鉴定捕获的大鼠种类并检测钩端螺旋体细菌:在泗水丹戎霹雳港 Gapura Surya Nusantara 客运站进行的研究

Mas Adhi Hardian Utama, S. Suhartono, B. Budiyono
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The research results indicate Rattus norvegicus as the most dominant species, followed by Mus musculus and Rattus tanezumi. Out of a total of 19 captured rats, 89.5% were male, and 10.5% were female. RT-PCR testing for Leptospira bacteria in rat kidneys showed that only 3 rats (15.8%) tested positive, while 16 rats (84.2%) tested negative. Interestingly, rats identified as exposed to Leptospira bacteria were only found in the Rattus norvegicus species and were male. This highlights the potential role of species and gender in the spread of these bacteria. Increased surveillance and control measures are needed to address the issue of rats and the spread of Leptospira bacteria. The Rattus norvegicus species, particularly the males exposed to Leptospira bacteria, requires special attention. Abstrak: Leptospirosis ditularkan melalui air atau makanan terkontaminasi oleh urine hewan terinfeksi, dengan tikus sebagai potensi reservoir utama. 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引用次数: 0

摘要

钩端螺旋体病是通过受感染动物尿液污染的水或食物传播的,而老鼠是潜在的主要传播源。本研究旨在描述泗水丹戎霹雳 Gapura Surya Nusantara(GSN)港口码头老鼠的种类和性别,并分析老鼠体内存在的钩端螺旋体细菌。本研究采用描述性横断面设计。使用以咸鱼为诱饵的诱捕器捕获样本。捕获的大鼠肾脏在泗水环境健康和疾病控制技术中心(BBTKLPP)的病媒和疾病载体动物实验室装置中使用实时聚合酶链式反应(RT-PCR)进行检测。数据使用频率分布表进行分析。研究结果表明,野鼠(Rattus norvegicus)是最主要的鼠类,其次是麝鼠(Mus musculus)和豚鼠(Rattus tanezumi)。在捕获的 19 只老鼠中,89.5% 为雄性,10.5% 为雌性。大鼠肾脏中钩端螺旋体细菌的 RT-PCR 检测显示,只有 3 只大鼠(15.8%)的检测结果呈阳性,而 16 只大鼠(84.2%)的检测结果呈阴性。有趣的是,被确认接触过钩端螺旋体细菌的大鼠只属于鼠科动物,而且都是雄性。这凸显了物种和性别在这些细菌传播中的潜在作用。需要加强监测和控制措施,以解决老鼠和钩端螺旋体细菌传播的问题。需要特别关注鼠类,尤其是暴露于钩端螺旋体细菌的雄性鼠类。摘要钩端螺旋体病是通过受感染动物尿液污染的水或食物传播的,而老鼠是主要的潜在传播源。本研究旨在描述泗水丹戎霹雳港 Gapura Surya Nusantara(GSN)码头老鼠的种类和性别,并分析其中存在的钩端螺旋体细菌。本研究为描述性横断面研究。使用咸鱼诱饵诱捕器采集样本。在泗水环境卫生和疾病控制工程中心(BBTKLPP)的病媒和带病动物实验室装置中,使用实时聚合酶链式反应(RT-PCR)方法对捕获的老鼠的肾脏进行检测。数据使用频率分布表进行分析。结果显示,最主要的物种是无尾白老鼠,其次是麝香鼠(Mus musculus)和金丝鼠(Rattus tanezumi)。在捕获的 19 只老鼠中,89.5% 为雄性,10.5% 为雌性。大鼠肾脏中钩端螺旋体细菌的 RT-PCR 检测结果显示,只有 3 只大鼠(15.8%)呈阳性,16 只大鼠(84.2%)呈阴性。有趣的是,被确认接触过钩端螺旋体细菌的大鼠只属于鼠科动物,而且都是雄性。这凸显了物种和性别在细菌传播中的潜在作用。需要加强监测和控制,以解决老鼠问题和钩端螺旋体细菌的传播。雄性鼠类作为暴露于钩端螺旋体细菌的老鼠,需要特别关注。
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Identification of Captured Rat Species and Detection of Leptospira Bacteria: Study at the Gapura Surya Nusantara Passenger Terminal, Tanjung Perak Port, Surabaya
Leptospirosis is transmitted through water or food contaminated by the urine of infected animals, with rats as the potential main reservoir. This research aims to describe the species and gender and analyze the presence of Leptospira bacteria in rats at the Terminal Gapura Surya Nusantara (GSN) Port in Tanjung Perak, Surabaya. This study employed a descriptive cross-sectional design. Samples were captured using traps baited with salted fish. The captured rat kidneys were tested using Real-Time Polymerase Chain Reaction (RT-PCR) at the Vector and Disease Carrier Animal Laboratory Installation of the Center for Environmental Health and Disease Control Technology (BBTKLPP) in Surabaya. Data were analyzed using frequency distribution tables. The research results indicate Rattus norvegicus as the most dominant species, followed by Mus musculus and Rattus tanezumi. Out of a total of 19 captured rats, 89.5% were male, and 10.5% were female. RT-PCR testing for Leptospira bacteria in rat kidneys showed that only 3 rats (15.8%) tested positive, while 16 rats (84.2%) tested negative. Interestingly, rats identified as exposed to Leptospira bacteria were only found in the Rattus norvegicus species and were male. This highlights the potential role of species and gender in the spread of these bacteria. Increased surveillance and control measures are needed to address the issue of rats and the spread of Leptospira bacteria. The Rattus norvegicus species, particularly the males exposed to Leptospira bacteria, requires special attention. Abstrak: Leptospirosis ditularkan melalui air atau makanan terkontaminasi oleh urine hewan terinfeksi, dengan tikus sebagai potensi reservoir utama. Penelitian ini bertujuan untuk mendeskripsikan spesies dan jenis kelamin, serta menganalisis keberadaan bakteri Leptospira pada tikus di Terminal Gapura Surya Nusantara (GSN) Pelabuhan Tanjung Perak Surabaya. Penelitian ini adalah deskriptif cross-sectional. Sampel diambil dengan perangkap menggunakan umpan ikan asin. Ginjal tikus yang tertangkap diuji menggunakan metode Real Time Polymerase Chain Reaction (RT-PCR) di Instalasi Laboratorium Vektor dan Binatang Pembawa Penyakit Balai Besar Teknik Kesehatan Lingkungan dan Pengendalian Penyakit (BBTKLPP)  Surabaya. Data dianalisis menggunakan tabel distribusi frekuensi. Hasil penelitian menunjukkan Rattus norvegicus sebagai spesies paling dominan, diikuti oleh Mus musculus dan Rattus tanezumi. Dari total 19 tikus yang berhasil ditangkap, sebanyak 89,5% adalah jantan dan 10,5% betina. Hasil uji RT-PCR untuk bakteri Leptospira pada ginjal tikus menunjukkan hanya 3 tikus (15,8%) positif, sedangkan 16 tikus (84,2%) negatif. Menariknya, tikus yang teridentifikasi terpapar oleh bakteri Leptospira hanya ditemukan pada jenis Rattus norvegicus dan berjenis kelamin jantan. Hal ini menyoroti peran potensial spesies dan jenis kelamin dalam penyebaran bakteri tersebut. Peningkatan pengawasan dan pengendalian dibutuhkan untuk menanggulangi masalah tikus dan penyebaran bakteri Leptospira. Spesies Rattus norvegicus yang berjenis kelamin jantan, sebagai tikus yang terpapar bakteri Leptospira, memerlukan perhatian khusus.
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