超声辅助萃取法分析伊斯金(Rheum ribes L.)植物不同器官的精油和挥发性成分

Hasan Asi̇l, M. Köroğlu
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摘要

Rheum ribes L.属于蓼科,在当地被称为 "ışkın、ucgun、高原香蕉"。本研究采用甲醇:乙酸乙酯混合溶剂进行超声波辅助萃取,从根、茎和叶器官中分离出该植物的挥发性物质。通过水蒸馏法从植物根部提取精油,以确定精油的含量和成分。采用气相色谱-质谱法(GC-MS)分析精油和提取物的含量。植物根提取物中含有香芹酚(40.41%)和γ-萜品烯(22.90%),茎提取物中含有 2,4-二叔丁基苯酚(20.76%)和香芹酚(13.52%),叶提取物中含有 2,4-二叔丁基苯酚(25.87%)和甲酸甲酯(6.87%)。精油中的挥发性成分为 6-丁基-3-甲氧基-2-环己烯-1-酮(30.06%)和 3-蒈烯(16.42%)。在研究该植物提取物和精油中挥发性成分的结构时,发现了可用于生产药用、生物活性、香料、染料材料和香水的多种挥发性成分,这表明该植物可作为一种重要的药用和芳香植物来源。
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Analysis of Essential Oil and Volatile Components of Different Organs of Isgin (Rheum ribes L.) Plant by Ultrasound-Assisted Extraction Method
Rheum ribes L. belongs to the family Polygonaceae and is locally known as "ışkın, ucgun, highland banana". In this study, ultrasound-assisted extraction with a solvent mixture of methanol: ethyl acetate was used to isolate the volatiles of the plant from the root, stem, and leaf organs. The essential oils were extracted from the plant roots by water distillation to determine the essential oil content and composition. GC-MS (Gas Chromatography-Mass Spectrometry) was used to analyse the content of essential oils and extracts. Carvacrol (40.41%) and gamma-terpinene (%22.90) were used in the root extracts of the plant., 2,4-ditert-butylphenol (20.76%) and carvacrol (13.52%) in the stem extracts and 2,4-ditert-butylphenol (25.87%) and methyl formate (6.87%) in the leaf extracts. The volatile components of 6-butyl-3-methoxy-2-cyclohexen-1-one (30.06%) and 3-carene (16.42%) were found in the essential oil. When the structure of the volatile components in both the extract and the essential oil of the plant is examined, the presence of versatile volatile components that can be used in the production of pharmacological, bioactive, flavouring, dye material, and perfume indicated that the plant can be used as an important medicinal and aromatic plant source.
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