Gulnaz Shaymullina, R. Safin, Olga Egorova, A. Valiev, Marat Kalimullin
{"title":"研究芽孢杆菌属内生细菌对春小麦和大豆种子生长和抗逆性的影响","authors":"Gulnaz Shaymullina, R. Safin, Olga Egorova, A. Valiev, Marat Kalimullin","doi":"10.12737/2073-0462-2023-53-59","DOIUrl":null,"url":null,"abstract":"The studies were carried out to assess the indicators of seed germination, root length, level of lipid peroxidation, content of photosynthetic pigments and free proline in monocotyledonous and dicotyledonous crops when seeds were treated with strains of endophytic bacteria of the genus Bacillus. The scheme of vegetation experiments included modeling of 30% drought on sand and pre-sowing treatment of seeds of spring wheat variety Yoldyz and soybean variety Sultana with endophytic bacteria: Bacillus subtilis KS-55 AU, Bacillus cereus KS-56 AU, Bacillus pumilus KS-57 AU, Bacillus megaterium KS- 58 AU, Bacillus cereus KS-59 AU, Bacillus megaterium KS-60 AU and Bacillus pumilus KS-61 AU. Bacteria better stimulated root growth when using a titer of 1×106 cells in 1 ml of the drug (the increase was 0.6 cm on soybean with Bacillus subtilis KS-55 AU and Bacillus megaterium KS-60 AU and 0.4 cm on wheat with Bacillus cereus KS -56 AU, Bacillus cereus KS-59 AU and Bacillus pumilus KS-61 AU). In wheat, the Bacillus subtilis KS-55 AU strain stood out, treatment with which contributed to an increase in germination by 30%, a decrease in the level of lipid peroxidation by 46%, an increase in the amount of free proline by 148% and photosynthetic pigments by 6%, relative to the control variant. On soybean plants, the strain Bacillus subtilis KS-55 AU contributed to an increase in germination by 40%, Bacillus cereus KS-59 AU contributed to a decrease in the level of lipid peroxidation by 47% and the accumulation of proline by 73%, and Bacillus pumilus KS-61 AU increased the amount of total chlorophyll by 141%, relative to the control variant. Thus, we can conclude that the above strains of endophytic bacteria improve the growth and development of spring wheat and soybean seedlings, and also serve as inducers of crop resistance to soil drought.","PeriodicalId":23526,"journal":{"name":"Vestnik of Kazan State Agrarian University","volume":"39 2","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2023-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"STUDY OF THE INFLUENCE OF ENDOPHYTIC BACTERIA OF THE GENUS BACILLUS ON THE GROWTH AND STRESS RESISTANCE OF SPRING WHEAT AND SOYBEAN SEEDLS\",\"authors\":\"Gulnaz Shaymullina, R. Safin, Olga Egorova, A. Valiev, Marat Kalimullin\",\"doi\":\"10.12737/2073-0462-2023-53-59\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The studies were carried out to assess the indicators of seed germination, root length, level of lipid peroxidation, content of photosynthetic pigments and free proline in monocotyledonous and dicotyledonous crops when seeds were treated with strains of endophytic bacteria of the genus Bacillus. The scheme of vegetation experiments included modeling of 30% drought on sand and pre-sowing treatment of seeds of spring wheat variety Yoldyz and soybean variety Sultana with endophytic bacteria: Bacillus subtilis KS-55 AU, Bacillus cereus KS-56 AU, Bacillus pumilus KS-57 AU, Bacillus megaterium KS- 58 AU, Bacillus cereus KS-59 AU, Bacillus megaterium KS-60 AU and Bacillus pumilus KS-61 AU. Bacteria better stimulated root growth when using a titer of 1×106 cells in 1 ml of the drug (the increase was 0.6 cm on soybean with Bacillus subtilis KS-55 AU and Bacillus megaterium KS-60 AU and 0.4 cm on wheat with Bacillus cereus KS -56 AU, Bacillus cereus KS-59 AU and Bacillus pumilus KS-61 AU). In wheat, the Bacillus subtilis KS-55 AU strain stood out, treatment with which contributed to an increase in germination by 30%, a decrease in the level of lipid peroxidation by 46%, an increase in the amount of free proline by 