{"title":"优化影响农杆菌介导的狼尾草转化效率的因素","authors":"A. N. Shvedova, P. A. Khvatkov, S. V. Dolgov","doi":"10.1134/S0003683823090089","DOIUrl":null,"url":null,"abstract":"<div><p>Plants of the duckweed family (<i>Lemna</i>, <i>Spirodela</i>) are actively used as expression platforms for the production of recombinant proteins due to a number of their advantages over other crops (small size, rapid vegetative reproduction, and high protein content in tissues). Rootless wolffia (<i>Wolffia arrhiza</i> (L.) Horkel ex Wimm) is a promising producer from the Lemnaceae family. Current <i>Wolffia</i> transformation protocols have an efficiency of approximately 0.36%. Based on transient expression, we have identified the most efficient <i>Agrobacterium</i> strain, EHA105, for the <i>W. arrhiza</i> transformation. An effective balance of growth regulators was selected for use at the first passage of cultivation and selection of transformed explants: 2,4-dichlorophenoxyacetic acid, 2.5 mg/L and 6-benzylaminopurine, 1.5 mg/L. The transformation efficiency of <i>W. arrhiza</i> was 0.54%, which is 1.5 times higher than in known protocols.</p></div>","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"59 9","pages":"1177 - 1182"},"PeriodicalIF":1.0000,"publicationDate":"2024-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Optimization of Factors Affecting the Efficiency of Agrobacterium-Mediated Transformation of Wolffia arrhiza\",\"authors\":\"A. N. Shvedova, P. A. Khvatkov, S. V. Dolgov\",\"doi\":\"10.1134/S0003683823090089\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Plants of the duckweed family (<i>Lemna</i>, <i>Spirodela</i>) are actively used as expression platforms for the production of recombinant proteins due to a number of their advantages over other crops (small size, rapid vegetative reproduction, and high protein content in tissues). Rootless wolffia (<i>Wolffia arrhiza</i> (L.) Horkel ex Wimm) is a promising producer from the Lemnaceae family. Current <i>Wolffia</i> transformation protocols have an efficiency of approximately 0.36%. Based on transient expression, we have identified the most efficient <i>Agrobacterium</i> strain, EHA105, for the <i>W. arrhiza</i> transformation. An effective balance of growth regulators was selected for use at the first passage of cultivation and selection of transformed explants: 2,4-dichlorophenoxyacetic acid, 2.5 mg/L and 6-benzylaminopurine, 1.5 mg/L. The transformation efficiency of <i>W. arrhiza</i> was 0.54%, which is 1.5 times higher than in known protocols.</p></div>\",\"PeriodicalId\":466,\"journal\":{\"name\":\"Applied Biochemistry and Microbiology\",\"volume\":\"59 9\",\"pages\":\"1177 - 1182\"},\"PeriodicalIF\":1.0000,\"publicationDate\":\"2024-01-12\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Applied Biochemistry and Microbiology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://link.springer.com/article/10.1134/S0003683823090089\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Applied Biochemistry and Microbiology","FirstCategoryId":"99","ListUrlMain":"https://link.springer.com/article/10.1134/S0003683823090089","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0
摘要
浮萍科植物（Lemna、Spirodela）与其他作物相比具有许多优势（体积小、无性繁殖快、组织中蛋白质含量高），因此被积极用作生产重组蛋白质的表达平台。无根狼尾草（Wolffia arrhiza (L.) Horkel ex Wimm）是一种很有前景的莱姆科植物。目前的狼尾草转化效率约为 0.36%。根据瞬时表达，我们确定了用于 W. arrhiza 转化的最高效农杆菌菌株 EHA105。在培养和选择转化外植体的第一阶段，我们选择了一种有效的平衡生长调节剂：2,4-二氯苯氧乙酸（2.5 毫克/升）和 6-苄基氨基嘌呤（1.5 毫克/升）。W. arrhiza 的转化效率为 0.54%，是已知方案的 1.5 倍。
Optimization of Factors Affecting the Efficiency of Agrobacterium-Mediated Transformation of Wolffia arrhiza
Plants of the duckweed family (Lemna, Spirodela) are actively used as expression platforms for the production of recombinant proteins due to a number of their advantages over other crops (small size, rapid vegetative reproduction, and high protein content in tissues). Rootless wolffia (Wolffia arrhiza (L.) Horkel ex Wimm) is a promising producer from the Lemnaceae family. Current Wolffia transformation protocols have an efficiency of approximately 0.36%. Based on transient expression, we have identified the most efficient Agrobacterium strain, EHA105, for the W. arrhiza transformation. An effective balance of growth regulators was selected for use at the first passage of cultivation and selection of transformed explants: 2,4-dichlorophenoxyacetic acid, 2.5 mg/L and 6-benzylaminopurine, 1.5 mg/L. The transformation efficiency of W. arrhiza was 0.54%, which is 1.5 times higher than in known protocols.
期刊介绍：
Applied Biochemistry and Microbiology is an international peer reviewed journal that publishes original articles on biochemistry and microbiology that have or may have practical applications. The studies include: enzymes and mechanisms of enzymatic reactions, biosynthesis of low and high molecular physiologically active compounds; the studies of their structure and properties; biogenesis and pathways of their regulation; metabolism of producers of biologically active compounds, biocatalysis in organic synthesis, applied genetics of microorganisms, applied enzymology; protein and metabolic engineering, biochemical bases of phytoimmunity, applied aspects of biochemical and immunochemical analysis; biodegradation of xenobiotics; biosensors; biomedical research (without clinical studies). Along with experimental works, the journal publishes descriptions of novel research techniques and reviews on selected topics.
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