Anna Och, Agata Ćwikła, Karolina Orzechowska, A. Cwener
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引用次数: 0
摘要
引言和目的。评估植物提取物的抗氧化活性是寻找新的化学预防物质的一个重要研究方向。本研究旨在证明小檗(Berberis jaeschkeana C. K. Schneid)果实甲醇提取物的抗氧化潜力。材料和方法。进行了分光光度测试:测试提取物中多酚的总含量(TPC),测试提取物中黄酮类化合物的总含量(TFC)。使用改进的 ABTS+- 脱色试验测定抗自由基活性。结果TPC 提取物的含量为 43.52±2.37 mg/g,TFC 提取物的含量为 6.08±0.48 mg/g。ABTS 活性测试显示为 31.15 mg/g,以每克干提取物含 mg Trolox 表示。结论经评估,红叶小檗果实的甲醇提取物具有抗氧化特性,使其成为潜在的文明病化学预防剂。
Evaluation of the antioxidant activity of Berberis jaeschkeana C. K. Schneid. fruits using the ABTS assay
Introduction and aim. The assessment of the antioxidant activity of plant extracts is an important research direction in the search for new chemopreventive substances. The aim of this study was to demonstrate the antioxidant potential of the methanol extract of Berberis jaeschkeana C. K. Schneid fruit. Material and methods. Spectrophotometric tests were carried out: testing of the total content of polyphenols (TPC) in extracts, testing of the total content of flavonoids (TFC) in extracts. Antiradical activity was determined using the improved ABTS+• decolorization test with modifications. Results. TPC extracts was determined at the level of 43.52±2.37 mg/g, while TFC extracts was determined at the level 6.08±0.48 mg/g. The ABTS activity test showed 31.15 mg/g expressed as mg Trolox per g of dry extract. Conclusion. It was assessed that the methanolic extract of the fruit of Berberis jaeschkeana C. K. Schneid. has an antioxidant properties, what makes it a potential chemopreventive agent in civilizational diseases.