148% and photosynthetic pigments by 6%, relative to the control variant. On soybean plants, the strain Bacillus subtilis KS-55 AU contributed to an increase in germination by 40%, Bacillus cereus KS-59 AU contributed to a decrease in the level of lipid peroxidation by 47% and the accumulation of proline by 73%, and Bacillus pumilus KS-61 AU increased the amount of total chlorophyll by 141%, relative to the control variant. Thus, we can conclude that the above strains of endophytic bacteria improve the growth and development of spring wheat and soybean seedlings, and also serve as inducers of crop resistance to soil drought.\",\"PeriodicalId\":23526,\"journal\":{\"name\":\"Vestnik of Kazan State Agrarian University\",\"volume\":\"39 2\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2023-12-28\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Vestnik of Kazan State Agrarian University\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.12737/2073-0462-2023-53-59\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Vestnik of Kazan State Agrarian University","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.12737/2073-0462-2023-53-59","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
摘要
研究的目的是评估单子叶和双子叶作物种子经芽孢杆菌属内生细菌菌株处理后的种子发芽率、根长、脂质过氧化程度、光合色素含量和游离脯氨酸等指标。植被实验方案包括在沙地上模拟 30% 的干旱,并在播种前用内生细菌处理春小麦品种 Yoldyz 和大豆品种 Sultana 的种子:枯草芽孢杆菌 KS-55 AU、蜡样芽孢杆菌 KS-56 AU、枯草芽孢杆菌 KS-57 AU、巨大芽孢杆菌 KS- 58 AU、蜡样芽孢杆菌 KS-59 AU、巨大芽孢杆菌 KS-60 AU 和枯草芽孢杆菌 KS-61 AU。在 1 毫升药物中使用滴度为 1×106 个细胞的细菌能更好地刺激根系生长(枯草芽孢杆菌 KS-55 AU 和巨大芽孢杆菌 KS-60 AU 能使大豆根系生长 0.6 厘米,枯草芽孢杆菌 KS -56 AU、枯草芽孢杆菌 KS-59 AU 和枯草芽孢杆菌 KS-61 AU 能使小麦根系生长 0.4 厘米)。在小麦中,枯草芽孢杆菌 KS-55 AU 菌株表现突出,与对照变体相比,该菌株可使小麦发芽率提高 30%,脂质过氧化水平降低 46%,游离脯氨酸量增加 148%,光合色素增加 6%。在大豆植株上,枯草芽孢杆菌 KS-55 AU 菌株使发芽率提高了 40%,蜡样芽孢杆菌 KS-59 AU 菌株使脂质过氧化水平降低了 47%,脯氨酸积累了 73%,而枯草芽孢杆菌 KS-61 AU 菌株则使叶绿素总量比对照变体提高了 141%。因此,我们可以得出结论,上述内生细菌菌株能改善春小麦和大豆幼苗的生长发育,同时也是作物抵抗土壤干旱的诱导剂。
STUDY OF THE INFLUENCE OF ENDOPHYTIC BACTERIA OF THE GENUS BACILLUS ON THE GROWTH AND STRESS RESISTANCE OF SPRING WHEAT AND SOYBEAN SEEDLS
The studies were carried out to assess the indicators of seed germination, root length, level of lipid peroxidation, content of photosynthetic pigments and free proline in monocotyledonous and dicotyledonous crops when seeds were treated with strains of endophytic bacteria of the genus Bacillus. The scheme of vegetation experiments included modeling of 30% drought on sand and pre-sowing treatment of seeds of spring wheat variety Yoldyz and soybean variety Sultana with endophytic bacteria: Bacillus subtilis KS-55 AU, Bacillus cereus KS-56 AU, Bacillus pumilus KS-57 AU, Bacillus megaterium KS- 58 AU, Bacillus cereus KS-59 AU, Bacillus megaterium KS-60 AU and Bacillus pumilus KS-61 AU. Bacteria better stimulated root growth when using a titer of 1×106 cells in 1 ml of the drug (the increase was 0.6 cm on soybean with Bacillus subtilis KS-55 AU and Bacillus megaterium KS-60 AU and 0.4 cm on wheat with Bacillus cereus KS -56 AU, Bacillus cereus KS-59 AU and Bacillus pumilus KS-61 AU). In wheat, the Bacillus subtilis KS-55 AU strain stood out, treatment with which contributed to an increase in germination by 30%, a decrease in the level of lipid peroxidation by 46%, an increase in the amount of free proline by 148% and photosynthetic pigments by 6%, relative to the control variant. On soybean plants, the strain Bacillus subtilis KS-55 AU contributed to an increase in germination by 40%, Bacillus cereus KS-59 AU contributed to a decrease in the level of lipid peroxidation by 47% and the accumulation of proline by 73%, and Bacillus pumilus KS-61 AU increased the amount of total chlorophyll by 141%, relative to the control variant. Thus, we can conclude that the above strains of endophytic bacteria improve the growth and development of spring wheat and soybean seedlings, and also serve as inducers of crop resistance to soil drought.