{"title":"图尔基耶松树当季针叶坏死症(CSNN)相关的多孢伞菌(Sydowia polyspora","authors":"Refika Ceyda Beram, Fatma Demiröz","doi":"10.1111/efp.12849","DOIUrl":null,"url":null,"abstract":"<p><i>Sydowia polyspora</i> is a common fungal species living on conifers, often occurring as an epiphyte or endophyte on asymptomatic plants and in seeds. The fungus may become pathogenic under abiotic or biotic stress conditions. It is often associated with current season needle necrosis and shoot dieback on conifer species, especially <i>Abies</i> spp. and <i>Pinus</i> spp. throughout Europe and North America. The conifer <i>Pinus brutia</i> is common and widely distributed in Türkiye and is frequently used in reforestation. The work described here aimed to investigate the <i>S. polyspora</i> associated with needle necrosis and shoot dieback in five different <i>Pinus brutia</i> stands located in Denizli-Türkiye, to determine the severity of the disease and to reveal the pathogenicity of this agent in <i>P. brutia</i> seedlings. For this purpose, fungal isolations from symptomatic <i>P. brutia</i> needles were carried out in five different stands. In total, 68 <i>S. polyspora</i> isolates were obtained, each from a different tree from which macroscopic-microscopic morphological characterization was made and molecular characterization was done using the internal transcribed spacer (ITS) region. BLAST analysis of the ITS sequences confirmed the identification of the isolates, with 99–100% similarity to previously reported sequences of <i>S. polyspora</i>. Disease incidence and severity were assessed in five <i>P. brutia</i> stands. The disease incidence ranged between 38% and 62% (mean = 47%) while disease severity varied among sites. Pathogenicity studies were carried out on 2-year-old <i>P. brutia</i> seedlings using obtained isolates. Infection frequency in inoculated shoots was 90%. The pathogenicity tests, followed by re-isolation of the fungus from symptomatic shoots clearly demonstrated that <i>S. polyspora</i> played a role in the development of needle necrosis and shoot dieback in <i>P. brutia</i> stands. To the best of our knowledge, this is the first report of current season needle necrosis and shoot dieback on <i>P. brutia</i> caused by <i>S. polyspora</i> in Türkiye.</p>","PeriodicalId":55153,"journal":{"name":"Forest Pathology","volume":"54 1","pages":""},"PeriodicalIF":1.3000,"publicationDate":"2024-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Sydowia polyspora associated with current season needle necrosis (CSNN) on Pinus brutia Ten. in Türkiye\",\"authors\":\"Refika Ceyda Beram, Fatma Demiröz\",\"doi\":\"10.1111/efp.12849\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><i>Sydowia polyspora</i> is a common fungal species living on conifers, often occurring as an epiphyte or endophyte on asymptomatic plants and in seeds. The fungus may become pathogenic under abiotic or biotic stress conditions. It is often associated with current season needle necrosis and shoot dieback on conifer species, especially <i>Abies</i> spp. and <i>Pinus</i> spp. throughout Europe and North America. The conifer <i>Pinus brutia</i> is common and widely distributed in Türkiye and is frequently used in reforestation. The work described here aimed to investigate the <i>S. polyspora</i> associated with needle necrosis and shoot dieback in five different <i>Pinus brutia</i> stands located in Denizli-Türkiye, to determine the severity of the disease and to reveal the pathogenicity of this agent in <i>P. brutia</i> seedlings. For this purpose, fungal isolations from symptomatic <i>P. brutia</i> needles were carried out in five different stands. In total, 68 <i>S. polyspora</i> isolates were obtained, each from a different tree from which macroscopic-microscopic morphological characterization was made and molecular characterization was done using the internal transcribed spacer (ITS) region. BLAST analysis of the ITS sequences confirmed the identification of the isolates, with 99–100% similarity to previously reported sequences of <i>S. polyspora</i>. Disease incidence and severity were assessed in five <i>P. brutia</i> stands. The disease incidence ranged between 38% and 62% (mean = 47%) while disease severity varied among sites. Pathogenicity studies were carried out on 2-year-old <i>P. brutia</i> seedlings using obtained isolates. Infection frequency in inoculated shoots was 90%. The pathogenicity tests, followed by re-isolation of the fungus from symptomatic shoots clearly demonstrated that <i>S. polyspora</i> played a role in the development of needle necrosis and shoot dieback in <i>P. brutia</i> stands. 