鹿角苏木(Rhus typhina L.)果实的有机酸

Ivan Viktorovich Popov, Viktoriya Nodar'yevna Leonova, Ol'ga Ivanovna Popova
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摘要

无患子科的鹿角苏木(Rhus typhina L.)是一种落叶乔木,原产于北美洲,在俄罗斯有栽培。这项工作的目的是测定鹿角刺槐果实中的有机酸。研究对象是新鲜、干燥和越冬(去年)的鹿角刺槐果实。为了对抗坏血酸和有机酸进行定性分析,提取物通过三种方式获得:在带有反向制冷机的水浴烧瓶中提取、浸泡方式提取和煎煮方式提取。有机酸的测定采用定性反应、薄层色谱法和高效液相色谱法。通过薄层色谱法,从三个原料样品的提取物中鉴定出草酸、酒石酸、柠檬酸、抗坏血酸和苹果酸。高效液相色谱法证实了五种有机酸的存在。有机酸总和的含量为:鲜果 - 6.52%,干果 - 6.26%,越冬果 - 6.35%。抗坏血酸的含量分别为 0.78%、0.75% 和 0.76%。有机酸和抗坏血酸总和的高含量使我们可以将鹿角苏木的果实视为生物活性化合物的宝贵来源。
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ORGANIC ACIDS OF THE FRUITS OF STAGHORN SUMAC (RHUS TYPHINA L.)
Staghorn sumac (Rhus typhina L.) of the Anacardiaceae family is a deciduous tree originating from North America, cultivated in Russia. The aim of the work was to determine the organic acids in the fruits of staghorn sumac. The object of the study was the fruits of staghorn sumac, fresh, dried and overwintered (last year). For qualitative analysis of ascorbic acid and organic acids, extracts were obtained in three ways: in a flask in a water bath with a reverse refrigerator, in infusion mode, in decoction mode. The determination of organic acids was carried out using qualitative reactions, as well as by thin-layer chromatography and high-performance liquid chromatography. By the method of thin-layer chromatography in extracts from three samples of raw materials were identified oxalic, tartaric, citric, ascorbic and malic acids. The method of high-performance liquid chromatography confirmed the presence of five organic acids. The content of the sum of organic acids was: in fresh fruits – 6.52%, in dry fruits – 6.26%, in overwintered fruits – 6.35%. The content of ascorbic acid is 0.78%, 0.75% and 0.76%, respectively. The high content of the sum of organic acids and ascorbic acid allows us to consider the fruits of staghorn sumac as a valuable source of biologically active compounds.
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