N. Yu. Gerasimov, O. V. Nevrova, I. V. Zhigacheva, I. P. Generozova, A. N. Goloshchapov
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引用次数: 0
摘要
研究了抗氧化剂、植物多酚白藜芦醇和一氧化氮供体硫亚硝基铁与硫代硫酸盐的络合物 Na2[Fe2(S2O3)2(NO)4]2 - 4H2O （TNIC-thio）的相互作用，以及它们在体外对豌豆幼苗外胚叶线粒体的联合作用。浓度为 10-6 M 的白藜芦醇的抗氧化活性部分补偿了高浓度 TNIC-thio 的毒性作用，这很可能是由于高浓度一氧化氮供体的促氧化特性的体现。在 10-8 M TNIC-thio 存在下，浓度为 10-6 和 10-8 M 的白藜芦醇对从豌豆幼苗外胚叶分离的线粒体膜的影响导致膜脂质过氧化调节系统受到破坏，从而引起抗氧化应激。剂量为 2 × 10-5 M 的白藜芦醇具有双重作用，实际上不会影响线粒体膜的结构状态。
The Effect of the Antioxidant System “Resveratrol – Iron Sulfonitrosyl Complex Sodium-μ2-Dithiosulphate-Tetranitrosyl Diferrate Tetrahydrate” on the Mitochondria of Pea Germ Epicotyls in vitro
The interaction of antioxidants, plant polyphenol resveratrol and nitric oxide donor, sulfur nitrosyl iron complex with thiosulfate Na2[Fe2(S2O3)2(NO)4]2 · 4H2O (TNIC-thio), and their combined action on the mitochondria of epicotyls of pea seedlings in vitro were studied. The antioxidant activity of resveratrol at a concentration of 10–6 M partially compensated for the toxic effect of TNIC-thio at high concentrations, which was most likely due to the manifestation of the pro-oxidant properties of high concentrations of the nitric oxide donor. The effect of resveratrol at concentrations of 10–6 and 10–8 M on the membranes of mitochondria isolated from the epicotyls of pea seedlings in the presence of 10–8 M TNIC-thio led to a violation of the regulation system of lipid peroxidation of membranes, which caused antioxidant stress. The effect of resveratrol at a dose of 2 × 10–5 M was of a dual nature and practically did not affect the structural state of mitochondrial membranes.
BiophysicsBiochemistry, Genetics and Molecular Biology-Biophysics
CiteScore
1.20
自引率
0.00%
发文量
67
期刊介绍：
Biophysics is a multidisciplinary international peer reviewed journal that covers a wide scope of problems related to the main physical mechanisms of processes taking place at different organization levels in biosystems. It includes structure and dynamics of macromolecules, cells and tissues; the influence of environment; energy transformation and transfer; thermodynamics; biological motility; population dynamics and cell differentiation modeling; biomechanics and tissue rheology; nonlinear phenomena, mathematical and cybernetics modeling of complex systems; and computational biology. The journal publishes short communications devoted and review articles.
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