终点线上的代谢洞察:通过呼吸挥发性有机化合物分析解读超级马拉松运动员的生理变化。

IF 3.7 4区 医学 Q1 BIOCHEMICAL RESEARCH METHODS Journal of breath research Pub Date : 2024-02-12 DOI:10.1088/1752-7163/ad23f5
Hsuan Chou, Kayleigh Arthur, Elen Shaw, Chad Schaber, Billy Boyle, Max Allsworth, Eli F Kelley, Glenn M Stewart, Courtney M Wheatley, Jesse Schwartz, Caitlin C Fermoyle, Briana L Ziegler, Kay A Johnson, Paul Robach, Patrick Basset, Bruce D Johnson
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引用次数: 0

摘要

剧烈运动可在肺部和人体其他部位引起独特的生理反应。呼出气体中的挥发性有机化合物(VOCs)非常适合研究剧烈运动对肺部的影响,因为呼出气体的基质非常接近呼吸道。由于呼出气体中的挥发性有机化合物可能来自血液,因此其含量的变化也应能显示疲惫运动对人体产生的更广泛的生理影响。目前,有关剧烈运动对呼吸挥发性有机化合物影响的公开数据还很有限。呼吸具有很大的生物标记分析潜力,因为它可以非侵入性地收集,并捕捉实时的代谢变化,从而更好地了解疲惫运动的影响。在这项研究中,我们收集了一小部分参加 2019 年勃朗峰超级马拉松赛(UTMB)的精英选手的呼吸样本。最终分析包括 24 名受试者在赛前和赛后采集的配对样本。所有 48 份样本均使用带有 GC-Orbitrap™ 的呼吸活检平台,通过热脱附气相色谱-质谱法(TD-GC-MS)进行分析。Wilcoxon 符号秩检验用于确定赛前和赛后呼气样本中挥发性有机化合物丰度是否存在差异(调整后 p < 0.05)。我们在精英选手的呼气样本中总共鉴定出 793 种挥发性有机化合物。其中,63 种挥发性有机化合物在校正多重测试后显示出赛前和赛后样本之间的显著差异(12 种减少,51 种增加)。所发现的特定挥发性有机化合物表明,在剧烈运动时,脂肪酸氧化、炎症和肠道微生物组活动可能会发生改变。这项研究表明,剧烈运动会导致挥发性有机化合物丰度发生重大变化。进一步研究挥发性有机化合物的变化以及其他生理测量指标,有助于我们更好地了解剧烈运动对身体的影响以及呼出气体中挥发性有机化合物的差异。
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Metabolic insights at the finish line: deciphering physiological changes in ultramarathon runners through breath VOC analysis.

Exhaustive exercise can induce unique physiological responses in the lungs and other parts of the human body. The volatile organic compounds (VOCs) in exhaled breath are ideal for studying the effects of exhaustive exercise on the lungs due to the proximity of the breath matrix to the respiratory tract. As breath VOCs can originate from the bloodstream, changes in abundance should also indicate broader physiological effects of exhaustive exercise on the body. Currently, there is limited published data on the effects of exhaustive exercise on breath VOCs. Breath has great potential for biomarker analysis as it can be collected non-invasively, and capture real-time metabolic changes to better understand the effects of exhaustive exercise. In this study, we collected breath samples from a small group of elite runners participating in the 2019 Ultra-Trail du Mont Blanc ultra-marathon. The final analysis included matched paired samples collected before and after the race from 24 subjects. All 48 samples were analyzed using the Breath Biopsy Platform with GC-Orbitrap™ via thermal desorption gas chromatography-mass spectrometry. The Wilcoxon signed-rank test was used to determine whether VOC abundances differed between pre- and post-race breath samples (adjustedP-value < .05). We identified a total of 793 VOCs in the breath samples of elite runners. Of these, 63 showed significant differences between pre- and post-race samples after correction for multiple testing (12 decreased, 51 increased). The specific VOCs identified suggest the involvement of fatty acid oxidation, inflammation, and possible altered gut microbiome activity in response to exhaustive exercise. This study demonstrates significant changes in VOC abundance resulting from exhaustive exercise. Further investigation of VOC changes along with other physiological measurements can help improve our understanding of the effect of exhaustive exercise on the body and subsequent differences in VOCs in exhaled breath.

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来源期刊
Journal of breath research
Journal of breath research BIOCHEMICAL RESEARCH METHODS-RESPIRATORY SYSTEM
CiteScore
7.60
自引率
21.10%
发文量
49
审稿时长
>12 weeks
期刊介绍: Journal of Breath Research is dedicated to all aspects of scientific breath research. The traditional focus is on analysis of volatile compounds and aerosols in exhaled breath for the investigation of exogenous exposures, metabolism, toxicology, health status and the diagnosis of disease and breath odours. The journal also welcomes other breath-related topics. Typical areas of interest include: Big laboratory instrumentation: describing new state-of-the-art analytical instrumentation capable of performing high-resolution discovery and targeted breath research; exploiting complex technologies drawn from other areas of biochemistry and genetics for breath research. Engineering solutions: developing new breath sampling technologies for condensate and aerosols, for chemical and optical sensors, for extraction and sample preparation methods, for automation and standardization, and for multiplex analyses to preserve the breath matrix and facilitating analytical throughput. Measure exhaled constituents (e.g. CO2, acetone, isoprene) as markers of human presence or mitigate such contaminants in enclosed environments. Human and animal in vivo studies: decoding the ''breath exposome'', implementing exposure and intervention studies, performing cross-sectional and case-control research, assaying immune and inflammatory response, and testing mammalian host response to infections and exogenous exposures to develop information directly applicable to systems biology. Studying inhalation toxicology; inhaled breath as a source of internal dose; resultant blood, breath and urinary biomarkers linked to inhalation pathway. Cellular and molecular level in vitro studies. Clinical, pharmacological and forensic applications. Mathematical, statistical and graphical data interpretation.
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