Wu-Shuang Guo, Xin Deng, Man-Xin Yang, Tian Hu, Xing-Han Li
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引用次数: 0
摘要
本研究旨在调查小鼠牙槽骨中昼夜节律钟基因的表达情况,以及这些变化的可能原因。给 50 只 C57 小鼠口服牙龈脓疱病菌,建立牙周炎模型,以健康小鼠为对照。对两组小鼠的牙槽骨进行微型计算机断层扫描,测量附着丧失量,并通过实时荧光定量聚合酶链反应(qRT-PCR)检测各时钟基因和牙周炎相关炎症因子 mRNA 的相对表达量。小鼠模型建立后,牙周炎组牙槽骨高度明显低于正常组(p p ≤ 0.05),牙周炎组昼夜节律消失,转录水平特征发生变化。与正常组相比,牙周炎组白细胞介素(IL)-6、肿瘤坏死因子-α(TNF-α)和干扰素(IFN-γ)mRNA转录水平升高。综上所述,正常小鼠牙槽骨中Bmal1、Per2和Cry1的mRNA转录水平具有昼夜节律性,但在牙周炎情况下该节律消失,其发生原因可能与炎性细胞因子有关。
A pilot study on the expression of circadian clock genes in the alveolar bone of mice with periodontitis.
This study aimed to investigate the expression of circadian clock genes in mouse alveolar bone, and the possible reasons for these changes. Fifty C57 mice were orally inoculated with P. gingivalis, establishing a model of periodontitis using healthy mice as controls. The alveolar bone of both groups was taken for micro-computed tomography scanning to measure the amount of attachment loss, and the relative expression of mRNA in each clock gene and periodontitis related inflammatory factor was detected by real-time fluorescence quantitative polymerase chain reaction (qRT-PCR). After the establishment of the mouse model, the height of alveolar bone in the periodontitis group was significantly lower than that in the normal group (p < 0.05). The relative transcriptional level of Bmal1, Per2, and Cry1 mRNA was in the circadian rhythm in the normal group (p ≤ 0.05), while in the periodontitis group, its circadian rhythm disappeared and the transcriptional level characteristics were changed. Interleukin (IL)-6, tumor necrosis factor-alpha (TNF-α), and interferon (IFN-γ) mRNA transcriptional level were elevated in the periodontitis group compared to the normal group. In conclusion, the mRNA transcriptional level of Bmal1, Per2, and Cry1 in alveolar bone of normal mice has circadian rhythm, but the rhythm disappears under the condition of periodontitis, and the cause of its occurrence may be related to inflammatory cytokines.
期刊介绍:
Chronobiology International is the journal of biological and medical rhythm research. It is a transdisciplinary journal focusing on biological rhythm phenomena of all life forms. The journal publishes groundbreaking articles plus authoritative review papers, short communications of work in progress, case studies, and letters to the editor, for example, on genetic and molecular mechanisms of insect, animal and human biological timekeeping, including melatonin and pineal gland rhythms. It also publishes applied topics, for example, shiftwork, chronotypes, and associated personality traits; chronobiology and chronotherapy of sleep, cardiovascular, pulmonary, psychiatric, and other medical conditions. Articles in the journal pertain to basic and applied chronobiology, and to methods, statistics, and instrumentation for biological rhythm study.
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