常春藤甲醇叶提取物的营养成分和气相色谱-质谱(GC-MS)分析

Rosephine Enadeghe, A. E. Omoregie
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引用次数: 0

摘要

在西非的民族医药中,常春藤(TI)被用于治疗溃疡、疟疾、炎症和伤口。尽管它被广泛使用,但其营养状况在很大程度上仍未确定。本研究旨在评估 TI 的营养和植物化学成分。采用 AOAC 方法对 TI 的甲醇叶提取物进行了近似分析。还使用原子吸收光谱仪(AAS)对植物的甲醇叶提取物进行了元素分析。生物活性成分的鉴定和定量采用气相色谱-质谱(GC-MS)单相离子模式。检测到的化合物光谱与美国国家标准与技术研究院(NIST)的已知化合物进行了比对。近似分析表明,它含有丰富的主要营养成分,如碳水化合物(37.70±0.05%)、蛋白质(10.94±0.03%)、脂肪(7.97±0.12%)、纤维(0.95±0.01%)、灰分(1.42±0.01%)和水分含量(41.02±0.21%)。提取物的矿物质分析表明,其中含有人体必需的矿物质,如钙(641.2±1.20 毫克/100 克)、铜(6.28±0.02 毫克/100 克)、镁(186.16±0.29 毫克/100 克)、铁(17.13±0.06 毫克/100 克)、锰(3.47±0.01 毫克/100 克)和锌(3.82±0.01 毫克/100 克),这些矿物质是新陈代谢过程和怀孕所必需的。通过气相色谱-质谱(GC-MS)检测出 47 种植物化学物质,其中最主要的是 1, 2, 3- 苯三酚(26.31%)、邻苯二甲酸二乙酯(14.93%)、D-阿洛糖(6.96%)、脱水-α-d-半乳糖呋喃糖(5.09%)和甘油(6.28%)。研究表明,象牙果是营养物质、矿物质和多种有益生物活性化合物的有效来源,可作为药物开发所需的潜在药物靶点。
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NUTRITIONAL COMPOSITION AND GAS CHROMATOGRAPHY-MASS SPECTROMETRY (GC-MS) ANALYSIS OF METHANOL LEAF EXTRACT OF Terminalia ivorensis
Terminalia ivorensis (TI) is used in West African ethnomedicine for the treatment of ulcers, malaria, inflammation, and wounds. Despite its widespread use, its nutritional status remains largely undetermined. This study was undertaken with the aim to evalute the nutritional and phytochemical composition of TI. Proximate analysis of the methanol leaf extracts of TI was carried out using the AOAC methods. Elemental analysis of the methanol leaf extracts of the plant was also carried out using an Atomic Absorption Spectrometer (AAS). Identification and quantification of the bioactive components were determined by Gas Chromatography-Mass Spectrometry (GC-MS) single-phase ion mode. Spectra from detected compounds were matched with known compounds of the National Institute of Standards and Technology (NIST). Proximate analysis reveals that it is rich in primary nutrients such as carbohydrates (37.70±0.05%), proteins (10.94±0.03%), fats (7.97±0.12%), fibre (0.95±0.01%), ash (1.42±0.01%) and moisture content (41.02±0.21%). Mineral analysis of the extract revealed the presence of essential minerals such as calcium (641.2±1.20 mg/100g), copper (6.28±0.02 mg/100g), magnesium (186.16±0.29 mg/100g), iron (17.13±0.06 mg/100g), manganese (3.47±0.01 mg/100g), and zinc (3.82±0.01 mg/100g), which are essential for the metabolic processes and pregnancy. Forty-Seven (47) phytochemical compounds were detected by GC-MS including 1, 2, 3- Benzenetriol (26.31%), Diethyl Phthalate (14.93%), D-Allose (6.96%), Anhydro-.alpha-d-galactofuranose (5.09), Glycerin (6.28%) as the most predominant. The study revealed that Terminalia ivorensis could be a useful source of nutrients, minerals, and several helpful bioactive compounds that may serves as potential drug target needed for drug development
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