 PCR-RFLP 对阳性样本进行分析。经 IFAT 检测,所有 10 头母牛的抗淋病双球菌 IgG 都呈阳性(滴定度从 1:4096 到 1:65,536)。ITS1 PCR筛查在胎盘(2/2)、初乳(2/2)、脐带(2/4)、肺(1/4)、心脏(1/4)和大脑(1/4)中发现了淋病双球菌DNA。四个样本产生了完整的 RFLP 基因分型结果,确定了一个基因型,即 ToxoDB #13。总之,我们在一个山羊群中发现了由淋病双球菌引起的高流产率,凸显了 13 号基因型的致病性,它是巴西淋病双球菌最普遍的基因型之一。
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An abortion storm in a goat farm in the Northeast Region of Brazil was caused by the atypical Toxoplasma gondii genotype #13

The objective of this study was to characterise a Toxoplasma gondii-induced abortion outbreak on a goat farm in the State of Paraíba, Northeast Region of Brazil. From a herd of 10 does, seven experienced abortions and one gave birth to twins (one stillborn and the other weak and underdeveloped). Serum samples from all of the does were analysed by indirect fluorescent antibody test (IFAT). Samples of colostrum and placenta from two does, along with lung, heart, brain and umbilical cord samples from four of the foetuses, were screened by nested ITS1 PCR specific for T. gondii. The positive samples were then analysed by multiplex nested PCR-RFLP. All ten does tested positive by IFAT for anti-T. gondii IgG (titrations ranging from 1:4096 to 1:65,536). The ITS1 PCR screening revealed T. gondii DNA in the placenta (2/2), colostrum (2/2), umbilical cord (2/4), lung (1/4), heart (1/4), and brain (1/4). Four samples produced complete RFLP genotyping results, identifying a single genotype, ToxoDB #13. In conclusion, we demonstrated a high rate of abortion caused by T. gondii in a goat herd, highlighting the pathogenicity of genotype #13, one of the most prevalent genotypes of T. gondii in Brazil.

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