Hsa-Circ-0002360通过miRNA-511-5p调控特异性E3泛素蛋白连接酶2通路促进非小细胞肺癌细胞的发育

IF 2.9 4区 医学 Q1 Medicine Journal of biomedical nanotechnology Pub Date : 2023-11-01 DOI:10.1166/jbn.2023.3700
Hong Li, Fei Gao, Tian Yang, Sifang Feng, Qian Ning, Ya Liu, Tian-jun Chen
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引用次数: 0

摘要

肺癌是癌症患者最常见的癌症相关死亡原因之一,环状 RNA 在非小细胞肺癌中的具体作用仍有待进一步探索。本研究使用慢病毒表达载体 shcirc0002360、miRNA-511-5p mimics 和重组蛋白 SMURF2,探讨了 Hsa-Circ-0002360 的作用以及参与非小细胞肺癌细胞发展的相关途径。通过流式细胞术、伤口愈合、RT-qPCR和Western blot比较了细胞增殖、迁移和凋亡的功能。我们发现,circRNA 0002360敲除能显著抑制A549细胞的功能。进一步过表达 miR-511-5p 后,细胞的迁移功能、增殖功能和细胞活力明显降低,细胞凋亡率增加,而加入 SMURF2 后则恰恰相反。Smurf2通过调控肿瘤细胞的迁移、扩散和凋亡来促进非小细胞肺癌细胞的发展,而这一过程也受到Hsa-cic-000236和miR-511-5p的调控。
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Hsa-Circ-0002360 Promotes the Development of Non-Small Cell Lung Cancer Cells by Regulating Specific E3 Ubiquitin Protein Ligase 2 Pathway Through miRNA-511-5p
Lung cancer is one of the most common causes of cancer-related deaths for cancer patients, the specific role of cyclic RNA in non-small cell lung cancer still needs further exploration. The effect of Hsa-Circ-0002360 and the relative pathway participated in the development of non-small cell lung cancer cells, lentivirus expression vector shcirc0002360, miRNA-511-5p mimics and recombinant protein SMURF2 were used in this study. The function of cell proliferation, migration and apoptosis were compared by flow cytometry, wound healing, RT-qPCR and western blot. We found circRNA 0002360 knockout significantly inhibited A549 cell functions. After further overexpression of miR-511-5p, the migration function, proliferation function, and cell viability of cells are significantly decreased while apoptosis rate increased, and quite the opposite after adding SMURF2. Smurf2 promotes the development of non-small cell lung cancer cells by regulating tumor cell migration, dissemination, and apoptosis, and this process is also regulated by Hsa-cic-000236 and miR-511-5p.
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CiteScore
4.30
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17.20%
发文量
145
审稿时长
2.3 months
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