初筛晒干肉类中产生霉菌毒素的真菌分离物

Christiana N. Opara, F. I. Omeje
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摘要

本 研 究 旨 在 筛 选 从 晒 干 肉 类 样 本 中 分 离 出 的 真 菌 , 以 确 定 晒 干 肉 类 中 可 能 产 生 霉 菌 毒 素 的 真 菌 。研 究 从 晒 干 肉 类 样 本 中 共 分 离 出 8 种 真 菌 。从 八 个 晒 干 肉 类 样 本 分 离 出 的 真 菌 会 在 马 铃 薯 葡 萄 糖 琼 脂 和 沙 保 利 葡 萄 糖 琼 脂 上 分 别 培 养 , 以 取 得 纯 菌 落 作 进 一 步 分 析 。使用椰子琼脂培养基(CAM)对晒干肉样品中的真菌毒素进行检测。结果显示,一些真菌分离物具有产生霉菌毒素的能力。从霉菌毒素筛选结果来看,只有四(4)种真菌(曲霉属和青霉属)呈阳性(+),而根瘤菌属、镰刀菌属和粘菌属呈阴性(-)。不同市场的霉菌毒素污染水平各不相同,其中学院市场的霉菌毒素污染水平最高(71.4%),其次是奥杰市场(14.3%)和奥雷米吉市场(14.3%)。晒干肉样品中产生霉菌毒素的真菌和霉菌毒素含量是公共卫生问题。此外,这些结果还要求提高对晒干肉安全性的认识。
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Primary Screening for Fungi Isolates that Produce Mycotoxin from Sun-Dried Meat
This study aims to screen fungal isolates from sun dried meat samples to identify fungal presumed producing mycotoxins in the sun dried meat. A total of 8 fungal isolates from sun dried meat were used for the study. They are Aspergillus (2), Penicillium spp (3), Fusarium spp, Mucor spp and Rhizopus spp. The fungal isolates from eight sun dried-meat samples were sub-cultured on Potato Dextrose Agar and Sabouraud Dextrose agar in order to obtain pure colonies for further analysis. The detection of mycotoxins in the sun dry meat samples was carried out by the use of Coconut Agar Media (CAM), by which the fungal isolates were cultured and plates incubated for 3-7days at 28C. The results obtained show that some of the fungal isolates had the ability to produce mycotoxins. From the mycotoxin screening, only four (4) species (Aspergillus spps, and Penicillium spp) were positive (+) and Rhizopus spp, Fusarium spp and Mucor spp, were negative (-). Mycotoxin contamination level varied in different market locations in which Academy Market had the highest level of mycotoxin (71.4%) followed by Oje Market (14.3%) and Oremiji Market (14.3%). Mycotoxigenic producing fungi and mycotoxin levels in the sun dried meat samples are public health concern. Also these results calls for more sensitization for safety of sun dried meat.
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