使用 Bioprotect 和红葡萄单宁提取物对蛋白粉进行体外蛋白质保护

Bereket Zeleke Tunkala, Kristy DiGiacomo, Pablo S. Alvarez Hess, Frank R. Dunshea, Brian J. Leury
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摘要

保护瘤胃中的粗蛋白可减少反刍动物体内蛋白质的大量降解和氨的排放,并增加可利用的旁路蛋白。本实验使用 ANKOM 体外产气系统测定了两种生物保护剂(15 和 30 mL/kg 干物质 (DM))和两种单宁提取物(TE)(20 和 40 g/kg DM)的添加量对蛋白保护和培养 24 小时的菜籽粕和豆粕体外发酵特性的影响。与未处理的菜籽粕和豆粕相比,处理后的菜籽粕和豆粕产生的可溶性蛋白质("a "部分)较低,而缓慢降解蛋白质("b "部分)较高,p < 0.01。然而,与其他处理方法相比,添加 20 g/kg DM TE 对两种膳食中蛋白质组分 "a "和 "b "含量的影响最小。添加剂浓度的增加降低了总挥发性脂肪酸(VFA),p < 0.001。添加剂对不同处理的影响不同,因为 15 mL/kg DM Bioprotect 和 20 g/kg DM TE 不会影响乙酸与丙酸的比率(A:P)以及开始产气之前的时间。馏分'b'的增加和蛋白质馏分'a'的减少证实了本实验成功地保护了蛋白质。然而,在使用 30 mL/kg DM 生物保护剂后,氨氮和体外可降解蛋白质大量减少,这表明在较高剂量下可能会对负责消化蛋白质的微生物产生毒性。因此,15 毫升/千克 DM 生物保护剂和 40 克/千克 DM TE 可能是体外实验中保护蛋白质的理想剂量。
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In vitro protein protection of protein meals using Bioprotect and tannin extract from red grape marc

Protecting crude protein in the rumen may reduce extensive protein degradation and ammonia emission and increase available bypass protein in ruminants. This experiment was conducted to determine the effect of two Bioprotect (15 and 30 mL/kg dry matter (DM)) and two tannin extract (TE) (20 and 40 g/kg DM) inclusion rates on protein protection and in vitro fermentation characteristics of canola and soybean meals incubated for 24 h using an ANKOM in vitro gas production system. The treated canola and soybean meals produced lower soluble protein (fraction ‘a’) and larger slowly degradable protein (fraction ‘b’) than its untreated counterparts, p < 0.01. However, the 20 g/kg DM TE inclusion showed lowest effect on the amount of protein fractions ‘a’ and ‘b’ in both meals compared to their other treated counterparts. The increasing concentration of additives reduced the total volatile fatty acids (VFA), p < 0.001. The effects of additives differed between the treatments as 15 mL/kg DM Bioprotect and 20 g/kg DM TE did not affect the acetic to propionic acid ratio (A:P) and the time before gas production began. The increase in fraction ‘b’ and reduction in protein fraction ‘a’ confirm successful protein protection in this experiment. However, the extensive reduction in ammonia-N and in vitro degradable protein after using 30 mL/kg DM Bioprotect suggests possible toxicity to the microbes responsible for protein digestion in higher doses. Therefore, 15 mL/kg DM Bioprotect and 40 g/kg DM TE could be promising protein protection doses for in vitro experiments.

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