{"title":"在三轴磁场中运行的微流控芯片中的颗粒单向传输,用于颗粒浓缩和生物分析检测","authors":"Negar Sadeghidelouei, Roozbeh Abedini-Nassab","doi":"10.1007/s10404-023-02702-y","DOIUrl":null,"url":null,"abstract":"<div><p>The Controlled transport of tiny particles in a microfluidic environment has attracted the attention of numerous researchers in the field of lab-on-a-chip. In this work, for the first time, a fully operational microfluidic chip composed of asymmetric magnetic tracks that unidirectionally transport multiple magnetic particles synced with a general tri-axial magnetic field is proposed. In this innovative chip, the particle motion is analogous to the electron transport in electrical diodes, with similar controllability and automation levels not seen in other single-particle manipulation systems. The vertical bias component of the magnetic field by providing a repulsive force between the particles and preventing undesired cluster formation, makes the proposed chip even more similar to the electrical circuits. Additionally, the chip functions as a highly sensitive biosensor capable of detecting extremely low levels of DNA fragments using ligand-functionalized magnetic beads. The uniqueness of the proposed sensor lies in the introduction of a novel particle/analyte concentrator based on the proposed diodes, which enhances its detection sensitivity. This sensitivity is even further enhanced by a single-particle and pair detection image processing code. Furthermore, the background noise is reduced by eliminating the unwanted bead cluster formation commonly observed in previous works. 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引用次数: 0
摘要
微流体环境中微小颗粒的可控传输吸引了芯片实验室领域众多研究人员的关注。在这项研究中,我们首次提出了一种完全可操作的微流控芯片,它由非对称磁轨组成,可在一般三轴磁场中同步单向传输多个磁性粒子。在这一创新芯片中,粒子运动类似于电子二极管中的电子传输,具有其他单粒子操纵系统所不具备的类似可控性和自动化水平。磁场的垂直偏置分量可在粒子之间产生排斥力,防止形成不必要的粒子簇,从而使拟议的芯片与电路更加相似。此外,该芯片还可用作高灵敏度生物传感器,利用配体功能化磁珠检测极低水平的 DNA 片段。该传感器的独特之处在于采用了基于二极管的新型粒子/分析物浓缩器,从而提高了检测灵敏度。单粒子和成对检测图像处理代码进一步提高了灵敏度。此外,由于消除了以往工作中常见的不需要的微珠簇形成,从而降低了背景噪声。所提出的装置可作为单粒子分辨率的高通量单向传输系统,提供灵敏的生物检测,在生物医学中有着广泛的应用。
Unidirectional particle transport in microfluidic chips operating in a tri-axial magnetic field for particle concentration and bio-analyte detection
The Controlled transport of tiny particles in a microfluidic environment has attracted the attention of numerous researchers in the field of lab-on-a-chip. In this work, for the first time, a fully operational microfluidic chip composed of asymmetric magnetic tracks that unidirectionally transport multiple magnetic particles synced with a general tri-axial magnetic field is proposed. In this innovative chip, the particle motion is analogous to the electron transport in electrical diodes, with similar controllability and automation levels not seen in other single-particle manipulation systems. The vertical bias component of the magnetic field by providing a repulsive force between the particles and preventing undesired cluster formation, makes the proposed chip even more similar to the electrical circuits. Additionally, the chip functions as a highly sensitive biosensor capable of detecting extremely low levels of DNA fragments using ligand-functionalized magnetic beads. The uniqueness of the proposed sensor lies in the introduction of a novel particle/analyte concentrator based on the proposed diodes, which enhances its detection sensitivity. This sensitivity is even further enhanced by a single-particle and pair detection image processing code. Furthermore, the background noise is reduced by eliminating the unwanted bead cluster formation commonly observed in previous works. The proposed device serves as a high-throughput unidirectional transport system at the single-particle resolution, offering sensitive bio-detection with many applications in biomedicine.
期刊介绍:
Microfluidics and Nanofluidics is an international peer-reviewed journal that aims to publish papers in all aspects of microfluidics, nanofluidics and lab-on-a-chip science and technology. The objectives of the journal are to (1) provide an overview of the current state of the research and development in microfluidics, nanofluidics and lab-on-a-chip devices, (2) improve the fundamental understanding of microfluidic and nanofluidic phenomena, and (3) discuss applications of microfluidics, nanofluidics and lab-on-a-chip devices. Topics covered in this journal include:
1.000 Fundamental principles of micro- and nanoscale phenomena like,
flow, mass transport and reactions
3.000 Theoretical models and numerical simulation with experimental and/or analytical proof
4.000 Novel measurement & characterization technologies
5.000 Devices (actuators and sensors)
6.000 New unit-operations for dedicated microfluidic platforms
7.000 Lab-on-a-Chip applications
8.000 Microfabrication technologies and materials
Please note, Microfluidics and Nanofluidics does not publish manuscripts studying pure microscale heat transfer since there are many journals that cover this field of research (Journal of Heat Transfer, Journal of Heat and Mass Transfer, Journal of Heat and Fluid Flow, etc.).