噬菌体葡萄球菌对感染性皮肤伤口模型的抗菌活性研究

G. N. Genatullina
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引用次数: 0

摘要

本研究的目的是研究噬菌体对受金黄色葡萄球菌感染的皮肤伤口模型的抗菌活性。抗菌活性的研究是通过在非线性雄性大鼠肩胛间区建立感染了金黄色葡萄球菌的皮肤伤口模型进行的。对动物的所有操作都是按照实验室动物管理和工作监管文件的要求进行的。研究结果表明,噬菌体葡萄球菌在伤口感染情况下外用具有明显的抗菌活性,伤口分泌物的细菌污染程度降低以及炎症现象减少证实了这一点,局部的炎症现象以充血减少的形式出现,而全身的炎症现象则以 C 反应蛋白水平、白细胞总数以及白细胞炎症指数降低的形式出现。已确定的抗菌效果可与外用消毒药物--0.05% 的洗必泰葡萄糖酸溶液相媲美,但在评估微生物污染时,发现噬菌体表现出更明显的抗菌活性。因此,在金黄色葡萄球菌感染的皮肤伤口模型中,噬菌体表现出了明显的抗菌活性,伤口分泌物中微生物菌落数量的减少以及炎症严重程度的减轻都证实了这一点。
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Study of the Antimicrobial Activity of Staphylococcal Bacteriophage on a Model of an Infected Skin Wound
The purpose of this study was to investigate the antimicrobial activity of staphylococcal bacteriophage on a model of a skin wound infected with Staphylococcus aureus. The study of antimicrobial activity was carried out in vivo by experimental modeling of a skin wound infected with S.aureus in the interscapular region in nonlinear male rats. All manipulations with animals were performed in accordance with the requirements of regulatory documentation overseeing the management of laboratory animals and work with them. The results obtained indicate the pronounced antimicrobial activity of staphylococcal bacteriophage in conditions of wound infection when applied externally, which is confirmed by a decrease in the degree of bacterial contamination of wound discharge, as well as a decrease in inflammatory phenomena, both at the local level in the form of a decrease in hyperemia, and generalized in the form of a decrease in C-reactive protein levels, total leukocyte count, as well as a decrease in the leukocyte inflammatory index. The identified antimicrobial effect is comparable to the external use of an antiseptic drug — a solution of chlorhexidine digluconate 0.05%, however, when assessing microbial contamination, it was found that the bacteriophage exhibits more pronounced antibacterial activity. Thus, the staphylococcal bacteriophage showed pronounced antimicrobial activity in a model of a skin wound infected with S.aureus, confirmed by a decrease in the number of colonies of the microorganism in the wound discharge, as well as a decrease in the severity of inflammation.
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