Marianna Justin, Jessica Jeyanthi James Antony, Eldred Anak Embu, S. Subramaniam
{"title":"优化营养培养基中不同的叶黄素和细胞分裂素组合,以建立榕树(Ficus carica L. cv Siyah Orak)最佳离体多倍体小植株","authors":"Marianna Justin, Jessica Jeyanthi James Antony, Eldred Anak Embu, S. Subramaniam","doi":"10.55230/mabjournal.v52i5.cp19","DOIUrl":null,"url":null,"abstract":"Ficus carica Linnaeus is a flowering plant under the Moraceae family, usually propagated conventionally from cuttings due to the seeds being non-viable. However, this method is prone to diseases, and pests, time-consuming and space-intensive. Therefore, other methods are needed to overcome these issues. This study was conducted to induce callus and multiple shoots via plant tissue culture techniques enabling mass production of fig plants. Initially, leaf segments of Ficus carica L. cv Siyah Orak were cultured on different MS media strengths (¼, ½, ¾,1 MS) to induce callus. The highest callus means weight was observed on explant cultured in ¾ MS media (875±0.036). Callus was proliferated by subculturing explant into ¾ MS media supplemented with different concentrations of TDZ (0, 0.5, 1.0, 1.5, 2.0, 2.5, 3.0 mg/L). MS media (3/4) supplemented with 2.0 mg/L TDZ (920±0.03) shows the best result for callus proliferation. Callus induction using transverse and longitudinal thin cell layers from nodal segments cultured on different MS media strengths (¼, ½, ¾,1 MS) shows ¼ MS as the optimum media for both tTCL (100±0) and lTCL (96.7±0.15). Friable callus (%) was observed the highest on ½ MS (63.33±0.55) and ¼ MS (76.67±0.50) media for both tTCL and lTCL, respectively. As for the number of leaves produced, both tTCL (0.83±0.0.28) and lTCL (1.00±0.33) explant showed the best results in ¼ MS media. Apical buds produced the highest mean for both the number of leaves and length of the shoot on 1MS media supplemented with 2.0 mg/L BAP (3.5±0.20, 13.73±0.66), respectively. For root formation (%) and number of roots, both show the best results in media supplemented with 2.5 mg/L IAA (10±0.31, 0.83±0.50). It can be concluded that the best shoot growth performance was observed from apical bud cultured on 1MS media supplemented with 2.0 mg/L BAP+ 2.5 mg/L IAA.","PeriodicalId":18160,"journal":{"name":"Malaysian applied biology","volume":"95 4","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2023-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Optimization of Different Auxin and Cytokinin Combination in Nutrient Medium for Establishment of Optimal in vitro Multiple Plantlet in Ficus carica L. cv Siyah Orak\",\"authors\":\"Marianna Justin, Jessica Jeyanthi James Antony, Eldred Anak Embu, S. Subramaniam\",\"doi\":\"10.55230/mabjournal.v52i5.cp19\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Ficus carica Linnaeus is a flowering plant under the Moraceae family, usually propagated conventionally from cuttings due to the seeds being non-viable. However, this method is prone to diseases, and pests, time-consuming and space-intensive. Therefore, other methods are needed to overcome these issues. This study was conducted to induce callus and multiple shoots via plant tissue culture techniques enabling mass production of fig plants. Initially, leaf segments of Ficus carica L. cv Siyah Orak were cultured on different MS media strengths (¼, ½, ¾,1 MS) to induce callus. The highest callus means weight was observed on explant cultured in ¾ MS media (875±0.036). Callus was proliferated by subculturing explant into ¾ MS media supplemented with different concentrations of TDZ (0, 0.5, 1.0, 1.5, 2.0, 2.5, 3.0 mg/L). MS media (3/4) supplemented with 2.0 mg/L TDZ (920±0.03) shows the best result for callus proliferation. Callus