{"title":"重复接种瘤胃液可加速瘤胃细菌过渡,但对产后荷斯坦奶牛的生产性能没有益处","authors":"Fanlin Kong, Feiran Wang, Yijia Zhang, Shuo Wang, Wei Wang, Shengli Li","doi":"10.1186/s40104-023-00963-9","DOIUrl":null,"url":null,"abstract":"The dairy cow’s postpartum period is characterized by dramatic physiological changes, therefore imposing severe challenges on the animal for maintaining health and milk output. The dynamics of the ruminal microbiota are also tremendous and may play a crucial role in lactation launch. We aim to investigate the potential benefits of early microbial intervention by fresh rumen microbiota transplantation (RMT) and sterile RMT in postpartum dairy cows. Twelve fistulated peak-lactation dairy cows were selected to be the donors for rumen fluid collection. Thirty postpartum cows were divided into 3 groups as the transplantation receptors respectively receiving 10 L fresh rumen fluid (FR), 10 L sterile rumen fluid (SR), or 10 L saline (CON) during 3 d after calving. Production performance, plasma indices, plasma lipidome, ruminal microbiome, and liver transcriptome were recorded. After fresh and sterile RMT, we found that the molar proportion of propionic acid was increased on d 7 in the FR and SR groups and the bacterial composition was also significantly changed when compared with the CON group. A similarity analysis showed that the similarities between the CON group and FR or SR group on d 7 were 48.40% or 47.85%, whereas the similarities between microbiota on d 7 and 21 in the FR and SR groups were 68.34% or 66.85%. Dry matter intake and feed efficiency were not affected by treatments. Plasma β-hydroxybutyrate concentration in the FR group was decreased and significantly different lipids mainly included phosphatidylcholine and lysophosphatidylcholine containing polyunsaturated fatty acids. Hepatic transcriptomics analysis indicated acute-phase response pathways were upregulated in the SR group. Our study suggests that RMT can shorten the transition process of the ruminal microbiota of postpartum dairy cows with no benefit on dry matter intake or feed efficiency. Inoculation with rumen fluid may not be a useful approach to promote the recovery of postpartum dairy cows. ","PeriodicalId":14928,"journal":{"name":"Journal of Animal Science and Biotechnology","volume":"39 1","pages":""},"PeriodicalIF":7.0000,"publicationDate":"2024-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Repeated inoculation with rumen fluid accelerates the rumen bacterial transition with no benefit on production performance in postpartum Holstein dairy cows\",\"authors\":\"Fanlin Kong, Feiran Wang, Yijia Zhang, Shuo Wang, Wei Wang, Shengli Li\",\"doi\":\"10.1186/s40104-023-00963-9\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The dairy cow’s postpartum period is characterized by dramatic physiological changes, therefore imposing severe challenges on the animal for maintaining health and milk output. The dynamics of the ruminal microbiota are also tremendous and may play a crucial role in lactation launch. We aim to investigate the potential benefits of early microbial intervention by fresh rumen microbiota transplantation (RMT) and sterile RMT in postpartum dairy cows. Twelve fistulated peak-lactation dairy cows were selected to be the donors for rumen fluid collection. Thirty postpartum cows were divided into 3 groups as the transplantation receptors respectively receiving 10 L fresh rumen fluid (FR), 10 L sterile rumen fluid (SR), or 10 L saline (CON) during 3 d after calving. Production performance, plasma indices, plasma lipidome, ruminal microbiome, and liver transcriptome were recorded. After fresh and sterile RMT, we found that the molar proportion of propionic acid was increased on d 7 in the FR and SR groups and the bacterial composition was also significantly changed when compared with the CON group. A similarity analysis showed that the similarities between the CON