胰岛素疗法对糖尿病大鼠胫骨宏观、微结构和力学性能的影响

IF 2.4 Q3 ENDOCRINOLOGY & METABOLISM Current diabetes reviews Pub Date : 2024-01-01 DOI:10.2174/0115733998270859231117091741
Pedro Henrique Justino Oliveira Limirio, Nilson Ferreira De Oliveira Neto, Jessyca Figueira Venâncio, Camila Rodrigues Borges Linhares, Priscilla Barbosa Ferreira Soares, Paula Dechichi
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摘要

背景:本研究评估了 1 型糖尿病(T1DM)大鼠胫骨的宏观结构、力学性能和骨微结构:本研究评估了 1 型糖尿病(T1DM)大鼠胫骨的宏观结构、力学性能和骨微结构:18只大鼠分为三组(n=6):非糖尿病组(ND)、糖尿病组(D)和糖尿病+胰岛素组(DI)。通过链脲佐菌素诱发 T1DM;每天注射胰岛素(4IU)。动物在诱导后 35 天安乐死。取出胫骨,使用宏观、微型计算机断层扫描(micro-CT)和三点弯曲技术进行分析。宏观分析测量了近端-远端长度(PD)、近端(AP-P)和远端(AP-D)骨骺的前后厚度(AP)以及近端(LM-P)和远端(LM-D)骨骺的外侧-内侧厚度(LM)。显微 CT 分析封闭了孔隙率、组织矿物质密度和皮质厚度。三点弯曲试验测量了最大强度、能量和刚度:宏观分析表明,与 ND 和 DI 相比,D 的长度和厚度(AP 和 AP-P)较小。在显微 CT 中,DI 的皮质厚度大于 D。机械分析表明,D 的强度低于其他组:结论:T1DM会降低骨生长和机械强度。糖尿病大鼠的胰岛素治疗可改善骨生长和抗骨折能力,使糖尿病骨与正常血糖动物相似。
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Insulin Therapy on Bone Macroscopic, Microarchitecture, and MechanicalProperties of Tibia in Diabetic Rats.

Background: This study evaluated tibia's macroscopic structure, mechanical properties, and bone microarchitecture in rats with type 1 diabetes mellitus (T1DM).

Methods: Eighteen animals were divided into three groups (n=6): Non-diabetic (ND), diabetic (D), and diabetic+insulin (DI). T1DM was induced by streptozotocin; insulin was administered daily (4IU). The animals were euthanized 35 days after induction. The tibiae were removed and analyzed using macroscopic, micro-computed tomography (micro-CT) and three-point bending. The macroscopic analysis measured proximal-distal length (PD), antero-posterior thickness (AP) of proximal (AP-P) and distal (AP-D) epiphysis, and lateral-medial thickness (LM) of proximal (LM-P) and distal (LM-D) epiphysis. Micro-CT analysis closed porosity, tissue mineral density, and cortical thickness. The three-point bending test measured maximum strength, energy, and stiffness.

Results: The macroscopic analysis showed that D presented smaller measures of length and thickness (AP and AP-P) than ND and DI. More extensive measurements were observed of LM and AP-D thickness in DI than in D. In micro-CT, DI showed larger cortical thickness than D. Mechanical analysis showed lower strength in D than in other groups.

Conclusions: T1DM reduces bone growth and mechanical strength. Insulin therapy in diabetic rats improved bone growth and fracture resistance, making diabetic bone similar to normoglycemic animals.

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来源期刊
Current diabetes reviews
Current diabetes reviews ENDOCRINOLOGY & METABOLISM-
CiteScore
6.30
自引率
0.00%
发文量
158
期刊介绍: Current Diabetes Reviews publishes frontier reviews on all the latest advances on diabetes and its related areas e.g. pharmacology, pathogenesis, complications, epidemiology, clinical care, and therapy. The journal"s aim is to publish the highest quality review articles dedicated to clinical research in the field. The journal is essential reading for all researchers and clinicians who are involved in the field of diabetes.
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