Pub Date : 2024-11-14DOI: 10.1186/s40104-024-01108-2
Bin Wang, Xiaodan Zhang, Yongfa Liu, Mingkun Gao, Mi Wang, Yuan Wang, Xinzhi Wang, Yuming Guo
Research on low-protein-level diets has indicated that even though the profiles of essential amino acids (EAAs) follow the recommendation for a normal-protein-level diet, broilers fed low-protein diets failed to achieve productive performance compared to those fed normal diets. Therefore, it is imperative to reassess the optimum profile of EAAs in low-protein diets and establish a new ideal pattern for amino acid balance. Furthermore, identifying novel sensitive biomarkers for assessing amino acid balance will greatly facilitate the development of amino acid nutrition and application technology. In this study, 12 dietary treatments [Con(+), Con(-), L&A(-), L&A(+), M&C(-), M&C(+), BCAA (-), BCAA(+), Thr(-), Thr(+), Trp(-) and Trp(+)] were established by combining different EAAs including lysine and arginine, methionine and cysteine, branched-chain amino acid (BCAA), threonine, and tryptophan to observe the growth and development of the broiler chickens fed with low-protein-level diets. Based on the biochemical parameters and untargeted metabolomic analysis of animals subjected to different treatments, biomarkers associated with optimal and suboptimal amino acid balance were identified. Growth performance, carcass characteristics, hepatic enzyme activity, serum biochemical parameters, and breast muscle mRNA expression differed significantly between male and female broilers under different dietary amino acid patterns. Male broilers exhibited higher sensitivity to the adjustment of amino acid patterns than female broilers. For the low-protein diet, the dietary concentrations of lysine, arginine, and tryptophan, but not of methionine, cystine, or threonine, needed to be increased. Therefore, further research on individual BCAA is required. For untargeted metabolomic analysis, Con(+) was selected as a normal diet (NP) while Con(-) represented a low-protein diet (LP). L&A(+) denotes a low-protein amino acid balanced diet (LPAB) and Thr(+) represents a low-protein amino acid imbalance diet (LPAI). The metabolites oxypurinol, pantothenic acid, and D-octopine in birds were significantly influenced by different dietary amino acid patterns. Adjusting the amino acid profile of low-protein diets is required to achieve normal growth performance in broiler chickens fed normal-protein diets. Oxypurinol, pantothenic acid, and D-octopine have been identified as potentially sensitive biomarkers for assessing amino acid balance.
{"title":"Assessment of the dietary amino acid profiles and the relative biomarkers for amino acid balance in the low-protein diets for broiler chickens","authors":"Bin Wang, Xiaodan Zhang, Yongfa Liu, Mingkun Gao, Mi Wang, Yuan Wang, Xinzhi Wang, Yuming Guo","doi":"10.1186/s40104-024-01108-2","DOIUrl":"https://doi.org/10.1186/s40104-024-01108-2","url":null,"abstract":"Research on low-protein-level diets has indicated that even though the profiles of essential amino acids (EAAs) follow the recommendation for a normal-protein-level diet, broilers fed low-protein diets failed to achieve productive performance compared to those fed normal diets. Therefore, it is imperative to reassess the optimum profile of EAAs in low-protein diets and establish a new ideal pattern for amino acid balance. Furthermore, identifying novel sensitive biomarkers for assessing amino acid balance will greatly facilitate the development of amino acid nutrition and application technology. In this study, 12 dietary treatments [Con(+), Con(-), L&A(-), L&A(+), M&C(-), M&C(+), BCAA (-), BCAA(+), Thr(-), Thr(+), Trp(-) and Trp(+)] were established by combining different EAAs including lysine and arginine, methionine and cysteine, branched-chain amino acid (BCAA), threonine, and tryptophan to observe the growth and development of the broiler chickens fed with low-protein-level diets. Based on the biochemical parameters and untargeted metabolomic analysis of animals subjected to different treatments, biomarkers associated with optimal and suboptimal amino acid balance