Tianqi Zhao, Ping Tang, Chonglan Liu, Rubin Zuo, Shunyu Su, Yuanyuan Zhong, Yongjie Li and Jing Yang*,
{"title":"多指标方法揭示 OsPIL1 是促进水稻生长、谷粒发育和抗瘟性的调控因子。","authors":"Tianqi Zhao, Ping Tang, Chonglan Liu, Rubin Zuo, Shunyu Su, Yuanyuan Zhong, Yongjie Li and Jing Yang*, ","doi":"10.1021/acs.jafc.3c07330","DOIUrl":null,"url":null,"abstract":"<p >Rice (<i>Oryza sativa</i>) is a crucial crop, achieving high yield concurrent pathogen resistance remains a challenge. Transcription factors play roles in growth and abiotic tolerance. However, rice phytochrome-interacting factor-like 1 (<i>OsPIL1</i>) in pathogen resistance and agronomic traits remains unexplored. We generated <i>OsPIL1</i> overexpressing (<i>OsPIL1</i> OE) rice lines and evaluated their impact on growth, grain development, and resistance to <i>Magnaporthe oryzae</i>. Multiomics analysis (RNA-seq, metabolomics, and CUT&Tag) and RT-qPCR validated <i>OsPIL1</i> target genes and key metabolites. In the results, <i>OsPIL1</i> OE rice lines exhibited robust growth, longer grains, and enhanced resistance to <i>M. oryzae</i> without compromising growth. Integrative multiomics analysis revealed a coordinated regulatory network centered on <i>OsPIL1</i>, explaining these desirable traits. <i>OsPIL1</i> likely acts as a positive regulator, targeting transcriptional elements or specific genes with direct functions in several biological programs. In particular, a range of key signaling genes (phosphatases, kinases, plant hormone genes, transcription factors), and metabolites (linolenic acid, vitamin E, trigonelline, <span>d</span>-glucose, serotonin, choline, genistein, riboflavin) contributed to enhanced rice growth, grain size, pathogen resistance, or a combination of these traits. These findings highlight <i>OsPIL1</i>’s regulatory role in promoting important traits and provide insights into potential strategies for rice breeding.</p>","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":null,"pages":null},"PeriodicalIF":5.7000,"publicationDate":"2024-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Multi-Omics Approach Reveals OsPIL1 as a Regulator Promotes Rice Growth, Grain Development, and Blast Resistance\",\"authors\":\"Tianqi Zhao, Ping Tang, Chonglan Liu, Rubin Zuo, Shunyu Su, Yuanyuan Zhong, Yongjie Li and Jing Yang*, \",\"doi\":\"10.1021/acs.jafc.3c07330\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p >Rice (<i>Oryza sativa</i>) is a crucial crop, achieving high yield concurrent pathogen resistance remains a challenge. Transcription factors play roles in growth and abiotic tolerance. However, rice phytochrome-interacting factor-like 1 (<i>OsPIL1</i>) in pathogen resistance and agronomic traits remains unexplored. We generated <i>OsPIL1</i> overexpressing (<i>OsPIL1</i> OE) rice lines and evaluated their impact on growth, grain development, and resistance to <i>Magnaporthe oryzae</i>. Multiomics analysis (RNA-seq, metabolomics, and CUT&Tag) and RT-qPCR validated <i>OsPIL1</i> target genes and key metabolites. In the results, <i>OsPIL1</i> OE rice lines exhibited robust growth, longer grains, and enhanced resistance to <i>M. oryzae</i> without compromising growth. Integrative multiomics analysis revealed a coordinated regulatory network centered on <i>OsPIL1</i>, explaining these desirable traits. <i>OsPIL1</i> likely acts as a positive regulator, targeting transcriptional elements or specific genes with direct functions in several biological programs. In particular, a range of key signaling genes (phosphatases, kinases, plant hormone genes, transcription factors), and metabolites (linolenic acid, vitamin E, trigonelline, <span>d</span>-glucose, serotonin, choline, genistein, riboflavin) contributed to enhanced rice growth, grain size, pathogen resistance, or a combination of these traits. These findings highlight <i>OsPIL1</i>’s regulatory role in promoting important traits and provide insights into potential strategies for rice breeding.</p>\",\"PeriodicalId\":41,\"journal\":{\"name\":\"Journal of Agricultural and Food Chemistry\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":5.7000,\"publicationDate\":\"2024-01-08\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Agricultural and Food Chemistry\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://pubs.acs.org/doi/10.1021/acs.jafc.3c07330\",\"RegionNum\":1,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"AGRICULTURE, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Agricultural and Food Chemistry","FirstCategoryId":"97","ListUrlMain":"https://pubs.acs.org/doi/10.1021/acs.jafc.3c07330","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRICULTURE, MULTIDISCIPLINARY","Score":null,"Total":0}
Multi-Omics Approach Reveals OsPIL1 as a Regulator Promotes Rice Growth, Grain Development, and Blast Resistance
Rice (Oryza sativa) is a crucial crop, achieving high yield concurrent pathogen resistance remains a challenge. Transcription factors play roles in growth and abiotic tolerance. However, rice phytochrome-interacting factor-like 1 (OsPIL1) in pathogen resistance and agronomic traits remains unexplored. We generated OsPIL1 overexpressing (OsPIL1 OE) rice lines and evaluated their impact on growth, grain development, and resistance to Magnaporthe oryzae. Multiomics analysis (RNA-seq, metabolomics, and CUT&Tag) and RT-qPCR validated OsPIL1 target genes and key metabolites. In the results, OsPIL1 OE rice lines exhibited robust growth, longer grains, and enhanced resistance to M. oryzae without compromising growth. Integrative multiomics analysis revealed a coordinated regulatory network centered on OsPIL1, explaining these desirable traits. OsPIL1 likely acts as a positive regulator, targeting transcriptional elements or specific genes with direct functions in several biological programs. In particular, a range of key signaling genes (phosphatases, kinases, plant hormone genes, transcription factors), and metabolites (linolenic acid, vitamin E, trigonelline, d-glucose, serotonin, choline, genistein, riboflavin) contributed to enhanced rice growth, grain size, pathogen resistance, or a combination of these traits. These findings highlight OsPIL1’s regulatory role in promoting important traits and provide insights into potential strategies for rice breeding.
期刊介绍:
The Journal of Agricultural and Food Chemistry publishes high-quality, cutting edge original research representing complete studies and research advances dealing with the chemistry and biochemistry of agriculture and food. The Journal also encourages papers with chemistry and/or biochemistry as a major component combined with biological/sensory/nutritional/toxicological evaluation related to agriculture and/or food.