Victória Veiga Alves, Letícia Cury Rocha Veloso Arantes, Dayse Helena Lages da Silva, Eric Santos Oliveira, Julia Figueiredo de Souza, Mailson Teixeira da Silva, Matheus Dias Araújo, Rodrigo Dias de Oliveira Carvalho, João Luís Reis Cunha, Leonardo José Camargos Lara, Roselene Ecco, Nelson Rodrigo da Silva Martins, Paul Andrew Barrow, Oliveiro Caetano de Freitas Neto
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引用次数: 0
摘要
摘要 海德堡沙门氏菌（Salmonella Heidelberg，SH）致病性和抗菌性分离株的日益流行是一个全球关注的问题。因此，需要采取新的策略来预防和控制由这种细菌引起的感染。本研究旨在探讨单独或与含有胞嘧啶-磷酸二酯-鸟嘌呤（CpG-ODN）的寡脱氧核苷酸联合使用以细菌肽为基础的配方进行体内注射对雏鸡感染海德堡沙门氏菌的保护作用。通过分析公共数据库中的 SH 基因组数据，筛选出结构蛋白的氨基酸序列或与肠道定植更相关的氨基酸序列。对这些序列进行线性表位预测，以确定高免疫原性肽。对 180 枚 SPF 卵进行孵化，并在孵化第 18 天通过尿囊腔注射。结果表明，在感染后的第一周，卵内注射肽+CpG-ODN可减少SH在盲肠内容物中的定植，但在整个研究过程中并没有减少粪便的总体排泄量。此外，CpG-ODN注射可能会对肠道健康产生积极影响，在21 dpi时隐窝深度降低，28 dpi时绒毛高度增加就是证明。各组雏鸡的分泌型 IgA 水平没有差异，在整个研究过程中，任何感染组的雏鸡肝脏中都没有发现可检测到的 SH 计数。总之，肽+CpG-ODN的体内注射可能有助于在感染早期减少SH在盲肠中的定植，但不会影响粪便的总排泄量。此外，CpG-ODN注射液可能会改善雏鸡的肠道健康参数。
Effects of in ovo injection of bacterial peptides and CpG-ODN on Salmonella enterica serovar Heidelberg infection in specific pathogen-free (SPF) chicks.
Research highlights: Peptides + CpG-ODN reduced SH in caeca at the first week post-infection.Administered formulations did not reduce SH-faecal excretion.Levels of intestinal IgA were similar between all groups.CpG-ODN improved some parameters associated with chick intestinal health.
期刊介绍：
Avian Pathology is the official journal of the World Veterinary Poultry Association and, since its first publication in 1972, has been a leading international journal for poultry disease scientists. It publishes material relevant to the entire field of infectious and non-infectious diseases of poultry and other birds. Accepted manuscripts will contribute novel data of interest to an international readership and will add significantly to knowledge and understanding of diseases, old or new. Subject areas include pathology, diagnosis, detection and characterisation of pathogens, infections of possible zoonotic importance, epidemiology, innate and immune responses, vaccines, gene sequences, genetics in relation to disease and physiological and biochemical changes in response to disease. First and subsequent reports of well-recognized diseases within a country are not acceptable unless they also include substantial new information about the disease or pathogen. Manuscripts on wild or pet birds should describe disease or pathogens in a significant number of birds, recognizing/suggesting serious potential impact on that species or that the disease or pathogen is of demonstrable relevance to poultry. Manuscripts on food-borne microorganisms acquired during or after processing, and those that catalogue the occurrence or properties of microorganisms, are unlikely to be considered for publication in the absence of data linking them to avian disease.
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