明确阐明锐尖叶蝉接种Xylella fastidiosa的机制

IF 5.6 1区 农林科学 Q1 ENTOMOLOGY Entomologia Generalis Pub Date : 2024-01-16 DOI:10.1127/entomologia/2023/2126
Daniele Cornara, Isabel Bojanini, Alberto Fereres, Rodrig.P.P. Almeida
{"title":"明确阐明锐尖叶蝉接种Xylella fastidiosa的机制","authors":"Daniele Cornara, Isabel Bojanini, Alberto Fereres, Rodrig.P.P. Almeida","doi":"10.1127/entomologia/2023/2126","DOIUrl":null,"url":null,"abstract":"Xylella fastidiosa is a vector-borne xylem-limited bacterium of agricultural and economic importance, yet it remains to be demonstrated how this pathogen is inoculated into plants by insect vectors. Recent DC-EPG (Electrical Penetration Graph) research with the meadow spittlebug, Philaenus spumarius tentatively correlated bacterial inoculation with a non-stereotypical “spiking waveform” termed Xe; this behavior occurred within the first minutes from initial stylets contact with a xylem vessel. Here, using the Graphocephala atropunctata-grapevine-X. fastidiosa pathosystem, we conclusively demonstrate that the DC-EPG waveform Xe represents the vector probing behavior correlated with X. fastidiosa inoculation into a host plant, and successful systemic persistent pathogen infection. Our work supports the previous hypothesis that Xe represents an egestion behavior, with a mixture of xylem sap-bacterial cells propelled by the collapse of the cibarial diaphragm while the precibarial valve is fluttering, potentially to remove obstructions from the precibarium and its chemosensilla. The outward flow delivering the mixture sap-bacterial cells into the plant is permitted by the valve that flutters instead of sealing the food canal. The inoculation behavior Xe is more frequent in vectors harboring X. fastidiosa cells within the foregut, independent of the bacterial population size in insects.","PeriodicalId":11728,"journal":{"name":"Entomologia Generalis","volume":null,"pages":null},"PeriodicalIF":5.6000,"publicationDate":"2024-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Definitive elucidation of the inoculation mechanism of Xylella fastidiosa by sharpshooter leafhoppers\",\"authors\":\"Daniele Cornara, Isabel Bojanini, Alberto Fereres, Rodrig.P.P. Almeida\",\"doi\":\"10.1127/entomologia/2023/2126\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Xylella fastidiosa is a vector-borne xylem-limited bacterium of agricultural and economic importance, yet it remains to be demonstrated how this pathogen is inoculated into plants by insect vectors. Recent DC-EPG (Electrical Penetration Graph) research with the meadow spittlebug, Philaenus spumarius tentatively correlated bacterial inoculation with a non-stereotypical “spiking waveform” termed Xe; this behavior occurred within the first minutes from initial stylets contact with a xylem vessel. Here, using the Graphocephala atropunctata-grapevine-X. fastidiosa pathosystem, we conclusively demonstrate that the DC-EPG waveform Xe represents the vector probing behavior correlated with X. fastidiosa inoculation into a host plant, and successful systemic persistent pathogen infection. Our work supports the previous hypothesis that Xe represents an egestion behavior, with a mixture of xylem sap-bacterial cells propelled by the collapse of the cibarial diaphragm while the precibarial valve is fluttering, potentially to remove obstructions from the precibarium and its chemosensilla. The outward flow delivering the mixture sap-bacterial cells into the plant is permitted by the valve that flutters instead of sealing the food canal. The inoculation behavior Xe is more frequent in vectors harboring X. fastidiosa cells within the foregut, independent of the bacterial population size in insects.\",\"PeriodicalId\":11728,\"journal\":{\"name\":\"Entomologia Generalis\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":5.6000,\"publicationDate\":\"2024-01-16\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Entomologia Generalis\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1127/entomologia/2023/2126\",\"RegionNum\":1,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"ENTOMOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Entomologia Generalis","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1127/entomologia/2023/2126","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENTOMOLOGY","Score":null,"Total":0}
引用次数: 0