were identified. Growth performance, carcass characteristics, hepatic enzyme activity, serum biochemical parameters, and breast muscle mRNA expression differed significantly between male and female broilers under different dietary amino acid patterns. Male broilers exhibited higher sensitivity to the adjustment of amino acid patterns than female broilers. For the low-protein diet, the dietary concentrations of lysine, arginine, and tryptophan, but not of methionine, cystine, or threonine, needed to be increased. Therefore, further research on individual BCAA is required. For untargeted metabolomic analysis, Con(+) was selected as a normal diet (NP) while Con(-) represented a low-protein diet (LP). L&A(+) denotes a low-protein amino acid balanced diet (LPAB) and Thr(+) represents a low-protein amino acid imbalance diet (LPAI). The metabolites oxypurinol, pantothenic acid, and D-octopine in birds were significantly influenced by different dietary amino acid patterns. Adjusting the amino acid profile of low-protein diets is required to achieve normal growth performance in broiler chickens fed normal-protein diets. Oxypurinol, pantothenic acid, and D-octopine have been identified as potentially sensitive biomarkers for assessing amino acid balance.","PeriodicalId":14928,"journal":{"name":"Journal of Animal Science and Biotechnology","volume":"35 1","pages":""},"PeriodicalIF":7.0,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142610155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The antibacterial and immunomodulatory activities of bacteriocins make them attractive targets for development as anti-infective drugs. Although the importance of the enteric nervous system (ENS) in the struggle against infections of the intestine has been demonstrated, whether it is involved in bacteriocins anti-infective mechanisms is poorly defined. Here, we demonstrated that the bacteriocin Microcin J25 (J25) significantly alleviated diarrhea and intestinal inflammation in piglets caused by enterotoxigenic Escherichia coli (ETEC) infection. Mechanistically, macrophage levels were significantly downregulated after J25 treatment, and this was replicated in a mouse model. Omics analysis and validation screening revealed that J25 treatment induced significant changes in the dopaminergic neuron pathway, but little change in microbial structure. The alleviation of inflammation may occur by down-regulating dopamine receptor (DR) D1 and the downstream DAG-PKC pathway, thus inhibiting arachidonic acid decomposition, and the inhibition of macrophages may occur through the up-regulation of DRD5 and the downstream cAMP-PKA pathway, thus inhibiting NF-κB. Our studies’ findings provide insight into the changes and possible roles of the ENS in J25 treatment of ETEC infection, providing a more sophisticated foundational understanding for developing the application potential of J25.
{"title":"Bacteriocin Microcin J25’s antibacterial infection effects and novel non-microbial regulatory mechanisms: differential regulation of dopaminergic receptors","authors":"Lijun Shang, Fengjuan Yang, Qingyun Chen, Ziqi Dai, Guangxin Yang, Xiangfang Zeng, Shiyan Qiao, Haitao Yu","doi":"10.1186/s40104-024-01115-3","DOIUrl":"https://doi.org/10.1186/s40104-024-01115-3","url":null,"abstract":"The antibacterial and immunomodulatory activities of bacteriocins make them attractive targets for development as anti-infective drugs. Although the importance of the enteric nervous system (ENS) in the struggle against infections of the intestine has been demonstrated, whether it is involved in bacteriocins anti-infective mechanisms is poorly defined. Here, we demonstrated that the bacteriocin Microcin J25 (J25) significantly alleviated diarrhea and intestinal inflammation in piglets caused by enterotoxigenic Escherichia coli (ETEC) infection. Mechanistically, macrophage levels were significantly downregulated after J25 treatment, and this was replicated in a mouse model. Omics analysis and validation screening revealed that J25 treatment induced significant changes in the dopaminergic neuron pathway, but little change in microbial structure. The