马格兰萝卜多糖的分离与研究

Y. Oshchepkova, S. I. Salikhov
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摘要

本研究的目的是分离和研究乌兹别克斯坦共和国栽培的十字花科马吉兰萝卜种子中多糖的理化特性。 在提取蛋白质和多酚之后,通过播种玛吉兰连续提取,首次从萝卜种子中分离出多糖的水溶性部分。采用阴离子交换色谱法和凝胶过滤法获得均匀的多糖。多糖部分经离子交换色谱分离,在 Sephadex G-100 柱上纯化。结果得到两种多糖 RSP1 和 RSP2。确定了分离多糖的单糖组成。单糖残基分析表明,RSP1 多糖的单糖组成如下:鼠李糖-0.7%、核糖-4.7%、阿拉伯糖-55.6%、木糖-1.5%、甘露糖-3.7%、葡萄糖-5.1%和半乳糖-28.7%。RSP2 多糖的组成以单糖为代表:核糖 - 2.8%,阿拉伯糖 - 9.4%,甘露糖 - 0.9%,葡萄糖 - 64.9%,半乳糖 - 18.4%。从所得结果可以看出,RSP1 多糖主要由阿拉伯糖(55.6%)和半乳糖(28.7%)残基组成,属于阿拉伯半乳聚糖类型,而 RSP2 多糖主要由葡萄糖残基(64.9%)和半乳糖(18.4%)组成。
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ISOLATION AND STUDY OF POLYSACCHARIDES OF MARGILAN RADISH RAPHANUS SATIVUS
The purpose of this research is to isolate and study the physicochemical characteristics of polysaccharides in the seeds of the Margilan radish of the Brassicaceae family cultivated in the Republic of Uzbekistan. For the first time, a water-soluble fraction of polysaccharides was isolated from radish seeds by sowing Margilan successive extraction after the extraction of proteins and polyphenols. Anion exchange chromatography and gel filtration were used to obtain homogeneous polysaccharides. The polysaccharide fraction was separated by ion-exchange chromatography, purified on a Sephadex G-100 column. As a result, two polysaccharides RSP1 and RSP2 were obtained. The monosaccharide composition of the isolated polysaccharides was established. Analysis of monosaccharide residues showed that the composition of the RSP1 polysaccharide is represented by monosaccharides in the following composition: rhamnose - 0.7%, ribose - 4.7%, arabinose - 55.6%, xylose - 1.5%, mannose - 3.7%, glucose - 5.1% and galactose - 28.7%. The composition of the RSP2 polysaccharide is represented by monosaccharides: ribose - 2.8%, arabinose - 9.4%, mannose - 0.9%, glucose - 64.9% and galactose - 18.4%. From the obtained results, it can be seen that the RSP1 polysaccharide consists mainly of arabinose (55.6%) and galactose (28.7%) residues and belongs to the type of arabinogalactans, and the RSP2 polysaccharide mainly consists of glucose residues (64.9%) and galactose (18.4%).
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