MDA-MB-231 细胞在二维和三维共培养模型中的受限迁移评估研究。

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS ACS Applied Bio Materials Pub Date : 2023-12-01 Epub Date: 2024-01-19 DOI:10.1177/15353702231214269
Zhichang Du, Shaohui Yang, Qingzhong Gong, Zhonghua Lin, Guohong Xiao, Shengli Mi
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引用次数: 0

摘要

限制性迁移评价与体内肿瘤相一致,有利于开展更复杂的肿瘤迁移研究。然而,受限细胞迁移与非受限细胞迁移之间的差异以及不同评价方法之间的区别尚未得到系统研究,阻碍了相关研究的开展。本研究通过在芯片上构建受限环境,研究了共培养条件对癌细胞迁移能力的影响。结果表明,受限通道可以通过限制肿瘤细胞的自由生长本能,分辨弱肿瘤环境因素对复杂肿瘤迁移行为的影响。通过比较二维和三维限制迁移方法,细胞外基质(ECM)限制也有助于区分弱肿瘤环境因素的影响。但三维 ECM 能更好地反映细胞迁移过程的迂回性和癌细胞之间的合作行为。在抗癌药物评估中,三维 ECM 能更准确地反映药物的细胞毒性,也更符合人体的耐药性。总之,该研究有助于区分癌细胞迁移的不同评价方法,帮助研究人员选择合适的评价模型,促进肿瘤转移的研究。
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Research of restricted migration evaluation of MDA-MB-231 cells in 2D and 3D co-culture models.

The restricted migration evaluation is conducive to more complex tumor migration research because of the conformity with in vivo tumors. However, the differences between restricted and unrestricted cell migration and the distinction between different evaluation methods have not been systematically studied, hindering related research. In this study, by constructing the restricted environments on chips, the influence of co-culture conditions on the cancer cell migration capacity was studied. The results showed that the restricted channels can discriminate the influence of weak tumor environmental factors on complex tumor migration behaviors by limiting the free growth instinct of tumor cells. Through the comparison of 2D and 3D restricted migration methods, the extracellular matrix (ECM) restriction was also helpful in distinguishing the influence of the weak tumor environmental factor. However, the 3D ECM can better reflect the tortuosity of the cell migration process and the cooperative behavior among cancer cells. In the anticancer drug evaluation, 3D ECM can more accurately reflect the cytotoxicity of drugs and is more consistent with the drug resistance in the human body. In conclusion, the research will help to distinguish different evaluation methods of cancer cell migration, help researchers select appropriate evaluation models, and promote the research of tumor metastasis.

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来源期刊
ACS Applied Bio Materials
ACS Applied Bio Materials Chemistry-Chemistry (all)
CiteScore
9.40
自引率
2.10%
发文量
464
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