伊朗患者乳腺肿瘤组织中 Beclin1 和 mTOR 基因及 p62 蛋白表达的评估。

IF 1.5 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Molecular Biology Research Communications Pub Date : 2024-01-01 DOI:10.22099/mbrc.2023.47597.1837
Maryam Adelipour, Mahshid Naghashpour, Mohammad Reza Roshanazadeh, Hadi Chenaneh, Asma Mohammadi, Pegah Pourangi, Seyed Rouhollah Miri, Atefeh Zahedi, Mahmood Haghighatnezhad, Sahar Golabi
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引用次数: 0

摘要

自噬是一种细胞过程,对肿瘤细胞的命运起着重要作用。了解自噬在癌症治疗中的作用是一项重大挑战,尤其是对于作为女性唯一致死原因的乳腺癌而言。在这项研究中,我们评估了乳腺肿瘤中 mTOR 和 Beclin1 的基因表达以及 p62 蛋白的水平,并与对照组进行了比较。为了探索自噬在乳腺癌中的作用,我们从 41 例新的乳腺癌患者身上获取了肿瘤活检样本。我们从每个活检组织中提取了总 RNA,并使用实时 PCR 对 Beclin1 和 mTOR 特异性 RNA 的表达进行了量化。此外,我们还使用免疫组化技术评估了石蜡包埋肿瘤组织中 p62 蛋白的表达。数据显示,与对照组相比,肿瘤组织中的 Beclin1 上调,而 mTOR 下调。p62的表达与Beclin1/mTOR的表达分别呈负相关和正相关,证实了肿瘤组织中自噬的激活。然而,自噬标记物与肿瘤大小、分级和分期没有相关性。研究结果表明,乳腺肿瘤组织中存在自噬激活现象,这表明自噬可作为乳腺癌治疗的靶点。
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Evaluation of Beclin1 and mTOR genes and p62 protein expression in breast tumor tissues of Iranian patients.

Autophagy is a cellular process that plays a major role in the fate of tumor cells. Understanding the role of autophagy in cancer therapy is a major challenge, particularly for breast cancer as the sole top cause of mortality among women. In this study, we evaluated the gene expression of mTOR and Beclin1 and the levels of p62 protein, in breast tumors and compared them to a control condition. To explore the role of autophagy in breast cancer, we acquired tumor biopsies from 41 new cases of breast cancer patients. We extracted total RNA from each biopsy and used real-time PCR to quantify Beclin1 and mTOR-specific RNA expression. In addition, we evaluated the expression of the p62 protein in paraffin-embedded tumor tissue using the immunohistochemistry technique. The data revealed an upregulation of Beclin1 and a downregulation of mTOR in tumor tissues compared to the control condition. The correlation between p62 expression and Beclin1/mTOR showed a negative and positive correlation, respectively, confirming autophagy activation in the tumor tissues. However, there was no correlation between autophagy markers and tumor size, grade and stage. The findings revealed that autophagy activation was found in breast tumor tissues, suggesting that autophagy can be a target for breast cancer therapy.

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来源期刊
Molecular Biology Research Communications
Molecular Biology Research Communications BIOCHEMISTRY & MOLECULAR BIOLOGY-
CiteScore
3.00
自引率
0.00%
发文量
12
期刊介绍: “Molecular Biology Research Communications” (MBRC) is an international journal of Molecular Biology. It is published quarterly by Shiraz University (Iran). The MBRC is a fully peer-reviewed journal. The journal welcomes submission of Original articles, Short communications, Invited review articles, and Letters to the Editor which meets the general criteria of significance and scientific excellence in all fields of “Molecular Biology”.
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