槲皮素通过 miR-301b-3p/Phosphatase 和 Tensin Homolog Axis 调节抑制喉癌细胞的增殖和迁移

IF 2.9 4区 医学 Q1 Medicine Journal of biomedical nanotechnology Pub Date : 2023-11-01 DOI:10.1166/jbn.2023.3703
Jie Zhang, Zhihui Sheng, Hao Zhang, Wenwen Qi, Tao Jia
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引用次数: 0

摘要

在这项研究中,我们研究了 miR-301b-3p 在促进肿瘤细胞增殖和转移中的作用,并探讨了槲皮素在喉癌细胞中的抗癌作用。通过基因本体(GO)和京都基因组百科全书(KEGG)注释分析,我们研究了miR-301b-3p和PTEN对潜在靶基因的影响。我们使用 CCK8 和流式细胞术分别测量了喉癌细胞的活性和凋亡,并通过跨孔试验评估了迁移和侵袭。荧光素酶活性和 Western 印迹检测被用来研究 miR-301b-3p 和 PTEN 之间的相互作用。我们发现,miR-301b-3p 与多种癌症相关,而且与 miR-301b-3p 相关的通路与 PTEN 的通路重叠。槲皮素能有效抑制喉癌细胞的增殖、迁移和侵袭,但 miR-301b-3p 的过表达会逆转这些效应。喉癌细胞中 miR-301b-3p 的水平显著升高,导致 PTEN 蛋白下调,增强了肿瘤细胞的活性。然而,恢复 PTEN 可以缓解 miR-301b-3p 过表达引起的恶性生长。最终,槲皮素通过调节喉癌细胞的miR-301b-3p/PTEN轴,发挥了抑制增殖、迁移和侵袭的作用。这些发现凸显了槲皮素作为喉癌治疗选择的潜力。
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Quercetin Inhibits Proliferation and Migration via miR-301b-3p/Phosphatase and Tensin Homolog Axis Regulation in Laryngocarcinoma Cells
In this study, we investigated the role of miR-301b-3p in promoting tumor cell proliferation and metastasis and explored the anti-cancer effects of quercetin in laryngocarcinoma cells. Through Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) annotation analyses, we examined the effects of miR-301b-3p and PTEN on potential target genes. We measured laryngocarcinoma cell activity and apoptosis using CCK8 and flow cytometry, respectively, and assessed migration and invasion through transwell assay. qRT-PCR was used to determine the levels of miR-301b-3p and PTEN in laryngocarcinoma cells. Luciferase activity and western blot assays were employed to study the interaction between miR-301b-3p and PTEN. We found that miR-301b-3p was associated with various types of cancer, and pathways related to miR-301b-3p overlapped with those of PTEN. Quercetin effectively inhibited the proliferation, migration, and invasion of laryngocarcinoma cells, but these effects were reversed by miR-301b-3p overexpression. The level of miR-301b-3p was significantly increased in laryngocarcinoma cells, leading to down-regulation of PTEN protein and enhanced tumor cell activity. However, restoring PTEN alleviated the malignant growth caused by miR-301b-3p overexpression. Ultimately, quercetin exerted its inhibitory effects on proliferation, migration, and invasion by regulating the miR-301b-3p/PTEN axis in laryngocarcinoma cells. These findings highlight the potential of quercetin as a promising treatment option for laryngocarcinoma.
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CiteScore
4.30
自引率
17.20%
发文量
145
审稿时长
2.3 months
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