利用基于 COI 的 DNA 条形码鉴定海竹(Isis hippuris)的物种

IF 0.6 Q3 AGRICULTURE, MULTIDISCIPLINARY Pertanika Journal of Tropical Agricultural Science Pub Date : 2023-11-17 DOI:10.47836/pjtas.46.4.17
La Ode Alirman Afu, Anis Chamidah, U. Yanuhar, Maftuch Maftuch
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引用次数: 0

摘要

海洋生态系统和生物多样性的保护和有效管理需要准确的物种鉴定。本研究利用 DNA 条形码技术对海竹(Isis hippuris)标本进行分类,该技术因其快速、准确和客观而得到广泛认可。本研究利用细胞色素 c 氧化酶亚单位 I(COI)基因分析进行物种鉴定。从苏拉威西岛东南部南科纳韦的 Tanjung Tiram 水域的两个站点(珊瑚区和海草区)采集了 Isis hippuris。从 I. hippuris 的基部、主茎和侧茎提取基因组 DNA。利用聚合酶链反应(PCR),使用 HCO2198 和 LCO1490 引物扩增 I. hippuris 的 mtDNA。根据 COI 基因选择质量最高的 PCR 产物进行测序分析。研究表明,COI 基因分析只能在 I. hippuris 的基部和主茎上进行。侧茎上的珊瑚和海草-珊瑚区域的样本由于浓度和纯度值较低,有可能导致 DNA 测序失败,因此没有进行进一步分析。I. hippuris的每个部分可能都有独特的遗传差异。这项研究强调了 DNA 测序在为每个物种提供独特遗传指纹方面的优势,从而能够准确识别物种。这项研究为使用 DNA 条形码进行海竹物种鉴定提供了启示。
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Species Identification of Sea Bamboo (Isis hippuris) using COI-based DNA Barcoding
Conservation and effective management of marine ecosystems and biodiversity requires accurate species identification. This study classifies sea bamboo (Isis hippuris) specimens using DNA barcoding, a technique widely recognized for its speed, accuracy, and objectivity. This study examines the cytochrome c oxidase subunit I (COI) gene analysis for species identification. Isis hippuris was collected from two stations (coral and seagrass areas) of Tanjung Tiram Waters, South Konawe, Southeast Sulawesi. Genomic DNA was extracted from the base, main, and lateral stem of I. hippuris. Polymerase chain reaction (PCR) was used to amplify the mtDNA of I. hippuris with HCO2198 and LCO1490 primers. The highest quality PCR product based on the COI gene was chosen for sequencing analysis. The study revealed that COI gene analysis could only be performed on the base and main stem of the I. hippuris. Samples from coral and seagrass-coral areas on lateral stems were not further analyzed due to low concentration and purity values, which could potentially fail DNA sequencing. Each part of I. hippuris may have unique genetic differences. This study highlights the advantages of DNA sequencing in providing a unique genetic fingerprint for each species, enabling accurate species identification. This research provides insight into using DNA barcoding for sea bamboo species identification.
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来源期刊
Pertanika Journal of Tropical Agricultural Science
Pertanika Journal of Tropical Agricultural Science Agricultural and Biological Sciences-Agronomy and Crop Science
CiteScore
1.10
自引率
16.70%
发文量
64
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