{"title":"一组原发性斯约格伦综合征患者的外周 B 细胞亚群特征及其潜在临床意义","authors":"Yixiao Xing , Boya Li , Pan Wei , Hong Hua","doi":"10.1016/j.jds.2023.12.024","DOIUrl":null,"url":null,"abstract":"<div><h3>Background/purpose</h3><p>Primary Sjögren's syndrome is a prototypical autoimmune disease, with B cell dysfunction as a dominant feature. Further insights into distribution of B cell subsets in primary Sjögren's syndrome are urgently required to identify the most appropriate target subpopulation. We aimed to evaluate the profiles of B lymphocyte subpopulations in primary Sjögren's syndrome patients and to investigate their clinical significance.</p></div><div><h3>Materials and methods</h3><p>Thirty primary Sjögren's syndrome patients and 15 age-and sex-matched healthy controls were recruited. Peripheral B cell subsets were analyzed by flow cytometry.</p></div><div><h3>Results</h3><p>Compared to healthy controls, circulating CD19<sup>+</sup> B cells, CD19<sup>+</sup>CD20<sup>−</sup> B cells, CD19<sup>+</sup>CD27<sup>−</sup>IgD<sup>+</sup> naïve B cells, CD19<sup>+</sup>IgD<sup>+</sup>CD38<sup>high</sup> plasmablasts, CD19<sup>+</sup>CD24<sup>high</sup>CD38<sup>high</sup> transitional B cells and CD19<sup>+</sup>CD20<sup>−</sup>CD27<sup>+</sup>CD38<sup>+</sup> plasma cells were elevated in patients with primary Sjögren's syndrome, whereas CD19<sup>+</sup>CD27<sup>+</sup> memory B cells, CD19<sup>+</sup>CD27<sup>−</sup>IgD<sup>-</sup> double negative B cells and CD19<sup>+</sup>CD24<sup>hi</sup>CD27<sup>+</sup> Bregs were decreased. Furthermore, the percentage of circulating CD19<sup>+</sup>CD20<sup>−</sup>CD27<sup>+</sup>CD38<sup>+</sup> plasma cells was positively correlated with serum IgG levels and the proportional area of lymphocytic infiltration of labial gland.</p></div><div><h3>Conclusion</h3><p>We identified a comprehensive B lymphocyte subset distribution profile in primary Sjögren's syndrome. Moreover, we detected a clinical significance of CD19<sup>+</sup>CD20<sup>−</sup>CD27<sup>+</sup>CD38<sup>+</sup> plasma cells, suggesting that these cells might play a key role in disease pathology and represent potential therapeutic targets.</p></div>","PeriodicalId":15583,"journal":{"name":"Journal of Dental Sciences","volume":"19 3","pages":"Pages 1554-1563"},"PeriodicalIF":3.4000,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1991790223004166/pdfft?md5=a27ddb6b5108483d288112da873c44aa&pid=1-s2.0-S1991790223004166-main.pdf","citationCount":"0","resultStr":"{\"title\":\"Profiles of peripheral B cell subsets in a cohort of primary Sjögren's syndrome patients and their potential clinical significance\",\"authors\":\"Yixiao Xing , Boya Li , Pan Wei , Hong Hua\",\"doi\":\"10.1016/j.jds.2023.12.024\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Background/purpose</h3><p>Primary Sjögren's syndrome is a prototypical autoimmune disease, with B cell dysfunction as a dominant feature. Further insights into distribution of B cell subsets in primary Sjögren's syndrome are urgently required to identify the most appropriate target subpopulation. We aimed to evaluate the profiles of B lymphocyte subpopulations in primary Sjögren's syndrome patients and to investigate their clinical significance.</p></div><div><h3>Materials and methods</h3><p>Thirty primary Sjögren's syndrome patients and 15 age-and sex-matched healthy controls were recruited. Peripheral B cell subsets were analyzed by flow cytometry.</p></div><div><h3>Results</h3><p>Compared to healthy controls, circulating CD19<sup>+</sup> B cells, CD19<sup>+</sup>CD20<sup>−</sup> B cells, CD19<sup>+</sup>CD27<sup>−</sup>IgD<sup>+</sup> naïve B cells, CD19<sup>+</sup>IgD<sup>+</sup>CD38<sup>high</sup> plasmablasts, CD19<sup>+</sup>CD24<sup>high</sup>CD38<sup>high</sup> transitional B cells and CD19<sup>+</sup>CD20<sup>−</sup>CD27<sup>+</sup>CD38<sup>+</sup> plasma cells were elevated in patients with primary Sjögren's syndrome, whereas CD19<sup>+</sup>CD27<sup>+</sup> memory B cells, CD19<sup>+</sup>CD27<sup>−</sup>IgD<sup>-</sup> double negative B cells and CD19<sup>+</sup>CD24<sup>hi</sup>CD27<sup>+</sup> Bregs were decreased. Furthermore, the percentage of circulating CD19<sup>+</sup>CD20<sup>−</sup>CD27<sup>+</sup>CD38<sup>+</sup> plasma cells was positively correlated with serum IgG levels and the proportional area of lymphocytic infiltration of labial gland.