粉红色素兼性养甲菌(PPFM)甲醇和乙醇提取物的抗氧化活性、酚类化合物及毒性测定

IF 0.6 Q3 AGRICULTURE, MULTIDISCIPLINARY Pertanika Journal of Tropical Agricultural Science Pub Date : 2023-11-27 DOI:10.47836/pjtas.46.4.21
Nur Isti’anah Ramli, F. Abas, I. S. Ismail, Y. Rukayadi, S. Md Nor
{"title":"粉红色素兼性养甲菌(PPFM)甲醇和乙醇提取物的抗氧化活性、酚类化合物及毒性测定","authors":"Nur Isti’anah Ramli, F. Abas, I. S. Ismail, Y. Rukayadi, S. Md Nor","doi":"10.47836/pjtas.46.4.21","DOIUrl":null,"url":null,"abstract":"Pink-pigmented facultative methylotrophs bacteria are a plant’s surface inhabitant, especially at the leaf. They are known as Methylobacterium species. The antioxidant activity, phenolic compounds, and level of toxicity of this bacteria pigment have been studied. Recently, no previous research focused on the same bacterium found in Melicope lunu-ankenda (Gaertn.) T. G. Hartley, which is a component of the Malaysian ulam leaf. This study employed the 2,2-diphenyl-1-picrylhydrazyl (DPPH), ferric ion reducing antioxidant power (FRAP), and 2,2’-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging assays, along with total phenolic content determination to assess the antioxidant activities of the methanolic and ethanolic pigment extract. Additionally, the consumption safety level of the pigment extract used brine shrimp lethality assay. From these findings, ethanolic pigment extract has a higher antioxidant capacity than methanolic extract. The DPPH half-maximal inhibitory concentration (IC50) value of methanolic pigment extract is higher than ethanolic extract (0.72 ± 0.04 mg/ml), but the IC50 value is vice versa for ABTS (4.59 ± 2.17 mg/ml). Furthermore, ethanolic extracts have a high FRAP assay value (1.09 ± 0.19 mg/mg of trolox equivalent at 0.78 mg/ml sample) and phenolic content (1.39 ± 0.07 mg/mg of gallic acid equivalent at 0.78 mg/ml sample) compared to methanolic pigment extracts. Fortunately, the methanolic and ethanolic pigment extract’s lethal concentration values (4.52 and 9.94 mg/ml) are considered safe for food application since their toxicity level is higher than 1 mg/ml.","PeriodicalId":19890,"journal":{"name":"Pertanika Journal of Tropical Agricultural Science","volume":"138 1","pages":""},"PeriodicalIF":0.6000,"publicationDate":"2023-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Determination of Antioxidant Activity, Phenolic Compounds, and Toxicity of Methanolic and Ethanolic Extracts of Pink Pigmented Facultative Methylotrophs (PPFM) Bacteria Pigment\",\"authors\":\"Nur Isti’anah Ramli, F. Abas, I. S. Ismail, Y. Rukayadi, S. Md Nor\",\"doi\":\"10.47836/pjtas.46.4.21\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Pink-pigmented facultative methylotrophs bacteria are a plant’s surface inhabitant, especially at the leaf. They are known as Methylobacterium species. The antioxidant activity, phenolic compounds, and level of toxicity of this bacteria pigment have been studied. Recently, no previous research focused on the same bacterium found in Melicope lunu-ankenda (Gaertn.) T. G. Hartley, which is a component of the Malaysian ulam leaf. This study employed the 2,2-diphenyl-1-picrylhydrazyl (DPPH), ferric ion reducing antioxidant power (FRAP), and 2,2’-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging assays, along with total phenolic content determination to assess the antioxidant activities of the methanolic and ethanolic pigment extract. Additionally, the consumption safety level of the pigment extract used brine shrimp lethality assay. From these findings, ethanolic pigment extract has a higher antioxidant capacity than methanolic extract. The DPPH half-maximal inhibitory concentration (IC50) value of methanolic pigment extract is higher than ethanolic extract (0.72 ± 0.04 mg/ml), but the IC50 value is vice versa for ABTS (4.59 ± 2.17 mg/ml). Furthermore, ethanolic extracts have a high FRAP assay value (1.09 ± 0.19 mg/mg of trolox equivalent at 0.78 mg/ml sample) and phenolic content (1.39 ± 0.07 mg/mg of gallic acid equivalent at 0.78 mg/ml sample) compared to methanolic pigment extracts. Fortunately, the methanolic and ethanolic pigment extract’s lethal concentration values (4.52 and 9.94 mg/ml) are considered safe for food application since their toxicity level is higher than 1 mg/ml.\",\"PeriodicalId\":19890,\"journal\":{\"name\":\"Pertanika Journal of Tropical Agricultural Science\",\"volume\":\"138 1\",\"pages\":\"\"},\"PeriodicalIF\":0.6000,\"publicationDate\":\"2023-11-27\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Pertanika Journal of Tropical Agricultural Science\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.47836/pjtas.46.4.21\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"AGRICULTURE, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Pertanika Journal of Tropical Agricultural Science","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.47836/pjtas.46.4.21","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"AGRICULTURE, MULTIDISCIPLINARY","Score":null,"Total":0}
引用次数: 0

