Christian Jünger, Frank Imkamp, Suraj Balakrishna, Marina Gysin, Klara Haldimann, Silvio D Brugger, Thomas C Scheier, Benjamin Hampel, Sven N Hobbie, Huldrych F Günthard, Dominique L Braun
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Bacterial isolates were subjected to whole genome sequencing (WGS).</p><p><strong>Results: </strong>Of 142 participants, 141 (99%) were MSM and 118 (84%) living with HIV. Participants were treated with ceftriaxone (<i>N</i> = 79), azithromycin (<i>N</i> = 2), or a combination of both (<i>N</i> = 61). No clinical or microbiological failures were observed. From 182 positive PCR samples taken, 23 were available for detailed analysis. Based on minimal inhibitory concentrations (MICs), all isolates were susceptible to ceftriaxone, gentamicin, cefixime, cefpodoxime, ertapenem, zoliflodacin, and spectinomycin. Resistance to azithromycin, tetracyclines and ciprofloxacin was observed in 10 (43%), 23 (100%) and 11 (48%) of the cases, respectively. Analysis of WGS data revealed combinations of resistance determinants that matched with the corresponding phenotypic resistance pattern of each isolate.</p><p><strong>Conclusion: </strong>Among the MSM diagnosed with NG mainly acquired in Switzerland, ceftriaxone MICs were low for a subset of bacterial isolates studied and no treatment failures were observed. For azithromycin, high occurrences of in vitro resistance were found. Gentamicin, cefixime, cefpodoxime, ertapenem, spectinomycin, and zoliflodacin displayed excellent in vitro activity against the 23 isolates underscoring their potential as alternative agents to ceftriaxone.</p>","PeriodicalId":14408,"journal":{"name":"International Journal of STD & AIDS","volume":null,"pages":null},"PeriodicalIF":1.4000,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Phenotypic and genotypic characterization of <i>Neisseria gonorrhoeae</i> isolates among individuals at high risk for sexually transmitted diseases in Zurich, Switzerland.\",\"authors\":\"Christian Jünger, Frank Imkamp, Suraj Balakrishna, Marina Gysin, Klara Haldimann, Silvio D Brugger, Thomas C Scheier, Benjamin Hampel, Sven N Hobbie, Huldrych F Günthard, Dominique L Braun\",\"doi\":\"10.1177/09564624241230266\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>While ceftriaxone resistance remains scarce in Switzerland, global <i>Neisseria gonorrhoeae</i> (NG) antimicrobial resistance poses an urgent threat. 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引用次数: 0
摘要
背景:尽管头孢曲松耐药性在瑞士仍然很少见,但全球淋病奈瑟菌(NG)的抗菌药耐药性构成了紧迫的威胁。本研究描述了确诊感染 NG 的 MSM(男男性行为者)的临床特征,并通过表型和基因型方法分析了 NG 耐药性:方法:在2019年1月至2021年12月期间,对三个临床队列中NG聚合酶链反应检测(PCR)呈阳性的MSM入组数据进行了分析,并将其与抗生素药敏试验联系起来。细菌分离物进行了全基因组测序(WGS):在 142 名参与者中,141 人(99%)为 MSM,118 人(84%)为 HIV 感染者。参与者接受了头孢曲松(79 人)、阿奇霉素(2 人)或两者联合治疗(61 人)。未发现临床或微生物学治疗失败。在采集的 182 份 PCR 阳性样本中,有 23 份可供详细分析。根据最小抑菌浓度(MIC),所有分离菌株都对头孢曲松、庆大霉素、头孢克肟、头孢泊肟、厄他培南、唑氟达星和广谱霉素敏感。对阿奇霉素、四环素和环丙沙星耐药的病例分别有 10 例(43%)、23 例(100%)和 11 例(48%)。对 WGS 数据的分析表明,耐药性决定因素的组合与每个分离株的相应表型耐药性模式相匹配:结论:在主要于瑞士感染 NG 的 MSM 诊断病例中,头孢曲松对部分细菌分离物的 MIC 值较低,未发现治疗失败的病例。阿奇霉素的体外耐药性发生率较高。庆大霉素、头孢克肟、头孢泊肟、厄他培南、光谱霉素和唑来氟哌酸对 23 个分离菌株显示出极佳的体外活性,突出表明它们有可能成为头孢曲松的替代药物。
Phenotypic and genotypic characterization of Neisseria gonorrhoeae isolates among individuals at high risk for sexually transmitted diseases in Zurich, Switzerland.
Background: While ceftriaxone resistance remains scarce in Switzerland, global Neisseria gonorrhoeae (NG) antimicrobial resistance poses an urgent threat. This study describes clinical characteristics in MSM (men who have sex with men) diagnosed with NG infection and analyses NG resistance by phenotypic and genotypic means.
Methods: Data of MSM enrolled in three clinical cohorts with a positive polymerase chain reaction test (PCR) for NG were analysed between January 2019 and December 2021 and linked with antibiotic susceptibility testing. Bacterial isolates were subjected to whole genome sequencing (WGS).
Results: Of 142 participants, 141 (99%) were MSM and 118 (84%) living with HIV. Participants were treated with ceftriaxone (N = 79), azithromycin (N = 2), or a combination of both (N = 61). No clinical or microbiological failures were observed. From 182 positive PCR samples taken, 23 were available for detailed analysis. Based on minimal inhibitory concentrations (MICs), all isolates were susceptible to ceftriaxone, gentamicin, cefixime, cefpodoxime, ertapenem, zoliflodacin, and spectinomycin. Resistance to azithromycin, tetracyclines and ciprofloxacin was observed in 10 (43%), 23 (100%) and 11 (48%) of the cases, respectively. Analysis of WGS data revealed combinations of resistance determinants that matched with the corresponding phenotypic resistance pattern of each isolate.
Conclusion: Among the MSM diagnosed with NG mainly acquired in Switzerland, ceftriaxone MICs were low for a subset of bacterial isolates studied and no treatment failures were observed. For azithromycin, high occurrences of in vitro resistance were found. Gentamicin, cefixime, cefpodoxime, ertapenem, spectinomycin, and zoliflodacin displayed excellent in vitro activity against the 23 isolates underscoring their potential as alternative agents to ceftriaxone.
期刊介绍:
The International Journal of STD & AIDS provides a clinically oriented forum for investigating and treating sexually transmissible infections, HIV and AIDS. Publishing original research and practical papers, the journal contains in-depth review articles, short papers, case reports, audit reports, CPD papers and a lively correspondence column. This journal is a member of the Committee on Publication Ethics (COPE).