α-曼戈斯汀通过增加自噬蛋白的表达,减少高糖对人脐静脉内皮细胞的损伤。

IF 2.1 4区 医学 Q3 MEDICINE, RESEARCH & EXPERIMENTAL Iranian Journal of Basic Medical Sciences Pub Date : 2024-01-01 DOI:10.22038/IJBMS.2023.71019.15425
Farhad Eisvand, Kasra Rezvani, Hossein Hosseinzadeh, Bibi Marjan Razavi
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引用次数: 0

摘要

目标:糖尿病是一种因葡萄糖代谢障碍而导致的慢性疾病。在高血糖状态下,氧化应激和抗氧化酶之间的平衡被打破,导致氧化损伤和细胞死亡。此外,自噬功能受损会导致细胞内储存功能失调的蛋白质和细胞器。因此,自噬的细胞保护功能可能会受到高糖条件的破坏。α-山竹素(A-MG)是从山竹果中提纯的一种重要的氧杂蒽酮。研究表明,α-山竹果素具有不同的药理作用,包括抗氧化、抗肥胖和抗糖尿病:我们评估了 A-MG 对高浓度葡萄糖损害人脐静脉内皮细胞(HUVECs)自噬反应的保护作用。用不同浓度的葡萄糖(5-60 mM)和 A-MG(1.25-10 μM)处理 HUVECs 三天。然后,用 60 mM 葡萄糖+2.5 μM A-MG 处理 HUVEC,测量其活力、ROS 和 NO 含量。最后,用 Western 印迹法评估 LC3、SIRT1 和 beclin 1 等自噬蛋白的水平:结果表明:高葡萄糖条件(60 mM)降低了 HUVECs 的活力,增加了 ROS 和 NO 的含量。此外,当 HUVEC 暴露于高浓度葡萄糖时,LC3、SIRT1 和 beclin 1 蛋白水平下降。A-MG 逆转了这些不利影响,并提高了自噬蛋白水平:我们的数据表明,A-MG 可通过减少 ROS 和 NO 的生成以及增加自噬蛋白的表达来保护 HUVECs 免受高浓度葡萄糖的影响。
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Alpha-mangostin decreases high glucose-induced damage on human umbilical vein endothelial cells by increasing autophagic protein expression.

Objectives: Diabetes is a chronic disorder that occurs as a result of impaired glucose metabolism. In hyperglycaemic states, the balance between oxidative stress and antioxidant enzymes is disrupted leading to oxidative damage and cell death. In addition, impaired autophagy leads to the storage of dysfunctional proteins and cellular organelles in the cell. Hence, the cytoprotective function of autophagy may be disrupted by high glucose conditions. Alpha-mangostin (A-MG) is an essential xanthone purified from the mangosteen fruit. The different pharmacological benefits of alpha-mangostin, including antioxidant, anti-obesity, and antidiabetic, were demonstrated.

Materials and methods: We evaluated the protective influence of A-MG on autophagic response impaired by high concentrations of glucose in human umbilical vein endothelial cells (HUVECs). The HUVECs were treated with various glucose concentrations (5-60 mM) and A-MG (1.25-10 μM) for three days. Then, HUVECs were treated with 60 mM of glucose+2.5 μM of A-MG to measure viability, ROS, and NO content. Finally, the levels of autophagic proteins including LC3, SIRT1, and beclin 1 were evaluated by western blot.

Results: The results expressed that high glucose condition (60 mM) decreased viability and increased ROS and NO content in HUVECs. In addition, LC3, SIRT1, and beclin 1 protein levels declined when HUVECs were exposed to the high concentration of glucose. A-MG reversed these detrimental effects and elevated autophagic protein levels.

Conclusion: Our data represent that A-MG protects HUVECs against high glucose conditions by decreasing ROS and NO generation as well as increasing the expression of autophagy proteins.

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来源期刊
Iranian Journal of Basic Medical Sciences
Iranian Journal of Basic Medical Sciences MEDICINE, RESEARCH & EXPERIMENTAL-PHARMACOLOGY & PHARMACY
CiteScore
4.00
自引率
4.50%
发文量
142
审稿时长
6-12 weeks
期刊介绍: The Iranian Journal of Basic Medical Sciences (IJBMS) is a peer-reviewed, monthly publication by Mashhad University of Medical Sciences (MUMS), Mashhad, Iran . The Journal of "IJBMS” is a modern forum for scientific communication. Data and information, useful to investigators in any discipline in basic medical sciences mainly including Anatomical Sciences, Biochemistry, Genetics, Immunology, Microbiology, Pathology, Pharmacology, Pharmaceutical Sciences, and Physiology, will be published after they have been peer reviewed. This will also include reviews and multidisciplinary research.
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