O. A. Shashkova, I. V. Smirnov, A. A. Pinevich, K. O. Avrov, L. A. Terekhina, I. S. Malakhov, A. Yu. Stolbovaya, I. V. Gryazeva, N. L. Vartanyan, I. Yu. Krutetskaya, D. O. Antuganov, S. V. Shatik, M. P. Samoilovich
{"title":"以重组细胞为工具评估针对内切蛋白（CD105）的放射性标记抗体的特异性活性","authors":"O. A. Shashkova, I. V. Smirnov, A. A. Pinevich, K. O. Avrov, L. A. Terekhina, I. S. Malakhov, A. Yu. Stolbovaya, I. V. Gryazeva, N. L. Vartanyan, I. Yu. Krutetskaya, D. O. Antuganov, S. V. Shatik, M. P. Samoilovich","doi":"10.1134/S0003683823070049","DOIUrl":null,"url":null,"abstract":"<div><p>One of the most important characteristics of radiolabeled antibodies and their derivatives is the size of the immunoreactive fraction. Measuring this parameter requires a high density of target molecules, which is rarely achievable with tumor cells. The solution to the problem of radioimmunoconjugate testing was the creation of recombinant cells carrying human endoglin (CD105). The recipients of the endoglin gene (<i>ENG</i>) were rat C6 glioma cells, which are characterized by ease of cultivation and high transfection efficiency. The obtained C6-ENG cells carried 1.3 × 10<sup>6</sup> CD105 molecules on the membrane and were used to determine the immunoreactive fraction of <sup>68</sup>Ga and <sup>89</sup>Zr radiolabeled anti-CD105 monoclonal antibodies and their Fab-fragments. The creation of stable recombinant cell lines for in vitro testing the specific activity of radiolabeled antibodies and their derivatives seems promising for the development of new radiopharmaceuticals.</p></div>","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":null,"pages":null},"PeriodicalIF":1.0000,"publicationDate":"2023-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Recombinant Cells as a Tool for Evaluating the Specific Activity of Radiolabeled Antibodies against Endoglin (CD105)\",\"authors\":\"O. A. Shashkova, I. V. Smirnov, A. A. Pinevich, K. O. Avrov, L. A. Terekhina, I. S. Malakhov, A. Yu. Stolbovaya, I. V. Gryazeva, N. L. Vartanyan, I. Yu. Krutetskaya, D. O. Antuganov, S. V. Shatik, M. P. Samoilovich\",\"doi\":\"10.1134/S0003683823070049\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>One of the most important characteristics of radiolabeled antibodies and their derivatives is the size of the immunoreactive fraction. Measuring this parameter requires a high density of target molecules, which is rarely achievable with tumor cells. The solution to the problem of radioimmunoconjugate testing was the creation of recombinant cells carrying human endoglin (CD105). The recipients of the endoglin gene (<i>ENG</i>) were rat C6 glioma cells, which are characterized by ease of cultivation and high transfection efficiency. The obtained C6-ENG cells carried 1.3 × 10<sup>6</sup> CD105 molecules on the membrane and were used to determine the immunoreactive fraction of <sup>68</sup>Ga and <sup>89</sup>Zr radiolabeled anti-CD105 monoclonal antibodies and their Fab-fragments. The creation of stable recombinant cell lines for in vitro testing the specific activity of radiolabeled antibodies and their derivatives seems promising for the development of new radiopharmaceuticals.</p></div>\",\"PeriodicalId\":466,\"journal\":{\"name\":\"Applied Biochemistry and Microbiology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.0000,\"publicationDate\":\"2023-12-14\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Applied Biochemistry and Microbiology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://link.springer.com/article/10.1134/S0003683823070049\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Applied Biochemistry and Microbiology","FirstCategoryId":"99","ListUrlMain":"https://link.springer.com/article/10.1134/S0003683823070049","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
Recombinant Cells as a Tool for Evaluating the Specific Activity of Radiolabeled Antibodies against Endoglin (CD105)
One of the most important characteristics of radiolabeled antibodies and their derivatives is the size of the immunoreactive fraction. Measuring this parameter requires a high density of target molecules, which is rarely achievable with tumor cells. The solution to the problem of radioimmunoconjugate testing was the creation of recombinant cells carrying human endoglin (CD105). The recipients of the endoglin gene (ENG) were rat C6 glioma cells, which are characterized by ease of cultivation and high transfection efficiency. The obtained C6-ENG cells carried 1.3 × 106 CD105 molecules on the membrane and were used to determine the immunoreactive fraction of 68Ga and 89Zr radiolabeled anti-CD105 monoclonal antibodies and their Fab-fragments. The creation of stable recombinant cell lines for in vitro testing the specific activity of radiolabeled antibodies and their derivatives seems promising for the development of new radiopharmaceuticals.
期刊介绍：
Applied Biochemistry and Microbiology is an international peer reviewed journal that publishes original articles on biochemistry and microbiology that have or may have practical applications. The studies include: enzymes and mechanisms of enzymatic reactions, biosynthesis of low and high molecular physiologically active compounds; the studies of their structure and properties; biogenesis and pathways of their regulation; metabolism of producers of biologically active compounds, biocatalysis in organic synthesis, applied genetics of microorganisms, applied enzymology; protein and metabolic engineering, biochemical bases of phytoimmunity, applied aspects of biochemical and immunochemical analysis; biodegradation of xenobiotics; biosensors; biomedical research (without clinical studies). Along with experimental works, the journal publishes descriptions of novel research techniques and reviews on selected topics.
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