</p></div><div><h3>Conclusion</h3><p>We identified a comprehensive B lymphocyte subset distribution profile in primary Sjögren's syndrome. Moreover, we detected a clinical significance of CD19<sup>+</sup>CD20<sup>−</sup>CD27<sup>+</sup>CD38<sup>+</sup> plasma cells, suggesting that these cells might play a key role in disease pathology and represent potential therapeutic targets.</p></div>\",\"PeriodicalId\":15583,\"journal\":{\"name\":\"Journal of Dental Sciences\",\"volume\":\"19 3\",\"pages\":\"Pages 1554-1563\"},\"PeriodicalIF\":3.4000,\"publicationDate\":\"2024-07-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S1991790223004166/pdfft?md5=a27ddb6b5108483d288112da873c44aa&pid=1-s2.0-S1991790223004166-main.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Dental Sciences\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1991790223004166\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"DENTISTRY, ORAL SURGERY & MEDICINE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Dental Sciences","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1991790223004166","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"DENTISTRY, ORAL SURGERY & MEDICINE","Score":null,"Total":0}
引用次数: 0
摘要
背景/目的原发性斯约格伦综合征是一种典型的自身免疫性疾病,以 B 细胞功能障碍为主要特征。我们迫切需要进一步了解原发性斯约格伦综合征中 B 细胞亚群的分布情况,以确定最合适的目标亚群。我们的目的是评估原发性斯约格伦综合征患者 B 淋巴细胞亚群的分布情况,并研究其临床意义。流式细胞术分析了外周 B 细胞亚群。而 CD19+CD27+ 记忆 B 细胞、CD19+CD27-IgD- 双阴性 B 细胞和 CD19+CD24hiCD27+ Bregs 则减少。此外,循环中 CD19+CD20-CD27+CD38+ 浆细胞的百分比与血清 IgG 水平和唇腺淋巴细胞浸润的比例面积呈正相关。此外,我们还发现了 CD19+CD20-CD27+CD38+ 浆细胞的临床意义,这表明这些细胞可能在疾病病理过程中起着关键作用,是潜在的治疗靶点。
Profiles of peripheral B cell subsets in a cohort of primary Sjögren's syndrome patients and their potential clinical significance
Background/purpose
Primary Sjögren's syndrome is a prototypical autoimmune disease, with B cell dysfunction as a dominant feature. Further insights into distribution of B cell subsets in primary Sjögren's syndrome are urgently required to identify the most appropriate target subpopulation. We aimed to evaluate the profiles of B lymphocyte subpopulations in primary Sjögren's syndrome patients and to investigate their clinical significance.
Materials and methods
Thirty primary Sjögren's syndrome patients and 15 age-and sex-matched healthy controls were recruited. Peripheral B cell subsets were analyzed by flow cytometry.
Results
Compared to healthy controls, circulating CD19+ B cells, CD19+CD20− B cells, CD19+CD27−IgD+ naïve B cells, CD19+IgD+CD38high plasmablasts, CD19+CD24highCD38high transitional B cells and CD19+CD20−CD27+CD38+ plasma cells were elevated in patients with primary Sjögren's syndrome, whereas CD19+CD27+ memory B cells, CD19+CD27−IgD- double negative B cells and CD19+CD24hiCD27+ Bregs were decreased. Furthermore, the percentage of circulating CD19+CD20−CD27+CD38+ plasma cells was positively correlated with serum IgG levels and the proportional area of lymphocytic infiltration of labial gland.
Conclusion
We identified a comprehensive B lymphocyte subset distribution profile in primary Sjögren's syndrome. Moreover, we detected a clinical significance of CD19+CD20−CD27+CD38+ plasma cells, suggesting that these cells might play a key role in disease pathology and represent potential therapeutic targets.
期刊介绍:
he Journal of Dental Sciences (JDS), published quarterly, is the official and open access publication of the Association for Dental Sciences of the Republic of China (ADS-ROC). The precedent journal of the JDS is the Chinese Dental Journal (CDJ) which had already been covered by MEDLINE in 1988. As the CDJ continued to prove its importance in the region, the ADS-ROC decided to move to the international community by publishing an English journal. Hence, the birth of the JDS in 2006. The JDS is indexed in the SCI Expanded since 2008. It is also indexed in Scopus, and EMCare, ScienceDirect, SIIC Data Bases.
The topics covered by the JDS include all fields of basic and clinical dentistry. Some manuscripts focusing on the study of certain endemic diseases such as dental caries and periodontal diseases in particular regions of any country as well as oral pre-cancers, oral cancers, and oral submucous fibrosis related to betel nut chewing habit are also considered for publication. Besides, the JDS also publishes articles about the efficacy of a new treatment modality on oral verrucous hyperplasia or early oral squamous cell carcinoma.