{"title":"生长激素直接刺激 GATA2 的表达","authors":"Mana Mitsutani , Midori Matsushita , Mei Yokoyama , Ayumu Morita , Hiromi Hano , Tomomi Fujikawa , Tetsuya Tagami , Kenji Moriyama","doi":"10.1016/j.ghir.2024.101572","DOIUrl":null,"url":null,"abstract":"<div><h3>Objective</h3><p><span>GATA2 is a key transcription factor involved in the differentiation and determination of thyrotrophs and </span>gonadotrophs<span> in pituitary and hematopoietic development. However, studies on the upstream ligands of the GATA2 signal transduction pathway have been limited. To identify upstream ligands, we examined growth hormone (GH) as a plausible stimulator.</span></p></div><div><h3>Design</h3><p>We evaluated GH-induced GATA2 expression in murine TtT/GF thyrotrophic pituitary tumor<span> cells and its direct impact on the GHR/JAK/STAT5 pathway using a combination of a reporter assay, real-time quantitative polymerase chain reaction, and western blotting.</span></p></div><div><h3>Results</h3><p>GATA2 expression increased with activated STAT5B<span> in a dose-dependent manner and was inhibited by a STAT5 specific inhibitor. Moreover, we found functional STAT5B binding site consensus sequences at −359 bp in the GATA2 promoter region.</span></p></div><div><h3>Conclusion</h3><p>These findings suggest that GH directly stimulates GATA2 via the GHR/JAK/STAT pathway and participates in various developmental phenomena mediated by GATA2.</p></div>","PeriodicalId":12803,"journal":{"name":"Growth Hormone & Igf Research","volume":null,"pages":null},"PeriodicalIF":1.6000,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Growth hormone directly stimulates GATA2 expression\",\"authors\":\"Mana Mitsutani , Midori Matsushita , Mei Yokoyama , Ayumu Morita , Hiromi Hano , Tomomi Fujikawa , Tetsuya Tagami , Kenji Moriyama\",\"doi\":\"10.1016/j.ghir.2024.101572\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Objective</h3><p><span>GATA2 is a key transcription factor involved in the differentiation and determination of thyrotrophs and </span>gonadotrophs<span> in pituitary and hematopoietic development. However, studies on the upstream ligands of the GATA2 signal transduction pathway have been limited. To identify upstream ligands, we examined growth hormone (GH) as a plausible stimulator.</span></p></div><div><h3>Design</h3><p>We evaluated GH-induced GATA2 expression in murine TtT/GF thyrotrophic pituitary tumor<span> cells and its direct impact on the GHR/JAK/STAT5 pathway using a combination of a reporter assay, real-time quantitative polymerase chain reaction, and western blotting.</span></p></div><div><h3>Results</h3><p>GATA2 expression increased with activated STAT5B<span> in a dose-dependent manner and was inhibited by a STAT5 specific inhibitor. Moreover, we found functional STAT5B binding site consensus sequences at −359 bp in the GATA2 promoter region.</span></p></div><div><h3>Conclusion</h3><p>These findings suggest that GH directly stimulates GATA2 via the GHR/JAK/STAT pathway and participates in various developmental phenomena mediated by GATA2.</p></div>\",\"PeriodicalId\":12803,\"journal\":{\"name\":\"Growth Hormone & Igf Research\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.6000,\"publicationDate\":\"2024-02-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Growth Hormone & Igf Research\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1096637424000029\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"CELL BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Growth Hormone & Igf Research","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1096637424000029","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"CELL BIOLOGY","Score":null,"Total":0}
引用次数: 0
摘要
目的GATA2是一种关键的转录因子，参与垂体和造血发育过程中甲状腺和促性腺激素的分化和决定。然而，有关 GATA2 信号转导通路上游配体的研究还很有限。为了确定上游配体，我们将生长激素（GH）作为一种可能的刺激物进行了研究。结果GATA2的表达以剂量依赖的方式随活化的STAT5B而增加，并受到STAT5特异性抑制剂的抑制。此外，我们在 GATA2 启动子区域的 -359 bp 处发现了功能性 STAT5B 结合位点共识序列。
GATA2 is a key transcription factor involved in the differentiation and determination of thyrotrophs and gonadotrophs in pituitary and hematopoietic development. However, studies on the upstream ligands of the GATA2 signal transduction pathway have been limited. To identify upstream ligands, we examined growth hormone (GH) as a plausible stimulator.
Design
We evaluated GH-induced GATA2 expression in murine TtT/GF thyrotrophic pituitary tumor cells and its direct impact on the GHR/JAK/STAT5 pathway using a combination of a reporter assay, real-time quantitative polymerase chain reaction, and western blotting.
Results
GATA2 expression increased with activated STAT5B in a dose-dependent manner and was inhibited by a STAT5 specific inhibitor. Moreover, we found functional STAT5B binding site consensus sequences at −359 bp in the GATA2 promoter region.
Conclusion
These findings suggest that GH directly stimulates GATA2 via the GHR/JAK/STAT pathway and participates in various developmental phenomena mediated by GATA2.
期刊介绍：
Growth Hormone & IGF Research is a forum for research on the regulation of growth and metabolism in humans, animals, tissues and cells. It publishes articles on all aspects of growth-promoting and growth-inhibiting hormones and factors, with particular emphasis on insulin-like growth factors (IGFs) and growth hormone. This reflects the increasing importance of growth hormone and IGFs in clinical medicine and in the treatment of diseases.
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