Effects of demecolcine and oocyte maturation time on the efficiency in the production of Vietnamese pot-bellied pig embryos

The results of this study showed that the concentration of Demecolcine affected efficiency in the production of Vietnamese pot-bellied pig embryos. The 4 μM Demecolcine group resulted in a greater oocyte extrusion cones rate in comparison to 0, 6, and 8 μM groups (72.03 vs 1.98%, 59.42, and 51.01%, p<0.05, respectively). The cleavage rate and blastocyst rate in the 4 μM Demecolcine group were significantly higher than in the 6 and 8 μM groups (p<0.05). Demecolcine treatment time affected efficiency in the production of Vietnamese pot-bellied cloned pig embryos. The cleavage and blastocyst rates of the 1 h group were significantly higher than that of the 0.5, 2 and 3 h (84.68 vs 70.92%, 73.86, and 60.36%; 24.91 vs 13.62%, 17.26 vs 10.63%, p<0.05, respectively). The oocyte maturation time of porcine oocytes affected the production efficiency of Vietnamese pot-bellied cloned pig embryos. The oocyte extrusion cone rate of the 20 h group was significantly higher than that of the 18 and 22 h groups (85.34 vs 60.04, and 72.03%, p<0.05, respectively). The cleavage rate and blastocyst rate in the 18 h group were significantly lower than that in the 20 and 22 h groups (66.12 vs 85.42, and 84.68%; 13.71 vs 25.48, and 24.91%, p<0.05, respectively). In conclusion, Demecolcine and oocyte maturation time had effects on the efficiency in the production of Vietnamese pot-bellied pig embryos.