group and FR or SR group on d 7 were 48.40% or 47.85%, whereas the similarities between microbiota on d 7 and 21 in the FR and SR groups were 68.34% or 66.85%. Dry matter intake and feed efficiency were not affected by treatments. Plasma β-hydroxybutyrate concentration in the FR group was decreased and significantly different lipids mainly included phosphatidylcholine and lysophosphatidylcholine containing polyunsaturated fatty acids. Hepatic transcriptomics analysis indicated acute-phase response pathways were upregulated in the SR group. Our study suggests that RMT can shorten the transition process of the ruminal microbiota of postpartum dairy cows with no benefit on dry matter intake or feed efficiency. 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引用次数: 0
摘要
奶牛产后的生理变化非常剧烈,因此对奶牛保持健康和产奶量提出了严峻的挑战。瘤胃微生物群的动态变化也是巨大的,并可能在泌乳启动过程中发挥关键作用。我们旨在研究通过新鲜瘤胃微生物群移植(RMT)和无菌瘤胃微生物群移植对产后奶牛进行早期微生物干预的潜在益处。我们选择了 12 头泌乳高峰期奶牛作为瘤胃液采集的供体。将 30 头产后奶牛分为 3 组,作为移植受体,分别在产犊后 3 d 内接受 10 L 新鲜瘤胃液(FR)、10 L 无菌瘤胃液(SR)或 10 L 生理盐水(CON)。记录生产性能、血浆指数、血浆脂质组、瘤胃微生物组和肝脏转录组。经过新鲜和无菌瘤胃饲养后,我们发现,与CON组相比,FR组和SR组在第7天的瘤胃酸摩尔比例有所增加,细菌组成也发生了显著变化。相似性分析表明,CON 组与 FR 组或 SR 组在第 7 天的相似性分别为 48.40% 和 47.85%,而 FR 组和 SR 组在第 7 天和第 21 天的微生物群相似性分别为 68.34% 和 66.85%。干物质摄入量和饲料效率不受处理的影响。FR组血浆中β-羟基丁酸浓度降低,脂质显著不同,主要包括磷脂酰胆碱和溶血磷脂酰胆碱(含多不饱和脂肪酸)。肝脏转录组学分析表明,SR 组急性期反应通路上调。我们的研究表明,RMT 可缩短产后奶牛瘤胃微生物群的过渡过程,但对干物质摄入量或饲料效率无益。接种瘤胃液可能不是促进产后奶牛恢复的有效方法。
Repeated inoculation with rumen fluid accelerates the rumen bacterial transition with no benefit on production performance in postpartum Holstein dairy cows
The dairy cow’s postpartum period is characterized by dramatic physiological changes, therefore imposing severe challenges on the animal for maintaining health and milk output. The dynamics of the ruminal microbiota are also tremendous and may play a crucial role in lactation launch. We aim to investigate the potential benefits of early microbial intervention by fresh rumen microbiota transplantation (RMT) and sterile RMT in postpartum dairy cows. Twelve fistulated peak-lactation dairy cows were selected to be the donors for rumen fluid collection. Thirty postpartum cows were divided into 3 groups as the transplantation receptors respectively receiving 10 L fresh rumen fluid (FR), 10 L sterile rumen fluid (SR), or 10 L saline (CON) during 3 d after calving. Production performance, plasma indices, plasma lipidome, ruminal microbiome, and liver transcriptome were recorded. After fresh and sterile RMT, we found that the molar proportion of propionic acid was increased on d 7 in the FR and SR groups and the bacterial composition was also significantly changed when compared with the CON group. A similarity analysis showed that the similarities between the CON group and FR or SR group on d 7 were 48.40% or 47.85%, whereas the similarities between microbiota on d 7 and 21 in the FR and SR groups were 68.34% or 66.85%. Dry matter intake and feed efficiency were not affected by treatments. Plasma β-hydroxybutyrate concentration in the FR group was decreased and significantly different lipids mainly included phosphatidylcholine and lysophosphatidylcholine containing polyunsaturated fatty acids. Hepatic transcriptomics analysis indicated acute-phase response pathways were upregulated in the SR group. Our study suggests that RMT can shorten the transition process of the ruminal microbiota of postpartum dairy cows with no benefit on dry matter intake or feed efficiency. Inoculation with rumen fluid may not be a useful approach to promote the recovery of postpartum dairy cows.
期刊介绍:
Journal of Animal Science and Biotechnology is an open access, peer-reviewed journal that encompasses all aspects of animal science and biotechnology. That includes domestic animal production, animal genetics and breeding, animal reproduction and physiology, animal nutrition and biochemistry, feed processing technology and bioevaluation, animal biotechnology, and meat science.