P-TDHM：开源便携式远心数字全息显微镜

IF 2 Q3 ENGINEERING, ELECTRICAL & ELECTRONIC HardwareX Pub Date : 2024-01-26 DOI:10.1016/j.ohx.2024.e00508

Lei Jin , Ziyang Yu , Aaron Au , Peter Serles , Nan Wang , Jeremy T. Lant , Tobin Filleter , Christopher M. Yip

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引用次数: 0

摘要

我们介绍了一种利用现成组件设计的低成本便携式远心数字全息显微镜（P-TDHM）。我们介绍了该系统的硬件和软件要素，并通过在线和离轴模式对从纳米打印目标到活体 HeLa 细胞、HEK293 细胞和 Dolichospermum 等样品进行成像，评估了该系统的性能。我们的研究结果表明，该系统能以小巧的外形获取高质量的定量相位图像，具有纳米级的轴向分辨率和亚微米级的横向分辨率，使其成为资源有限环境和偏远地区的理想选择。我们的设计标志着在使远心数字全息显微技术为更广泛的群体所接受和负担得起方面迈出了重要一步。

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P-TDHM: Open-source portable telecentric digital holographic microscope

We present the design of a low-cost, portable telecentric digital holographic microscope (P-TDHM) that utilizes off-the-shelf components. We describe the system’s hardware and software elements and evaluate its performance by imaging samples ranging from nano-printed targets to live HeLa cells, HEK293 cells, and Dolichospermum via both in-line and off-axis modes. Our results demonstrate that the system can acquire high quality quantitative phase images with nanometer axial and sub-micron lateral resolution in a small form factor, making it a promising candidate for resource-limited settings and remote locations. Our design represents a significant step forward in making telecentric digital holographic microscopy accessible and affordable to the broader community.

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来源期刊

HardwareX Engineering-Industrial and Manufacturing Engineering

CiteScore

4.10

自引率

18.20%

发文量

124

审稿时长

24 weeks

期刊介绍： HardwareX is an open access journal established to promote free and open source designing, building and customizing of scientific infrastructure (hardware). HardwareX aims to recognize researchers for the time and effort in developing scientific infrastructure while providing end-users with sufficient information to replicate and validate the advances presented. HardwareX is open to input from all scientific, technological and medical disciplines. Scientific infrastructure will be interpreted in the broadest sense. Including hardware modifications to existing infrastructure, sensors and tools that perform measurements and other functions outside of the traditional lab setting (such as wearables, air/water quality sensors, and low cost alternatives to existing tools), and the creation of wholly new tools for either standard or novel laboratory tasks. Authors are encouraged to submit hardware developments that address all aspects of science, not only the final measurement, for example, enhancements in sample preparation and handling, user safety, and quality control. The use of distributed digital manufacturing strategies (e.g. 3-D printing) is encouraged. All designs must be submitted under an open hardware license.