利用人体脂肪间充质干细胞(hADMSCs)和 PCL/层粘连蛋白支架基底合成骨生物相容性植入物。

IF 2.1 4区 医学 Q3 MEDICINE, RESEARCH & EXPERIMENTAL Iranian Journal of Basic Medical Sciences Pub Date : 2024-01-01 DOI:10.22038/IJBMS.2023.71307.15491
Donya Zeydari, Ehsan Karimi, Ehsan Saburi
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引用次数: 0

摘要

目的:骨组织工程被认为是治疗骨缺损的一种新方法,可以有效替代手术和植骨。在合成聚合物支架上使用脂肪组织间充质干细胞(ADMSCs)是骨组织工程的新方法之一。本研究旨在探讨层粘连蛋白涂层对聚己内酯(PCL)支架上播种的 ADMSCs 的生物相容性和成骨分化的影响:使用扫描电子显微镜(SEM)评估电纺支架的形态。细胞增殖和细胞毒性采用 MTT 法测定。评估了细胞的成脂和成骨分化潜能。通过评估碱性磷酸酶(ALP)活性、细胞内钙含量和骨特异性基因的表达水平,评估了在覆有层粘蛋白的 PCL 支架上培养的 ADMSCs 的成骨分化情况:结果表明,与在无涂层 PCL 或对照培养基(PC)上培养的 ADMSCs 相比,在 PCL/层粘连蛋白上培养的 ADMSCs 显示出更强的成骨分化能力:看来,层粘连蛋白增强了 PCL 纳米纤维支架的理化性质和生物相容性,并通过修饰支架表面改善了 ADMSCs 向成骨细胞的分化。
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Synthesis of bone biocompatible implants using human adipose-derived mesenchymal stem cells (hADMSCs) and PCL/laminin scaffold substrate.

Objectives: Bone tissue engineering is considered a new method in the treatment of bone defects and can be an effective alternative to surgery and bone grafting. The use of adipose tissue mesenchymal stem cells (ADMSCs) on synthetic polymer scaffolds is one of the new approaches in bone tissue engineering. In this study, we aimed to investigate the effect of laminin coating on biocompatibility and osteogenic differentiation of ADMSCs seeded on polycaprolactone (PCL) scaffolds.

Materials and methods: The morphology of the electrospun scaffold was evaluated using a scanning electron microscope (SEM). Cell proliferation and cytotoxicity were determined by MTT assay. The adipogenic and osteogenic differentiation potential of the cells was evaluated. The osteogenic differentiation of ADMSCs cultured on the PCL scaffold coated with laminin was assessed by evaluating the level of alkaline phosphatase (ALP) activity, intracellular calcium content, and expression of bone-specific genes.

Results: The results showed that the ADMSCs cultured on PCL/laminin showed enhanced osteogenic differentiation compared to those cultured on non-coated PCL or control medium (P<0.05).

Conclusion: It seems that laminin enhances the physicochemical properties and biocompatibility of PCL nanofiber scaffolds; and by modifying the surface of the scaffold, improves the differentiation of ADMSCs into osteogenic cells.

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来源期刊
Iranian Journal of Basic Medical Sciences
Iranian Journal of Basic Medical Sciences MEDICINE, RESEARCH & EXPERIMENTAL-PHARMACOLOGY & PHARMACY
CiteScore
4.00
自引率
4.50%
发文量
142
审稿时长
6-12 weeks
期刊介绍: The Iranian Journal of Basic Medical Sciences (IJBMS) is a peer-reviewed, monthly publication by Mashhad University of Medical Sciences (MUMS), Mashhad, Iran . The Journal of "IJBMS” is a modern forum for scientific communication. Data and information, useful to investigators in any discipline in basic medical sciences mainly including Anatomical Sciences, Biochemistry, Genetics, Immunology, Microbiology, Pathology, Pharmacology, Pharmaceutical Sciences, and Physiology, will be published after they have been peer reviewed. This will also include reviews and multidisciplinary research.
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