去泛醇芯/壳电纺纳米纤维的伤口愈合效果:氧化应激对伤口愈合的影响

IF 2.1 4区 医学 Q3 MEDICINE, RESEARCH & EXPERIMENTAL Iranian Journal of Basic Medical Sciences Pub Date : 2024-01-01 DOI:10.22038/IJBMS.2023.71412.15526
Seyede Sahar Hashemi, Mahmoud Najari, Milad Parvin, Mohammad Mehdi Kalani, Majid Assadi, Ramin Seyedian, Sasan Zaeri
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引用次数: 0

摘要

研究目的我们了解氧化应激在伤口愈合中的不利作用以及右泛酰苯酚(Dex)的抗氧化特性,因此我们旨在生产载 Dex 的电纺芯/壳纳米纤维,用于伤口愈合研究。新颖之处在于测量伤口中的氧化应激,以了解 Dex 负载纤维如何影响氧化应激:材料:含 6% Dex(w/v)的 TPVA 溶液(芯)和 PVA/壳聚糖溶液(壳)同轴电纺,壳溶液的注入率可变。然后测试了纤维的理化特性、药物释放情况以及对伤口愈合的影响。测量了组织脂质过氧化水平和超氧化物歧化酶活性:以 0.3 毫升/小时的壳注射速率生产的纤维(F3 纤维)显示出平均直径为 252 纳米的核/壳结构、高亲水性(平衡时膨胀 157%)和低失重(13.6%)。根据 Korsmeyer-Peppas 模型(R2 = 0.94,n = 0.37),F3 纤维的 Dex 释放似乎受 Fickian 机制支配。在伤口愈合后第 14 天,与纯 F3 纤维相比,添加了 Dex 的 F3 纤维在宏观和微观层面上显著提高了成纤维细胞的活力(128.4%),并加快了伤口愈合。一个重要的发现是,在添加了 Dex 的 F3 纤维处理过的伤口组织中,丙二醛(0.39 毫摩尔/毫克蛋白质)水平明显降低,超氧化物歧化酶(5.29 单位/毫克蛋白质)活性明显提高:结论:负载 Dex 的芯/壳纤维提供了具有持续释放特性的纳米级支架,可显著降低组织氧化应激。这一发现表明了降低氧化应激对伤口正常愈合的重要性。
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Wound healing effects of dexpanthenol-loaded core/shell electrospun nanofibers: Implication of oxidative stress in wound healing.

Objectives: Knowing the detrimental role of oxidative stress in wound healing and the anti-oxidant properties of Dexpanthenol (Dex), we aimed to produce Dex-loaded electrospun core/shell nanofibers for wound healing study. The novelty was measuring oxidative stress in wounds to know how oxidative stress was affected by Dex-loaded fibers.

Materials and methods: TPVA solution containing Dex 6% (w/v) (core) and PVA/chitosan solution (shell) were coaxially electrospun with variable injection rates of the shell solution. Fibers were then tested for physicochemical properties, drug release profile, and effects on wound healing. Levels of tissue lipid peroxidation and superoxide dismutase activity were measured.

Results: Fibers produced at shell injection rate of 0.3 ml/hr (F3 fibers) showed core/shell structure with an average diameter of 252 nm, high hydrophilicity (swelling: 157% at equilibrium), and low weight loss (13.6%). Dex release from F3 fibers seemed to be ruled by the Fickian mechanism based on the Korsmeyer-Peppas model (R2 = 0.94, n = 0.37). Dex-loaded F3 fibers promoted fibroblast viability (128.4%) significantly on day 5 and also accelerated wound healing compared to the neat F3 fibers at macroscopic and microscopic levels on day 14 post-wounding. The important finding was a significant decrease in malondialdehyde (0.39 nmol/ mg protein) level and an increase in superoxide dismutase (5.29 unit/mg protein) activity in Dex-loaded F3 fiber-treated wound tissues.

Conclusion: Dex-loaded core/shell fibers provided nano-scale scaffolds with sustained release profile that significantly lowered tissue oxidative stress. This finding pointed to the importance of lowering oxidative stress to achieve proper wound healing.

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来源期刊
Iranian Journal of Basic Medical Sciences
Iranian Journal of Basic Medical Sciences MEDICINE, RESEARCH & EXPERIMENTAL-PHARMACOLOGY & PHARMACY
CiteScore
4.00
自引率
4.50%
发文量
142
审稿时长
6-12 weeks
期刊介绍: The Iranian Journal of Basic Medical Sciences (IJBMS) is a peer-reviewed, monthly publication by Mashhad University of Medical Sciences (MUMS), Mashhad, Iran . The Journal of "IJBMS” is a modern forum for scientific communication. Data and information, useful to investigators in any discipline in basic medical sciences mainly including Anatomical Sciences, Biochemistry, Genetics, Immunology, Microbiology, Pathology, Pharmacology, Pharmaceutical Sciences, and Physiology, will be published after they have been peer reviewed. This will also include reviews and multidisciplinary research.
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