{"title":"马尼拉蝗虫(直翅目:吖啶科)的 DNA 条形码揭示了物种和亚种的分化1","authors":"Michelle S. Guerrero, B. Cayabyab","doi":"10.18474/jes23-36","DOIUrl":null,"url":null,"abstract":"\n Accurate identification and classification of insect species, especially those with significant economic and ecological implications, have historically presented challenges. Migratory locusts, Locusta migratoria manilensis (Meyen) (Orthoptera: Acrididae), are notorious for their destructive impact on crops. Traditional morphological methods often face limitations in distinguishing closely related species and require taxonomic expertise. However, the emergence of DNA barcoding as a powerful tool for species identification has revolutionized the field of entomology. DNA barcoding utilizes a standardized DNA sequence, a molecular barcode, which serves as a distinct genetic signature for rapid and accurate species identification. In this study, DNA barcoding techniques were employed to identify and differentiate the migratory locust subspecies manilensis, in both its solitary and gregarious forms, as well as to determine its phylogenetic relationship with other related species within the Acrididae family. GenBank reference sequences were used to identify the locusts at the molecular subspecies level. Although the COI marker did not exhibit significant differences between the solitary and migratory forms, it was valuable in resolving the identification of L. migratoria subspecies. This lack of significant differences may be attributed to limited genetic variation of COI at the subspecies level and substantial genetic similarities between the solitary and migratory forms, likely stemming from a recent common ancestor. Nonetheless, using COI remains beneficial for subspecies identification in migratory locusts.","PeriodicalId":15765,"journal":{"name":"Journal of Entomological Science","volume":null,"pages":null},"PeriodicalIF":0.7000,"publicationDate":"2024-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"DNA Barcoding of Locusta migratoria manilensis (Orthoptera: Acrididae) Reveals Insights into the Species and Subspecies Differentiation1\",\"authors\":\"Michelle S. Guerrero, B. Cayabyab\",\"doi\":\"10.18474/jes23-36\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"\\n Accurate identification and classification of insect species, especially those with significant economic and ecological implications, have historically presented challenges. Migratory locusts, Locusta migratoria manilensis (Meyen) (Orthoptera: Acrididae), are notorious for their destructive impact on crops. Traditional morphological methods often face limitations in distinguishing closely related species and require taxonomic expertise. However, the emergence of DNA barcoding as a powerful tool for species identification has revolutionized the field of entomology. DNA barcoding utilizes a standardized DNA sequence, a molecular barcode, which serves as a distinct genetic signature for rapid and accurate species identification. In this study, DNA barcoding techniques were employed to identify and differentiate the migratory locust subspecies manilensis, in both its solitary and gregarious forms, as well as to determine its phylogenetic relationship with other related species within the Acrididae family. GenBank reference sequences were used to identify the locusts at the molecular subspecies level. Although the COI marker did not exhibit significant differences between the solitary and migratory forms, it was valuable in resolving the identification of L. migratoria subspecies. This lack of significant differences may be attributed to limited genetic variation of COI at the subspecies level and substantial genetic similarities between the solitary and migratory forms, likely stemming from a recent common ancestor. 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引用次数: 0
摘要
昆虫物种的准确识别和分类,尤其是对经济和生态有重大影响的昆虫物种的准确识别和分类,历来是一项挑战。迁飞蝗虫 Locusta migratoria manilensis (Meyen) (直翅目:蝼蛄科)因其对农作物的破坏性影响而臭名昭著。传统的形态学方法在区分近缘物种时往往受到限制,而且需要专业的分类知识。然而,DNA 条形码作为一种强大的物种鉴定工具的出现彻底改变了昆虫学领域。DNA 条形码利用标准化的 DNA 序列(即分子条形码)作为独特的遗传特征,用于快速准确地鉴定物种。本研究利用 DNA 条形码技术鉴定和区分迁徙蝗虫亚种 manilensis 的单生和群生形态,并确定其与刺蝗科其他相关物种的系统发育关系。研究人员利用 GenBank 参考序列对蝗虫进行分子亚种鉴定。虽然COI标记在独居型和迁徙型蝗虫之间没有表现出显著差异,但它在确定迁徙型蝗虫亚种方面很有价值。这种差异不明显的原因可能是 COI 在亚种水平上的遗传变异有限,而独居型和迁徙型之间的遗传相似性很大,很可能源于最近的共同祖先。尽管如此,使用 COI 仍然有利于迁徙蝗虫的亚种鉴定。
DNA Barcoding of Locusta migratoria manilensis (Orthoptera: Acrididae) Reveals Insights into the Species and Subspecies Differentiation1
Accurate identification and classification of insect species, especially those with significant economic and ecological implications, have historically presented challenges. Migratory locusts, Locusta migratoria manilensis (Meyen) (Orthoptera: Acrididae), are notorious for their destructive impact on crops. Traditional morphological methods often face limitations in distinguishing closely related species and require taxonomic expertise. However, the emergence of DNA barcoding as a powerful tool for species identification has revolutionized the field of entomology. DNA barcoding utilizes a standardized DNA sequence, a molecular barcode, which serves as a distinct genetic signature for rapid and accurate species identification. In this study, DNA barcoding techniques were employed to identify and differentiate the migratory locust subspecies manilensis, in both its solitary and gregarious forms, as well as to determine its phylogenetic relationship with other related species within the Acrididae family. GenBank reference sequences were used to identify the locusts at the molecular subspecies level. Although the COI marker did not exhibit significant differences between the solitary and migratory forms, it was valuable in resolving the identification of L. migratoria subspecies. This lack of significant differences may be attributed to limited genetic variation of COI at the subspecies level and substantial genetic similarities between the solitary and migratory forms, likely stemming from a recent common ancestor. Nonetheless, using COI remains beneficial for subspecies identification in migratory locusts.
期刊介绍:
The Journal of Entomological Science (ISSN 0749-8004) is a peer-reviewed, scholarly journal that is published quarterly (January, April, July, and October) under the auspices of the Georgia Entomological Society in concert with Allen Press (Lawrence, Kansas). Manuscripts deemed acceptable for publication in the Journal report original research with insects and related arthropods or literature reviews offering foundations to innovative directions in entomological research