Deshuai Zhen , Shaoqi Zhang , Aofeng Yang , Le Li , Qingyun Cai , Craig A. Grimes , Yu Liu
{"title":"用于体内检测和成像 Fe3+ 的 PEDOT 增强型共价有机框架 (COF) 荧光探针","authors":"Deshuai Zhen , Shaoqi Zhang , Aofeng Yang , Le Li , Qingyun Cai , Craig A. Grimes , Yu Liu","doi":"10.1016/j.ijbiomac.2023.129104","DOIUrl":null,"url":null,"abstract":"<div><p>Simple and accurate <em>in vivo</em> monitoring of Fe<sup>3+</sup><span> is essential for gaining a better understanding of its role in physiological and pathological processes. A novel fluorescent probe was synthesized </span><em>via in situ</em><span> solid-state polymerization of 3,4-ethylenedioxythiophene (PEDOT) in the pore channels of a covalent organic framework (COF). The PEDOT@COF fluorescent probe exhibited an absolute quantum yield (QY) 3 times higher than COF. In the presence of Fe</span><sup>3+</sup><span> the PEDOT@COF 475 nm fluorescence emission, 365 nm excitation, is quenched within 180 s. Fluorescence quenching is linear with Fe</span><sup>3+</sup><span> in the concentration range of 0–960 μM, with a detection limit of 0.82 μM. The fluorescence quenching mechanism was attributed to inner filter effect (IEF), photoinduced electron transfer (PET) and static quenching (SQE) between PEDOT@COF and Fe</span><sup>3+</sup>. A paper strip-based detector was designed to facilitate practical applicability, and the PEDOT@COF probe successfully applied to fluorescence imaging of Fe<sup>3+</sup> levels <em>in vivo</em>. This work details a tool of great promise for enabling detailed investigations into the role of Fe<sup>3+</sup> in physiological and pathological diseases.</p></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":null,"pages":null},"PeriodicalIF":7.7000,"publicationDate":"2023-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"A PEDOT enhanced covalent organic framework (COF) fluorescent probe for in vivo detection and imaging of Fe3+\",\"authors\":\"Deshuai Zhen , Shaoqi Zhang , Aofeng Yang , Le Li , Qingyun Cai , Craig A. Grimes , Yu Liu\",\"doi\":\"10.1016/j.ijbiomac.2023.129104\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Simple and accurate <em>in vivo</em> monitoring of Fe<sup>3+</sup><span> is essential for gaining a better understanding of its role in physiological and pathological processes. A novel fluorescent probe was synthesized </span><em>via in situ</em><span> solid-state polymerization of 3,4-ethylenedioxythiophene (PEDOT) in the pore channels of a covalent organic framework (COF). The PEDOT@COF fluorescent probe exhibited an absolute quantum yield (QY) 3 times higher than COF. In the presence of Fe</span><sup>3+</sup><span> the PEDOT@COF 475 nm fluorescence emission, 365 nm excitation, is quenched within 180 s. Fluorescence quenching is linear with Fe</span><sup>3+</sup><span> in the concentration range of 0–960 μM, with a detection limit of 0.82 μM. The fluorescence quenching mechanism was attributed to inner filter effect (IEF), photoinduced electron transfer (PET) and static quenching (SQE) between PEDOT@COF and Fe</span><sup>3+</sup>. A paper strip-based detector was designed to facilitate practical applicability, and the PEDOT@COF probe successfully applied to fluorescence imaging of Fe<sup>3+</sup> levels <em>in vivo</em>. This work details a tool of great promise for enabling detailed investigations into the role of Fe<sup>3+</sup> in physiological and pathological diseases.</p></div>\",\"PeriodicalId\":333,\"journal\":{\"name\":\"International Journal of Biological Macromolecules\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":7.7000,\"publicationDate\":\"2023-12-30\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International Journal of Biological Macromolecules\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0141813023060038\",\"RegionNum\":1,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of Biological Macromolecules","FirstCategoryId":"92","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0141813023060038","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
A PEDOT enhanced covalent organic framework (COF) fluorescent probe for in vivo detection and imaging of Fe3+
Simple and accurate in vivo monitoring of Fe3+ is essential for gaining a better understanding of its role in physiological and pathological processes. A novel fluorescent probe was synthesized via in situ solid-state polymerization of 3,4-ethylenedioxythiophene (PEDOT) in the pore channels of a covalent organic framework (COF). The PEDOT@COF fluorescent probe exhibited an absolute quantum yield (QY) 3 times higher than COF. In the presence of Fe3+ the PEDOT@COF 475 nm fluorescence emission, 365 nm excitation, is quenched within 180 s. Fluorescence quenching is linear with Fe3+ in the concentration range of 0–960 μM, with a detection limit of 0.82 μM. The fluorescence quenching mechanism was attributed to inner filter effect (IEF), photoinduced electron transfer (PET) and static quenching (SQE) between PEDOT@COF and Fe3+. A paper strip-based detector was designed to facilitate practical applicability, and the PEDOT@COF probe successfully applied to fluorescence imaging of Fe3+ levels in vivo. This work details a tool of great promise for enabling detailed investigations into the role of Fe3+ in physiological and pathological diseases.
期刊介绍:
The International Journal of Biological Macromolecules is a well-established international journal dedicated to research on the chemical and biological aspects of natural macromolecules. Focusing on proteins, macromolecular carbohydrates, glycoproteins, proteoglycans, lignins, biological poly-acids, and nucleic acids, the journal presents the latest findings in molecular structure, properties, biological activities, interactions, modifications, and functional properties. Papers must offer new and novel insights, encompassing related model systems, structural conformational studies, theoretical developments, and analytical techniques. Each paper is required to primarily focus on at least one named biological macromolecule, reflected in the title, abstract, and text.