奶牛产后卵泡发育恢复期间生长激素及其受体表达的评估

IF 2.5 3区 生物学 Q3 REPRODUCTIVE BIOLOGY Reproductive biology Pub Date : 2024-01-09 DOI:10.1016/j.repbio.2023.100848
L.I. Durante , E. Angeli , L. Etchevers , U.S. Notaro , F.M. Rodríguez , H.H. Ortega , B.E. Marelli
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引用次数: 0

摘要

生长激素是奶牛泌乳期代谢适应和最佳繁殖功能的关键内分泌因子。本研究旨在分析产后卵泡恢复发育过程中 GH 及其受体(GHR)在卵泡中的表达以及代谢生物标志物,并分析排卵前卵泡中 GH 和 GHR 的免疫定位和蛋白表达。根据产后天数(PPD)将36头奶牛分为两组:产后天数较少的奶牛(FPPD组,n = 15)和产后天数较多的奶牛(MPPD组,n = 22)。在第二次分析中,根据产犊季节(S)将相同的奶牛重新分组,分为秋季产犊奶牛组(n = 20)和冬季产犊奶牛组(n = 17)。在PP期间,在两个时间点(T)采集血液和卵泡吸液:第一个优势卵泡形成时(T1,第9±2天)和排卵前卵泡形成时(T2,第45±2天)。此外,六头奶牛在发情期被切除卵巢,并获得卵巢组织切片。在 PP 期采样的卵泡颗粒细胞中检测到生长激素 mRNA。在GHR mRNA方面观察到PPD × T的交互作用,在T1时,FPPD奶牛的GHR mRNA高于MPPD奶牛。代谢生物标志物和生殖激素在 PPD、T 和 S 之间显示出差异或相互作用,这取决于具体情况。此外,排卵前卵泡的颗粒细胞和卵巢间质细胞中也出现了 GH 和 GHR 的免疫定位。这些结果证实了 GH 和 GHR 在奶牛成熟卵泡中的表达,并表明 GHR 表达越多与产后卵泡发育恢复越早之间可能存在关联。
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Evaluation of the expression of growth hormone and its receptor during the resumption of postpartum ovarian follicle development in dairy cows

Growth hormone is a key endocrine factor for metabolic adaptations to lactation and optimal reproductive function of the dairy cow. This study aimed to analyze the expression of GH and its receptor (GHR) in ovarian follicles, along with metabolic biomarkers, during the resumption of the postpartum follicular development, and to analyze the immunolocalization and protein expression of GH and GHR in preovulatory follicles. Thirty-six dairy cows were grouped according to the postpartum days (PPD) until the establishment of the first dominant follicle in: cows that established their first dominant follicle at fewer postpartum days (FPPD group; n = 15) and cows that established their first dominant follicle at more postpartum days (MPPD group; n = 22). For a second analysis, the same cows were regrouped according to the calving season (S), into cows calving in autumn (n = 20) and cows calving in winter (n = 17). During the PP, blood and follicular aspirates were obtained at two timepoints (T): when the first dominant follicle was established (T1, day 9 ± 2), and when the preovulatory follicle was established (T2, day 45 ± 2). Also, six dairy cows were ovariectomized in proestrus and ovarian histological sections were obtained. Growth hormone mRNA was detected in granulose cells from ovarian follicle sampled during PP. A PPD × T interaction was observed for GHR mRNA, where it was greater in the FPPD cows than in the MPPD cows at T1. Metabolic biomarkers and reproductive hormones showed differences or interaction between PPD, T, S, depending on the case. Also, GH and GHR were immunolocalized in granulosa and theca interna cells of preovulatory follicles. These results confirm the expression of GH and GHR in the mature ovarian follicles of dairy cows and show a possible association between greater GHR expression and an earlier resumption of postpartum follicular development.

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来源期刊
Reproductive biology
Reproductive biology 生物-生殖生物学
CiteScore
3.90
自引率
0.00%
发文量
95
审稿时长
29 days
期刊介绍: An official journal of the Society for Biology of Reproduction and the Institute of Animal Reproduction and Food Research of Polish Academy of Sciences in Olsztyn, Poland. Reproductive Biology is an international, peer-reviewed journal covering all aspects of reproduction in vertebrates. The journal invites original research papers, short communications, review articles and commentaries dealing with reproductive physiology, endocrinology, immunology, molecular and cellular biology, receptor studies, animal breeding as well as andrology, embryology, infertility, assisted reproduction and contraception. Papers from both basic and clinical research will be considered.
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