Saluang Belum(Lavanga sarmentosa)根煎剂对小鼠曲细精管组织学图像中精子数量的影响

Silvani Permatasari, Hairunnida Rahmatina, Ratna Widayati, I. G. Eka A
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摘要

加里曼丹的达雅克(Dayak)部落因其传统药用植物 Saluang Belum(Lavanga sarmentosa)的根而闻名,这种植物具有增强体力和性兴奋的特性,并被用作提高男性生育能力的替代药物。L.sarmentosa的食用方法是饮用植物根部的沸水。本研究的目的是确定灌注 Saluang Belum(L.sarmentosa)根对增加小鼠(Mus musculus)精小管组织学所见精子数量的影响。采用灌注法提取根部,每组剂量不同,分别为 200 毫克/千克体重、400 毫克/千克体重和 600 毫克/千克体重,以及阴性对照组(水样),对小鼠进行为期 15 天的试验。第 16 天,取小鼠右侧睾丸,经苏木精-伊红染色后进行组织学制备,并在显微镜下以 400 倍放大镜观察五个视野。输精管中含有的化合物有生物碱、皂苷、三萜类、黄酮类、酚类和类固醇。小鼠精小管组织学中的精子数量在 200 毫克/千克体重、400 毫克/千克体重和 600 毫克/千克体重的剂量下明显增加。 结论灌注L.sarmentosa根可以增加小鼠精小管组织学中的精子数量。
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Pengaruh Rebusan Akar Saluang Belum (Lavanga sarmentosa) Terhadap Jumlah Spermatid pada Gambaran Histologis Tubulus Seminiferus Mencit
The Dayak tribe of Kalimantan is known as the root of Saluang Belum (Lavanga sarmentosa) a traditional medicinal plant that has properties to increase stamina, and sexual arousal and is used as an alternative medicine to increase male fertility. L.sarmentosa is consumed by drinking the boiled water of the plant's roots. The aim of this study is to determine the effect infusion roots of Saluang Belum (L.sarmentosa) in increasing the spermatids as seen in the tubulus seminiferus histological of mice (Mus musculus). The root will be extracted by the infusion method with different doses for each group, namely 200 mg/KgBB, 400 mg/KgBB, and 600 mg/KgBB, and negative controls (aquades) which was carried out for 15 days to mice. On the day 16, the right testis was taken and histological preparations were made with hematoxylin-eosin staining and observed under a microscope at 400x magnification in five fields of view. Compounds contained in the infusion of L.sarmentosa are alkaloids, saponins, triterpenoids, flavonoids, phenolics, and steroids. The number of spermatids in the tubulus seminiferus histological of mice increased statistically significantly from the doses of 200 mg/KgBB, 400 mg/KgBB, and 600 mg/KBBg.  Conclusion: Infusion of the roots L.sarmentosa can increase the number of spermatids seen in the tubulus seminiferus histological of mice.
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