LncRNA HCG11通过miR-17-5p/p21轴增强非小细胞肺癌细胞对吉西他滨的化疗敏感性

IF 2.9 3区医学 Q2 ONCOLOGY Expert Review of Anticancer Therapy Pub Date : 2024-01-01 Epub Date: 2024-02-12 DOI:10.1080/14737140.2024.2305352

Yufen Xu, Xiaoli Tan, Qi Yang, Zhixian Fang, Wenyu Chen

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引用次数: 0

摘要

研究背景本研究探讨了lncRNA HLA复合体第11组（HCG11）对非小细胞肺癌（NSCLC）的抑制作用及其分子机制：采用生物信息学分析确定下游靶基因miR-17-5p/p21并预测其结合位点，采用qRT-PCR和Western blot检测表达水平，采用双荧光素酶和RIP实验验证结合关系：结果发现：lncRNA HCG11/miR-17-5p/p21轴调控A549和A549-吉西他滨（GEM）细胞的耐药性、增殖、凋亡和细胞周期。HCG11 作为一种 ceRNA 与 miR-17-5p 结合，进而抑制 p21 的表达。体内实验表明，HCG11阻碍了肿瘤的生长。因此，lncRNA HCG11通过靶向miR-17-5p/p21轴，抑制了NSCLC细胞的GEM耐药性和恶性进展：本研究为研究lncRNA HCG11在NSCLC诊断中的潜在价值以及寻找NSCLC临床化疗耐药的潜在靶点提供了参考。

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LncRNA HCG11 enhances the chemosensitivity of non-small cell lung cancer cells to Gemcitabine via miR-17-5p/p21 axis.

Background: This study investigated the inhibitory effects of lncRNA HLA Complex Group 11 (HCG11) on non-small cell lung cancer (NSCLC) and the molecular mechanisms.

Research design and methods: Bioinformatics analysis was conducted to determine the downstream targeted gene miR-17-5p/p21 and predict their binding sites. qRT-PCR and Western blot were used to detect expression levels, and dual luciferase and RIP assays were adopted to verify binding relationship.

Results: The lncRNA HCG11/miR-17-5p/p21 axis was found to regulate drug resistance, proliferation, apoptosis, and cell cycle of A549 and A549-Gemcitabine (GEM) cells. HCG11 acted as a ceRNA binding to miR-17-5p, which repressed p21 expression in turn. In vivo experiments demonstrated that HCG11 hindered tumor growth. Therefore, lncRNA HCG11, by targeting the miR-17-5p/p21 axis, suppressed GEM resistance and malignant progression of NSCLC cells.

Conclusions: This study provides a reference for investigating the potential value of lncRNA HCG11 in the diagnosis of NSCLC and finding potential targets against clinical chemotherapeutic resistance in NSCLC.

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来源期刊

Expert Review of Anticancer Therapy 医学-肿瘤学

CiteScore

5.10

自引率

3.00%

发文量

100

审稿时长

4-8 weeks

期刊介绍： Expert Review of Anticancer Therapy (ISSN 1473-7140) provides expert appraisal and commentary on the major trends in cancer care and highlights the performance of new therapeutic and diagnostic approaches. Coverage includes tumor management, novel medicines, anticancer agents and chemotherapy, biological therapy, cancer vaccines, therapeutic indications, biomarkers and diagnostics, and treatment guidelines. All articles are subject to rigorous peer-review, and the journal makes an essential contribution to decision-making in cancer care. Comprehensive coverage in each review is complemented by the unique Expert Review format and includes the following sections: Expert Opinion - a personal view of the data presented in the article, a discussion on the developments that are likely to be important in the future, and the avenues of research likely to become exciting as further studies yield more detailed results Article Highlights – an executive summary of the author’s most critical points.