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引用次数: 0
摘要
Sydowia polyspora 是一种生活在针叶树上的常见真菌,通常作为附生植物或内生菌出现在无症状植物和种子中。在非生物或生物胁迫条件下,这种真菌可能成为致病菌。在整个欧洲和北美,它通常与针叶树种的当季针叶坏死和嫩枝枯萎有关,特别是松柏属和松属。针叶树 Pinus brutia 在土耳其很常见,分布广泛,经常用于重新造林。本文描述的工作旨在调查位于土耳其代尼兹利的五个不同的欧洲赤松林中与针叶坏死和嫩枝枯死相关的多孢子囊菌,确定该疾病的严重程度,并揭示该病原体在欧洲赤松幼苗中的致病性。为此,研究人员在五个不同的林区从有症状的欧洲赤松针叶中进行了真菌分离。总共获得了 68 个 S. polyspora 分离物,每个分离物来自不同的树木,对分离物进行了宏观-微观形态学鉴定,并使用内部转录间隔区(ITS)进行了分子鉴定。ITS 序列的 BLAST 分析证实了分离物的鉴定结果,它们与之前报道的多孢子虫序列的相似度为 99%-100%。评估了五个 P. brutia 林分的病害发生率和严重程度。病害发生率在 38% 到 62% 之间(平均值 = 47%),病害严重程度因地点而异。利用获得的分离物对 2 年生的 P. brutia幼苗进行了致病性研究。接种嫩枝的感染率为 90%。致病性试验以及从有症状的嫩枝中重新分离真菌的结果都清楚地表明,多孢子菌在 P. brutia 林木针叶坏死和嫩枝枯死的发展过程中发挥了作用。据我们所知,这是土耳其首次报道多孢子菌在 P. brutia 上引起的当季针叶坏死和嫩枝枯死。
Sydowia polyspora associated with current season needle necrosis (CSNN) on Pinus brutia Ten. in Türkiye
Sydowia polyspora is a common fungal species living on conifers, often occurring as an epiphyte or endophyte on asymptomatic plants and in seeds. The fungus may become pathogenic under abiotic or biotic stress conditions. It is often associated with current season needle necrosis and shoot dieback on conifer species, especially Abies spp. and Pinus spp. throughout Europe and North America. The conifer Pinus brutia is common and widely distributed in Türkiye and is frequently used in reforestation. The work described here aimed to investigate the S. polyspora associated with needle necrosis and shoot dieback in five different Pinus brutia stands located in Denizli-Türkiye, to determine the severity of the disease and to reveal the pathogenicity of this agent in P. brutia seedlings. For this purpose, fungal isolations from symptomatic P. brutia needles were carried out in five different stands. In total, 68 S. polyspora isolates were obtained, each from a different tree from which macroscopic-microscopic morphological characterization was made and molecular characterization was done using the internal transcribed spacer (ITS) region. BLAST analysis of the ITS sequences confirmed the identification of the isolates, with 99–100% similarity to previously reported sequences of S. polyspora. Disease incidence and severity were assessed in five P. brutia stands. The disease incidence ranged between 38% and 62% (mean = 47%) while disease severity varied among sites. Pathogenicity studies were carried out on 2-year-old P. brutia seedlings using obtained isolates. Infection frequency in inoculated shoots was 90%. The pathogenicity tests, followed by re-isolation of the fungus from symptomatic shoots clearly demonstrated that S. polyspora played a role in the development of needle necrosis and shoot dieback in P. brutia stands. To the best of our knowledge, this is the first report of current season needle necrosis and shoot dieback on P. brutia caused by S. polyspora in Türkiye.
期刊介绍:
This peer reviewed, highly specialized journal covers forest pathological problems occurring in any part of the world. Research and review articles, short communications and book reviews are addressed to the professional, working with forest tree diseases caused by fungi, bacteria, nematodes, viruses, and phytoplasms; their biology, morphology, and pathology; disorders arising from genetic anomalies and physical or chemical factors in the environment. Articles are published in English.
Fields of interest: Forest pathology, effects of air pollution and adverse environmental conditions on trees and forest ecosystems.