摘要

Xylella fastidiosa 是一种病媒传播的木质部局限性细菌,对农业和经济具有重要意义,但这种病原体是如何通过昆虫载体接种到植物中的仍有待证实。最近对草地唾液蝽(Phaenus spumarius)进行的 DC-EPG(电穿透图)研究初步证实,细菌接种与一种称为 Xe 的非定型 "尖峰波形 "有关;这种行为发生在花柱最初接触木质部血管的最初几分钟内。在这里,我们利用 Graphocephala atropunctata-grapevine-X. fastidiosa 病理系统,确证了直流电谱波形 Xe 代表了与 X. fastidiosa 接种到寄主植物并成功造成系统性持续病原体感染相关的载体探测行为。我们的工作支持了之前的假设,即 Xe 代表了木质部汁液-细菌细胞混合物的排出行为,当木质部前瓣膜扇动时,木质部汁液-细菌细胞混合物被塌陷的木质部前瓣膜推动,可能是为了清除木质部前瓣膜及其化学感受器的阻塞。由于瓣膜的扇动而不是封闭食道,因此可以将汁液和细菌细胞的混合物向外输送到植物体内。在前肠中携带 X. fastidiosa 细胞的媒介中,Xe 的接种行为更为频繁,这与昆虫体内的细菌数量无关。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
Definitive elucidation of the inoculation mechanism of Xylella fastidiosa by sharpshooter leafhoppers
Xylella fastidiosa is a vector-borne xylem-limited bacterium of agricultural and economic importance, yet it remains to be demonstrated how this pathogen is inoculated into plants by insect vectors. Recent DC-EPG (Electrical Penetration Graph) research with the meadow spittlebug, Philaenus spumarius tentatively correlated bacterial inoculation with a non-stereotypical “spiking waveform” termed Xe; this behavior occurred within the first minutes from initial stylets contact with a xylem vessel. Here, using the Graphocephala atropunctata-grapevine-X. fastidiosa pathosystem, we conclusively demonstrate that the DC-EPG waveform Xe represents the vector probing behavior correlated with X. fastidiosa inoculation into a host plant, and successful systemic persistent pathogen infection. Our work supports the previous hypothesis that Xe represents an egestion behavior, with a mixture of xylem sap-bacterial cells propelled by the collapse of the cibarial diaphragm while the precibarial valve is fluttering, potentially to remove obstructions from the precibarium and its chemosensilla. The outward flow delivering the mixture sap-bacterial cells into the plant is permitted by the valve that flutters instead of sealing the food canal. The inoculation behavior Xe is more frequent in vectors harboring X. fastidiosa cells within the foregut, independent of the bacterial population size in insects.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Entomologia Generalis
Entomologia Generalis 生物-昆虫学
CiteScore
7.10
自引率
18.80%
发文量
72
审稿时长
>12 weeks
期刊介绍: Its scope covers all aspects of basic and applied research dealing with insects and more broadly with arthropods inhabiting wild, agricultural and/or urban habitats. The journal also considers research integrating various disciplines and issues within the broad field of entomology and ecology. Entomologia Generalis publishes high quality research articles on advances in knowledge on the ecology and biology of arthropods, as well as on their importance for key ecosystems services, e.g. as biological control and pollination. The journal devotes special attention to contributions providing significant advances (i) on the fundamental knowledge and on sustainable control strategies of arthropod pests (including of stored products) and vectors of diseases, (ii) on the biology and ecology of beneficial arthropods, (iii) on the spread and impact of invasive pests, and (iv) on potential side effects of pest management methods. Entomologia Generalis welcomes review articles on significant developments in the field of entomology. These are usually invited by the editorial board, but proposals may be sent to the Editor-in-Chief for preliminary assessment by the editorial board before formal submission to the journal. The journal also considers comments on papers published in Entomologia Generalis, as well as short notes on topics that are of broader interest.
期刊最新文献
Sublethal and transgenerational effects of chlorfenapyr on physiological and developmental traits of Spodoptera frugiperda Unraveling the feeding response and intergenerational sublethal effects of flonicamid on Rhopalosiphum padi A novel potential nAChR variation and the upregulation of CYP304A1 contribute to resistance against lambda-cyhalothrin and spinosad in Bactrocera dorsalis Ultraviolet and violet light enhance the attraction efficacy of semiochemicals for the Asian longhorned beetle Compensating from a bad start in life: linking male energy reserves to reproductive output in a polyphagous moth
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1