两种高通量粪便顶空采样平台的优化与比较:微室/热萃取器和高容量吸附萃取探针(HiSorb)。

IF 3.7 4区 医学 Q1 BIOCHEMICAL RESEARCH METHODS Journal of breath research Pub Date : 2024-02-05 DOI:10.1088/1752-7163/ad2002
Robert van Vorstenbosch, Alex Mommers, Daniëlle Pachen, Frederik-Jan van Schooten, Agnieszka Smolinska
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引用次数: 0

摘要

使用挥发性有机化合物(VOCs)进行疾病检测和监测正变得越来越流行。对于各种(胃肠道)疾病,都应考虑微生物组。由于粪便在很大程度上具有挥发性,因此粪便挥发物组学具有很大的潜力。一个技术限制是,目前的粪便顶空分析需要专门的仪器,这种仪器成本高昂,而且通常无法与呼气研究等常用的热脱附装置协调工作。这种不协调阻碍了挥发性有机物组学界对此类分析的接受。因此,本研究对最近统一的两种粪便顶空采样平台进行了优化和比较:高容量吸附萃取(HiSorb)探头和微室热萃取器(Microchamber)。实验统计设计(DOE)通过最大限度地提高重现性、检测到的挥发性有机化合物数量以及受试者之间的差异来找到最佳采样条件。为了提高这些因素的普遍适用性,使用了半靶标和非靶标代谢曲线来定义这些因素。结果发现,HiSorb 探针的采样过程更快,检测到的挥发性有机化合物数量更多,稳定性更高。使用 Microchamber 进行顶空采集,尽管干扰挥发性有机化合物数量较少且无背景信号,但检测到的挥发性有机化合物数量较少,采样时间较长,稳定性较差。根据观察到的情况,我们对使用这两种平台中的任何一种时的预处理和研究设计提出了建议。两种平台都可用于粪便顶空采集,但建议使用 HiSorb 平台。
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The optimization and comparison of two high-throughput faecal headspace sampling platforms: the microchamber/thermal extractor and hi-capacity sorptive extraction probes (HiSorb).

Disease detection and monitoring using volatile organic compounds (VOCs) is becoming increasingly popular. For a variety of (gastrointestinal) diseases the microbiome should be considered. As its output is to large extent volatile, faecal volatilomics carries great potential. One technical limitation is that current faecal headspace analysis requires specialized instrumentation which is costly and typically does not work in harmony with thermal desorption units often utilized in e.g. exhaled breath studies. This lack of harmonization hinders uptake of such analyses by the Volatilomics community. Therefore, this study optimized and compared two recently harmonized faecal headspace sampling platforms:High-capacity Sorptive extraction (HiSorb) probesand theMicrochamber thermal extractor (Microchamber). Statistical design of experiment was applied to find optimal sampling conditions by maximizing reproducibility, the number of VOCs detected, and between subject variation. To foster general applicability those factors were defined using semi-targeted as well as untargeted metabolic profiles. HiSorb probes were found to result in a faster sampling procedure, higher number of detected VOCs, and higher stability. The headspace collection using the Microchamber resulted in a lower number of detected VOCs, longer sampling times and decreased stability despite a smaller number of interfering VOCs and no background signals. Based on the observed profiles, recommendations are provided on pre-processing and study design when using either one of both platforms. Both can be used to perform faecal headspace collection, but altogether HiSorb is recommended.

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来源期刊
Journal of breath research
Journal of breath research BIOCHEMICAL RESEARCH METHODS-RESPIRATORY SYSTEM
CiteScore
7.60
自引率
21.10%
发文量
49
审稿时长
>12 weeks
期刊介绍: Journal of Breath Research is dedicated to all aspects of scientific breath research. The traditional focus is on analysis of volatile compounds and aerosols in exhaled breath for the investigation of exogenous exposures, metabolism, toxicology, health status and the diagnosis of disease and breath odours. The journal also welcomes other breath-related topics. Typical areas of interest include: Big laboratory instrumentation: describing new state-of-the-art analytical instrumentation capable of performing high-resolution discovery and targeted breath research; exploiting complex technologies drawn from other areas of biochemistry and genetics for breath research. Engineering solutions: developing new breath sampling technologies for condensate and aerosols, for chemical and optical sensors, for extraction and sample preparation methods, for automation and standardization, and for multiplex analyses to preserve the breath matrix and facilitating analytical throughput. Measure exhaled constituents (e.g. CO2, acetone, isoprene) as markers of human presence or mitigate such contaminants in enclosed environments. Human and animal in vivo studies: decoding the ''breath exposome'', implementing exposure and intervention studies, performing cross-sectional and case-control research, assaying immune and inflammatory response, and testing mammalian host response to infections and exogenous exposures to develop information directly applicable to systems biology. Studying inhalation toxicology; inhaled breath as a source of internal dose; resultant blood, breath and urinary biomarkers linked to inhalation pathway. Cellular and molecular level in vitro studies. Clinical, pharmacological and forensic applications. Mathematical, statistical and graphical data interpretation.
期刊最新文献
Therapeutic efficacy of a probiotic preparation on idiopathic halitosis: a retrospective observational study. Halitosis in oral lichen planus patients. Effectiveness of a combination of laccase and green coffee extract on oral malodor: A comparative, randomized, controlled, evaluator-blind, parallel-group trial. Validation of a sensor system for the measurement of breath ammonia using selected-ion flow-tube mass spectrometry. Identifying viral infections through analysis of head space volatile organic compounds.
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