尿囊素在脑缺血再灌注损伤中的分子机制

Deyuan Qin, Jiangcun Wei, Xiumei Ma, Bing Qing, Meiyan Qiu, Peng Yang, Zhengteng Yang
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摘要

壮医药中的一味名贵药材--泌尿草,具有清热、解毒、利尿、消肿的功效。本研究探讨了荨麻对脑缺血再灌注损伤(CIRI)的治疗作用和分子机制。将 60 只成年 SD 大鼠随机分为四组:假手术组、右侧大脑中动脉闭塞(MCAO)模型组、5 克/千克 P. urinaria 低剂量组和 10 克/千克 P. urinaria 高剂量组,每组 15 只。连续给药七天后,建立右侧 MCAO 大鼠模型,模拟 CIRI。用 Longa 评分法评估神经功能,用 TTC 染色法评估脑梗塞面积,并通过 ELISA 检测细胞因子水平(IL-1β、TNF-α、IL-6)。通过 Western 印迹分析了脑组织中 PI3K 和 AKT 蛋白的表达。结果显示,与 MCAO 模型组相比,低剂量和高剂量尿囊素组的神经功能评分均有明显改善。在脑缺血 72 小时后,P. urinaria 能明显缩小脑梗死面积。与 MCAO 模型组相比,两个治疗组的炎症细胞因子(IL-1β、TNF-α、IL-6)水平均有所下降。两个治疗组中 PI3K 和 AKT 蛋白的表达均有明显增加。总之,P. urinaria 通过抑制炎症反应、改善神经功能和减轻脑损伤对 CIRI 具有保护作用。
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Molecular Mechanisms of Phyllanthus urinaria in Cerebral Ischemia-Reperfusion Injury
Phyllanthus urinaria (P. urinaria), a prominent herb in traditional Zhuang medicine, is characterized by its heat clearance, detoxification, diuresis, and detumescence. This study investigates the therapeutic effects and molecular mechanisms of P. urinaria on cerebral ischemia-reperfusion injury (CIRI). 60 adult SD rats were randomly divided into four groups: a sham operation group, a model group of right middle cerebral artery occlusion (MCAO), a low-dose group treated with P. urinaria at 5 g/kg, and a high-dose group treated with P. urinaria at 10 g/kg, each consisting of 15 rats. After seven days of continuous administration, a rat model of the right MCAO was established to simulate CIRI. Nerve function was assessed using the Longa scoring method, the cerebral infarction area was evaluated with TTC staining, and cytokine levels (IL-1β, TNF-α, IL-6) were measured via ELISA. PI3K and AKT protein expression in brain tissues was analyzed by Western blot. The results revealed a significant improvement in neurological function scores in both low and high-dose P. urinaria groups compared to the MCAO model group. Treatment with P. urinaria led to a notable reduction in cerebral infarction area after 72 hours of cerebral ischemia. The levels of inflammatory cytokines (IL-1β, TNF-α, IL-6) were decreased in both treatment groups compared to the MCAO model group. There was a significant increase in the expression of PI3K and AKT proteins in both treatment groups. In conclusion, P. urinaria exhibits a protective effect against CIRI by inhibiting inflammatory responses, improving neurological function, and reducing brain injury.
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来源期刊
Journal of Biobased Materials and Bioenergy
Journal of Biobased Materials and Bioenergy 工程技术-材料科学:生物材料
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60
审稿时长
6 months
期刊最新文献
Molecular Mechanisms of Phyllanthus urinaria in Cerebral Ischemia-Reperfusion Injury Preparation and Surface Modification of Nanocellulose and Its Adsorption Performance on Cu2+ Overview of Marginal Lands in Henan Province, China and Evaluation of Development Potential of Biomass-To-Liquid Fuels Bee Pollen: An Antioxidant and Antimicrobial Agent for Beef Sausage Physiological and Photosynthetic Characteristics of Pumpkin Seedlings Cultivated Under Low-Light Stress
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