牙周炎中的 Gli1+ 牙周间充质干细胞

Journal of dental research Pub Date : 2024-03-01 Epub Date: 2024-01-29 DOI:10.1177/00220345231220915
Y Deng, Q Li, K K H Svoboda, L A Opperman, L B Ruest, X Liu
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摘要

牙周间充质干细胞(MSCs)在维持牙周稳态和组织修复方面发挥着至关重要的作用。然而,人们对牙周间充质干细胞在牙周疾病条件下的体内反应知之甚少,这给牙周组织再生带来了挑战。在这项研究中,Gli1被用作牙周间充质干细胞的标记物,并结合Gli1-cre ERT2小鼠模型进行系谱追踪,以研究诱导性牙周炎模型中牙周间充质干细胞的命运。我们的研究结果表明,在发炎的牙周中,Gli1+间充质干细胞的数量和贡献率发生了重大变化。在牙周炎诱导的牙齿中,促进牙周韧带平衡的 Gli1+ 间充质干细胞数量减少。虽然 Gli1+ 间充质干细胞的增殖在牙周炎组和对照组之间没有显著差异,但在病变牙中有更多的 Gli1+ 间充质干细胞发生凋亡。此外,随着牙周炎的发展,用于成骨分化的 Gli1+ 间充质干细胞数量减少。拔牙后,Gli1+间充质干细胞对牙周炎引起的牙槽骨修复的贡献明显减少。总之,这些研究结果表明,Gli1+间充质干细胞在牙周炎中的功能受到损害,包括对牙周稳态的贡献减少和损伤反应受损。
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Gli1+ Periodontal Mesenchymal Stem Cells in Periodontitis.

Periodontal mesenchymal stem cells (MSCs) play a crucial role in maintaining periodontium homeostasis and in tissue repair. However, little is known about how periodontal MSCs in vivo respond under periodontal disease conditions, posing a challenge for periodontium tissue regeneration. In this study, Gli1 was used as a periodontal MSC marker and combined with a Gli1-cre ERT2 mouse model for lineage tracing to investigate periodontal MSC fate in an induced periodontitis model. Our findings show significant changes in the number and contribution of Gli1+ MSCs within the inflamed periodontium. The number of Gli1+ MSCs that contributed to periodontal ligament homeostasis decreased in the periodontitis-induced teeth. While the proliferation of Gli1+ MSCs had no significant difference between the periodontitis and the control groups, more Gli1+ MSCs underwent apoptosis in diseased teeth. In addition, the number of Gli1+ MSCs for osteogenic differentiation decreased during the progression of periodontitis. Following tooth extraction, the contribution of Gli1+ MSCs to the tooth socket repair was significantly reduced in the periodontitis-induced teeth. Collectively, these findings indicate that the function of Gli1+ MSCs in periodontitis was compromised, including reduced contribution to periodontium homeostasis and impaired injury response.

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