induction using transverse and longitudinal thin cell layers from nodal segments cultured on different MS media strengths (¼, ½, ¾,1 MS) shows ¼ MS as the optimum media for both tTCL (100±0) and lTCL (96.7±0.15). Friable callus (%) was observed the highest on ½ MS (63.33±0.55) and ¼ MS (76.67±0.50) media for both tTCL and lTCL, respectively. As for the number of leaves produced, both tTCL (0.83±0.0.28) and lTCL (1.00±0.33) explant showed the best results in ¼ MS media. Apical buds produced the highest mean for both the number of leaves and length of the shoot on 1MS media supplemented with 2.0 mg/L BAP (3.5±0.20, 13.73±0.66), respectively. For root formation (%) and number of roots, both show the best results in media supplemented with 2.5 mg/L IAA (10±0.31, 0.83±0.50). 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引用次数: 0
摘要
榕树(Ficus carica Linnaeus)是桑科榕属的一种开花植物,由于种子无法存活,通常采用传统的扦插繁殖方法。然而,这种方法容易受到病虫害的影响,而且耗时耗地。因此,需要其他方法来克服这些问题。本研究通过植物组织培养技术诱导胼胝体和多枝芽,从而实现无花果植株的大规模生产。最初,将无花果(Ficus carica L. cv Siyah Orak)的叶片培养在不同强度的 MS 培养基(¼、½、¾、1 MS)上以诱导胼胝体。在 ¾ MS 培养基上培养的外植体胼胝体平均重量最高(875±0.036)。将外植体移入添加了不同浓度 TDZ(0、0.5、1.0、1.5、2.0、2.5、3.0 毫克/升)的 ¾ MS 培养基中进行亚培养,可使胼胝体增殖。添加 2.0 mg/L TDZ 的 MS 培养基(3/4)(920±0.03)对胼胝体增殖的效果最好。利用在不同强度的 MS 培养基(¼、½、¾、1 MS)上培养的节段横向和纵向薄细胞层诱导胼胝体的结果表明,¼ MS 是 tTCL(100±0)和 lTCL(96.7±0.15)的最佳培养基。对于 tTCL 和 lTCL,在 ½ MS(63.33±0.55)和 ¼ MS(76.67±0.50)培养基上观察到的易碎胼胝体(%)最高。至于产生的叶片数,tTCL(0.83±0.0.28)和 lTCL(1.00±0.33)外植体在 ¼ MS 培养基中表现最好。在添加了 2.0 mg/L BAP 的 1MS 培养基上,顶芽产生的叶片数和芽体长度的平均值最高,分别为(3.5±0.20,13.73±0.66)。在生根率(%)和根数方面,添加 2.5 毫克/升 IAA 的培养基效果最好(10±0.31,0.83±0.50)。因此,在添加了 2.0 毫克/升 BAP+ 2.5 毫克/升 IAA 的 1MS 培养基上培养出的顶芽生长表现最好。
Optimization of Different Auxin and Cytokinin Combination in Nutrient Medium for Establishment of Optimal in vitro Multiple Plantlet in Ficus carica L. cv Siyah Orak
Ficus carica Linnaeus is a flowering plant under the Moraceae family, usually propagated conventionally from cuttings due to the seeds being non-viable. However, this method is prone to diseases, and pests, time-consuming and space-intensive. Therefore, other methods are needed to overcome these issues. This study was conducted to induce callus and multiple shoots via plant tissue culture techniques enabling mass production of fig plants. Initially, leaf segments of Ficus carica L. cv Siyah Orak were cultured on different MS media strengths (¼, ½, ¾,1 MS) to induce callus. The highest callus means weight was observed on explant cultured in ¾ MS media (875±0.036). Callus was proliferated by subculturing explant into ¾ MS media supplemented with different concentrations of TDZ (0, 0.5, 1.0, 1.5, 2.0, 2.5, 3.0 mg/L). MS media (3/4) supplemented with 2.0 mg/L TDZ (920±0.03) shows the best result for callus proliferation. Callus induction using transverse and longitudinal thin cell layers from nodal segments cultured on different MS media strengths (¼, ½, ¾,1 MS) shows ¼ MS as the optimum media for both tTCL (100±0) and lTCL (96.7±0.15). Friable callus (%) was observed the highest on ½ MS (63.33±0.55) and ¼ MS (76.67±0.50) media for both tTCL and lTCL, respectively. As for the number of leaves produced, both tTCL (0.83±0.0.28) and lTCL (1.00±0.33) explant showed the best results in ¼ MS media. Apical buds produced the highest mean for both the number of leaves and length of the shoot on 1MS media supplemented with 2.0 mg/L BAP (3.5±0.20, 13.73±0.66), respectively. For root formation (%) and number of roots, both show the best results in media supplemented with 2.5 mg/L IAA (10±0.31, 0.83±0.50). It can be concluded that the best shoot growth performance was observed from apical bud cultured on 1MS media supplemented with 2.0 mg/L BAP+ 2.5 mg/L IAA.