Janthinobacterium sp. 菌株 E1 在压力条件下对邻苯二甲酸酯 (PAE) 的生物降解。

IF 0.8 4区 生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Journal of General and Applied Microbiology Pub Date : 2024-07-20 Epub Date: 2024-01-15 DOI:10.2323/jgam.2023.12.002
Kailu Zhang, Hui Zhou, Juntao Ke, Hongli Feng, Cunlong Lu, Shaoxing Chen, Aimin Liu
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引用次数: 0

摘要

邻苯二甲酸酯(PAEs)是一种高分子材料添加剂,被广泛添加到塑料中以提高产品的柔韧性。它容易造成环境污染,危害公众健康。本研究分离了一种高效降解 PAEs 的菌株 Janthinobacterium sp. E1,并测定了其在胁迫条件下对邻苯二甲酸二-2-乙基己酯(DEHP)的降解效果。在各种环境胁迫条件下,菌株E1在污染物降解方面表现出明显的优势。在添加了甘油三酯的琼脂平板上,菌株 E1 的菌落周围出现了明显的降解光环。菌株 E1 的酯酶是一种组成型细胞内表达酶。从菌株 E1 中纯化的酯酶对短链 PAE 的催化作用高于长链 PAE。DEHP、DBP(邻苯二甲酸二丁酯)和 DMP(邻苯二甲酸二甲酯)进入测试土壤后,并未改变土壤原核生物群落的物种组成,但改变了特定环境条件下的优势物种。群落的多样性和丰富度在一定程度上有所下降。然而,用 E1 菌株处理污染土壤后,微生物群落的多样性和丰富度都有所提高。我们的研究结果还表明,菌株 E1 在现实环境中的环境生物修复中表现出了巨大的潜力,这为消除城市环境中的污染物污染提供了新的思路。
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Biodegradation of phthalic acid esters (PAEs) by Janthinobacterium sp. strain E1 under stress conditions.

Phthalates esters (PAEs) are a kind of polymeric material additives widely been added into plastics to improve products' flexibility. It can easily cause environmental pollution which are hazards to public health. In this study, we isolated an efficient PAEs degrading strain, Janthinobacterium sp. E1, and determined its degradation effect of di-2-ethylhexyl phthalate (DEHP) under stress conditions. Strain E1 showed an obvious advantage in pollutants degradation under various environmental stress conditions. Degradation halo clearly occurred around the colony of strain E1 on agar plate supplemented with triglyceride. Strain E1's esterase is a constitutively expressed intracellular enzyme. The esterase purified from strain E1 showed a higher catalytic effect on short-chain PAEs than long-chain PAEs. The input of DEHP, DBP (dibutyl phthalate) and DMP (dimethyl phthalate) into the tested soil did not change the species composition of soil prokaryotic community, but altered the dominant species in specific environmental conditions. And the community diversity and richness decreased to a certain extent. However, the diversity and richness of the microbial community were improved after the contaminated soil was treated with the strain E1. Our results also suggested that strain E1 exhibited a tremendous potential in environmental bioremediation in the real environment, which provides a new insight into the elimination of the pollutants contamination in the urban environment.

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来源期刊
Journal of General and Applied Microbiology
Journal of General and Applied Microbiology 生物-生物工程与应用微生物
CiteScore
2.40
自引率
0.00%
发文量
42
审稿时长
6-12 weeks
期刊介绍: JGAM is going to publish scientific reports containing novel and significant microbiological findings, which are mainly devoted to the following categories: Antibiotics and Secondary Metabolites; Biotechnology and Metabolic Engineering; Developmental Microbiology; Environmental Microbiology and Bioremediation; Enzymology; Eukaryotic Microbiology; Evolution and Phylogenetics; Genome Integrity and Plasticity; Microalgae and Photosynthesis; Microbiology for Food; Molecular Genetics; Physiology and Cell Surface; Synthetic and Systems Microbiology.
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