Jie Zhang, Zhihui Sheng, Hao Zhang, Wenwen Qi, Tao Jia
{"title":"槲皮素通过 miR-301b-3p/Phosphatase 和 Tensin Homolog Axis 调节抑制喉癌细胞的增殖和迁移","authors":"Jie Zhang, Zhihui Sheng, Hao Zhang, Wenwen Qi, Tao Jia","doi":"10.1166/jbn.2023.3703","DOIUrl":null,"url":null,"abstract":"In this study, we investigated the role of miR-301b-3p in promoting tumor cell proliferation and metastasis and explored the anti-cancer effects of quercetin in laryngocarcinoma cells. Through Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) annotation analyses, we examined the effects of miR-301b-3p and PTEN on potential target genes. We measured laryngocarcinoma cell activity and apoptosis using CCK8 and flow cytometry, respectively, and assessed migration and invasion through transwell assay. qRT-PCR was used to determine the levels of miR-301b-3p and PTEN in laryngocarcinoma cells. Luciferase activity and western blot assays were employed to study the interaction between miR-301b-3p and PTEN. We found that miR-301b-3p was associated with various types of cancer, and pathways related to miR-301b-3p overlapped with those of PTEN. Quercetin effectively inhibited the proliferation, migration, and invasion of laryngocarcinoma cells, but these effects were reversed by miR-301b-3p overexpression. The level of miR-301b-3p was significantly increased in laryngocarcinoma cells, leading to down-regulation of PTEN protein and enhanced tumor cell activity. However, restoring PTEN alleviated the malignant growth caused by miR-301b-3p overexpression. Ultimately, quercetin exerted its inhibitory effects on proliferation, migration, and invasion by regulating the miR-301b-3p/PTEN axis in laryngocarcinoma cells. These findings highlight the potential of quercetin as a promising treatment option for laryngocarcinoma.","PeriodicalId":15260,"journal":{"name":"Journal of biomedical nanotechnology","volume":"46 1","pages":""},"PeriodicalIF":2.9000,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Quercetin Inhibits Proliferation and Migration via miR-301b-3p/Phosphatase and Tensin Homolog Axis Regulation in Laryngocarcinoma Cells\",\"authors\":\"Jie Zhang, Zhihui Sheng, Hao Zhang, Wenwen Qi, Tao Jia\",\"doi\":\"10.1166/jbn.2023.3703\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"In this study, we investigated the role of miR-301b-3p in promoting tumor cell proliferation and metastasis and explored the anti-cancer effects of quercetin in laryngocarcinoma cells. Through Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) annotation analyses, we examined the effects of miR-301b-3p and PTEN on potential target genes. We measured laryngocarcinoma cell activity and apoptosis using CCK8 and flow cytometry, respectively, and assessed migration and invasion through transwell assay. qRT-PCR was used to determine the levels of miR-301b-3p and PTEN in laryngocarcinoma cells. Luciferase activity and western blot assays were employed to study the interaction between miR-301b-3p and PTEN. We found that miR-301b-3p was associated with various types of cancer, and pathways related to miR-301b-3p overlapped with those of PTEN. Quercetin effectively inhibited the proliferation, migration, and invasion of laryngocarcinoma cells, but these effects were reversed by miR-301b-3p overexpression. The level of miR-301b-3p was significantly increased in laryngocarcinoma cells, leading to down-regulation of PTEN protein and enhanced tumor cell activity. However, restoring PTEN alleviated the malignant growth caused by miR-301b-3p overexpression. Ultimately, quercetin exerted its inhibitory effects on proliferation, migration, and invasion by regulating the miR-301b-3p/PTEN axis in laryngocarcinoma cells. These findings highlight the potential of quercetin as a promising treatment option for laryngocarcinoma.\",\"PeriodicalId\":15260,\"journal\":{\"name\":\"Journal of biomedical nanotechnology\",\"volume\":\"46 1\",\"pages\":\"\"},\"PeriodicalIF\":2.9000,\"publicationDate\":\"2023-11-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of biomedical nanotechnology\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://doi.org/10.1166/jbn.2023.3703\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"Medicine\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of biomedical nanotechnology","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1166/jbn.2023.3703","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"Medicine","Score":null,"Total":0}
Quercetin Inhibits Proliferation and Migration via miR-301b-3p/Phosphatase and Tensin Homolog Axis Regulation in Laryngocarcinoma Cells
In this study, we investigated the role of miR-301b-3p in promoting tumor cell proliferation and metastasis and explored the anti-cancer effects of quercetin in laryngocarcinoma cells. Through Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) annotation analyses, we examined the effects of miR-301b-3p and PTEN on potential target genes. We measured laryngocarcinoma cell activity and apoptosis using CCK8 and flow cytometry, respectively, and assessed migration and invasion through transwell assay. qRT-PCR was used to determine the levels of miR-301b-3p and PTEN in laryngocarcinoma cells. Luciferase activity and western blot assays were employed to study the interaction between miR-301b-3p and PTEN. We found that miR-301b-3p was associated with various types of cancer, and pathways related to miR-301b-3p overlapped with those of PTEN. Quercetin effectively inhibited the proliferation, migration, and invasion of laryngocarcinoma cells, but these effects were reversed by miR-301b-3p overexpression. The level of miR-301b-3p was significantly increased in laryngocarcinoma cells, leading to down-regulation of PTEN protein and enhanced tumor cell activity. However, restoring PTEN alleviated the malignant growth caused by miR-301b-3p overexpression. Ultimately, quercetin exerted its inhibitory effects on proliferation, migration, and invasion by regulating the miR-301b-3p/PTEN axis in laryngocarcinoma cells. These findings highlight the potential of quercetin as a promising treatment option for laryngocarcinoma.