PBF-LB 制作微槽,用于诱导人类间充质干细胞的成骨分化

IF 6.8 3区 医学 Q1 ENGINEERING, BIOMEDICAL International Journal of Bioprinting Pub Date : 2024-01-09 DOI:10.36922/ijb.1425
Aira Matsugaki, Tadaaki Matsuzaka, Toko Mori, Mitsuka Saito, Kazuma Funaoku, Riku Yamano, O. Gokcekaya, Ryosuke Ozasa, Takayoshi Nakano
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引用次数: 0

摘要

干细胞分化对生物医学设备设计和组织工程具有重要意义。最近,人们发现材料的固有特性,包括表面化学、硬度和形貌,会影响干细胞的命运。其中,表面形貌是干细胞与材料接触的关键调节因素。理想的骨组织工程最重要的一点是控制骨细胞外基质与完全分化的成骨细胞的组织。在这里,我们发现激光粉末床融合(PBF-LB)制造的凹槽表面受骨骼微观结构的启发,可诱导人间充质干细胞(hMSC)分化为成骨系,而无需任何分化补充剂。周期性沟槽结构由 PBF-LB 制造,细胞骨架张力沿沟槽促进了细胞伸长。这通过 Runx2 的表达上调了成骨过程。排列整齐的 hMSCs 成功分化为成骨细胞,并进一步组织了以仿骨为导向的细胞外基质微结构。我们的研究结果表明,金属增材制造技术在控制干细胞向成骨系的命运以及构建仿骨微结构组织方面具有很大的优势。我们关于标准细胞培养条件下材料诱导干细胞分化的研究结果,为开发医疗设备开辟了新途径,这些设备可通过调节干细胞功能实现预期的组织再生。
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PBF-LB fabrication of microgrooves for induction of osteogenic differentiation of human mesenchymal stem cells
Stem cell differentiation has important implications for biomedical device design and tissue engineering. Recently, inherent material properties, including surface chemistry, stiffness, and topography, have been found to influence stem cell fate. Among these, surface topography is a key regulator of stem cells in contact with materials. The most important aspect of ideal bone tissue engineering is to control the organization of the bone extracellular matrix with fully differentiated osteoblasts. Here, we found that laser powder bed fusion (PBF-LB)-fabricated grooved surface inspired by the microstructure of bone, which induced human mesenchymal stem cell (hMSC) differentiation into the osteogenic lineage without any differentiation supplements. The periodic grooved structure was fabricated by PBF-LB which induced cell elongation facilitated by cytoskeletal tension along the grooves. This resulted in the upregulation of osteogenesis via Runx2 expression. The aligned hMSCs successfully differentiated into osteoblasts and further organized the bone mimetic-oriented extracellular matrix microstructure. Our results indicate that metal additive manufacturing technology has a great advantage in controlling stem cell fate into the osteogenic lineage, and in the construction of bone-mimetic microstructural organization. Our findings on material-induced stem cell differentiation under standard cell culture conditions open new avenues for the development of medical devices that realize the desired tissue regeneration mediated by regulated stem cell functions.
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来源期刊
CiteScore
6.90
自引率
4.80%
发文量
81
期刊介绍: The International Journal of Bioprinting is a globally recognized publication that focuses on the advancements, scientific discoveries, and practical implementations of Bioprinting. Bioprinting, in simple terms, involves the utilization of 3D printing technology and materials that contain living cells or biological components to fabricate tissues or other biotechnological products. Our journal encompasses interdisciplinary research that spans across technology, science, and clinical applications within the expansive realm of Bioprinting.
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