摘要

粉红色的兼性甲基营养细菌是植物表面的居民,尤其是在叶片上。它们被称为甲基细菌(Methylobacterium)。人们已经对这种细菌色素的抗氧化活性、酚类化合物和毒性水平进行了研究。最近,以前的研究没有关注在马来西亚乌兰树叶成分 Melicope lunu-ankenda (Gaertn.) T. G. Hartley 中发现的相同细菌。本研究采用了 2,2-二苯基-1-苦基肼(DPPH)、铁离子还原抗氧化力(FRAP)和 2,2'-偶氮-双(3-乙基苯并噻唑啉-6-磺酸)(ABTS)自由基清除试验,以及总酚含量测定来评估甲醇和乙醇色素提取物的抗氧化活性。此外,还使用盐水虾致死试验来评估色素提取物的食用安全水平。结果表明,乙醇色素提取物的抗氧化能力高于甲醇提取物。甲醇色素提取物的 DPPH 半最大抑制浓度(IC50)值(0.72 ± 0.04 mg/ml)高于乙醇提取物,但 ABTS 的 IC50 值(4.59 ± 2.17 mg/ml)则相反。此外,与甲醇色素提取物相比,乙醇提取物具有较高的 FRAP 检测值(1.09 ± 0.19 mg/mg trolox 当量,0.78 mg/ml 样品)和酚含量(1.39 ± 0.07 mg/mg 没食子酸当量,0.78 mg/ml 样品)。幸运的是,甲醇和乙醇色素提取物的致死浓度值(4.52 和 9.94 毫克/毫升)被认为可安全用于食品,因为它们的毒性水平高于 1 毫克/毫升。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
Determination of Antioxidant Activity, Phenolic Compounds, and Toxicity of Methanolic and Ethanolic Extracts of Pink Pigmented Facultative Methylotrophs (PPFM) Bacteria Pigment
Pink-pigmented facultative methylotrophs bacteria are a plant’s surface inhabitant, especially at the leaf. They are known as Methylobacterium species. The antioxidant activity, phenolic compounds, and level of toxicity of this bacteria pigment have been studied. Recently, no previous research focused on the same bacterium found in Melicope lunu-ankenda (Gaertn.) T. G. Hartley, which is a component of the Malaysian ulam leaf. This study employed the 2,2-diphenyl-1-picrylhydrazyl (DPPH), ferric ion reducing antioxidant power (FRAP), and 2,2’-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging assays, along with total phenolic content determination to assess the antioxidant activities of the methanolic and ethanolic pigment extract. Additionally, the consumption safety level of the pigment extract used brine shrimp lethality assay. From these findings, ethanolic pigment extract has a higher antioxidant capacity than methanolic extract. The DPPH half-maximal inhibitory concentration (IC50) value of methanolic pigment extract is higher than ethanolic extract (0.72 ± 0.04 mg/ml), but the IC50 value is vice versa for ABTS (4.59 ± 2.17 mg/ml). Furthermore, ethanolic extracts have a high FRAP assay value (1.09 ± 0.19 mg/mg of trolox equivalent at 0.78 mg/ml sample) and phenolic content (1.39 ± 0.07 mg/mg of gallic acid equivalent at 0.78 mg/ml sample) compared to methanolic pigment extracts. Fortunately, the methanolic and ethanolic pigment extract’s lethal concentration values (4.52 and 9.94 mg/ml) are considered safe for food application since their toxicity level is higher than 1 mg/ml.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Pertanika Journal of Tropical Agricultural Science
Pertanika Journal of Tropical Agricultural Science Agricultural and Biological Sciences-Agronomy and Crop Science
CiteScore
1.10
自引率
16.70%
发文量
64
期刊最新文献
Ultrasound and Steam Explosion Treatments on the Quantity and Molecular Size of Soluble Fibre Obtained from Un-purified and Purified Rice Bran Diversity of Wild Gingers (Zingiberaceae) in Southern Peninsular Malaysia: Panti Forest Reserve and Labis Forest Reserve Antidiabetic Activity of Bengkuang (Pachyrhizus erosus) Extracts in Diabetes Mellitus-induced Rats Performance of Climbing Perch (Anabas testudineus) and Bok Choy (Brassica chinensis) in Aquaponics Systems Using Nutrient Film Technique in Indonesian Small-scale Livestock Determination of Antioxidant Activity, Phenolic Compounds, and Toxicity of Methanolic and Ethanolic Extracts of Pink Pigmented Facultative Methylotrophs (PPFM) Bacteria Pigment
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1