alleviation of inflammation may occur by down-regulating dopamine receptor (DR) D1 and the downstream DAG-PKC pathway, thus inhibiting arachidonic acid decomposition, and the inhibition of macrophages may occur through the up-regulation of DRD5 and the downstream cAMP-PKA pathway, thus inhibiting NF-κB. Our studies’ findings provide insight into the changes and possible roles of the ENS in J25 treatment of ETEC infection, providing a more sophisticated foundational understanding for developing the application potential of J25.","PeriodicalId":14928,"journal":{"name":"Journal of Animal Science and Biotechnology","volume":"69 1","pages":""},"PeriodicalIF":7.0,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142601115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-12DOI: 10.1186/s40104-024-01113-5
Dan Hu, Xiaoran Yang, Ming Qin, Li’an Pan, Haiyan Fang, Pengnan Chen, Yingdong Ni
Salmonella Typhimurium (S. Typhimurium) is a common pathogenic microorganism and poses a threat to the efficiency of poultry farms. As signaling molecules regulating the interaction between the host and gut microbiota, bile acids (BAs) play a protective role in maintaining gut homeostasis. However, the antibacterial effect of BAs on Salmonella infection in broilers has remained unexplored. Therefore, the aim of this study was to investigate the potential role of feeding BAs in protecting against S. Typhimurium infection in broilers. A total of 144 1-day-old Arbor Acres male broilers were randomly assigned to 4 groups, including non-challenged birds fed a basal diet (CON), S. Typhimurium-challenged birds (ST), S. Typhimurium-challenged birds treated with 0.15 g/kg antibiotic after infection (ST-ANT), and S. Typhimurium-challenged birds fed a basal diet supplemented with 350 mg/kg of BAs (ST-BA). BAs supplementation ameliorated weight loss induced by S. Typhimurium infection and reduced the colonization of Salmonella in the liver and small intestine in broilers (P < 0.05). Compared to the ST group, broilers in ST-BA group had a higher ileal mucosal thickness and villus height, and BAs also ameliorated the increase of diamine oxidase (DAO) level in serum (P < 0.05). It was observed that the mucus layer thickness and the number of villous and cryptic goblet cells (GCs) were increased in the ST-BA group, consistent with the upregulation of MUC2 gene expression in the ileal mucosa (P < 0.05). Moreover, the mRNA expressions of Toll-like receptor 5 (TLR5), Toll-like receptor 4 (TLR4), and interleukin 1 beta (IL1b) were downregulated in the ileum by BAs treatment (P < 0.05). 16S rDNA sequencing analysis revealed that, compared to ST group, BAs ameliorated the decreases in Bacteroidota, Bacteroidaceae and Bacteroides abundances, which were negatively correlated with serum DAO activity, and the increases in Campylobacterota, Campylobacteraceae and Campylobacter abundances, which were negatively correlated with body weight but positively correlated with serum D-lactic acid (D-LA) levels (P < 0.05). Dietary BAs supplementation strengthens the intestinal mucosal barrier and reverses dysbiosis of gut microbiota, which eventually relieves the damage to the intestinal barrier and weight loss induced by S. Typhimurium infection in broilers.
鼠伤寒沙门氏菌（S. Typhimurium）是一种常见的病原微生物，对家禽养殖场的效率构成威胁。作为调节宿主与肠道微生物群之间相互作用的信号分子，胆汁酸（BAs）在维持肠道平衡方面发挥着保护作用。然而，胆汁酸对肉鸡沙门氏菌感染的抗菌作用仍有待探索。因此，本研究旨在探讨饲喂 BAs 在防止肉鸡感染鼠伤寒沙门氏菌方面的潜在作用。研究人员将144只1日龄的Arbor Acres雄性肉鸡随机分为4组，包括饲喂基础日粮（CON）的未受感染鸡、受鼠伤寒杆菌感染的鸡（ST）、受鼠伤寒杆菌感染后使用0.15克/千克抗生素治疗的鸡（ST-ANT）以及饲喂添加了350毫克/千克BAs的基础日粮（ST-BA）的受鼠伤寒杆菌感染的鸡（ST-BA）。补充 BAs 可改善伤寒杆菌感染引起的体重下降，并减少沙门氏菌在肉鸡肝脏和小肠中的定植（P < 0.05）。与 ST 组相比，ST-BA 组肉鸡的回肠粘膜厚度和绒毛高度更高，而且 BA 还能改善血清中二胺氧化酶（DAO）水平的升高（P < 0.05）。观察发现，ST-BA 组黏液层厚度、绒毛和隐性鹅口疮细胞（GCs）数量增加，与回肠黏膜 MUC2 基因表达上调一致（P < 0.05）。此外，Toll 样受体 5（TLR5）、Toll 样受体 4（TLR4）和白细胞介素 1 beta（IL1b）的 mRNA 表达在 BAs 处理后的回肠中下调（P < 0.05）。16S rDNA 测序分析表明，与 ST 组相比，BAs 可改善类杆菌属、类杆菌科和类杆菌丰度的下降（与血清 DAO 活性呈负相关），以及弯曲杆菌属、弯曲杆菌科和弯曲杆菌丰度的增加（与体重呈负相关，但与血清 D-乳酸（D-LA）水平呈正相关）（P < 0.05）。膳食中补充 BAs 可增强肠道黏膜屏障，逆转肠道微生物区系失调，最终缓解伤寒杆菌感染对肉鸡肠道屏障的破坏和体重下降。
{"title":"Dietary bile acids supplementation protects against Salmonella Typhimurium infection via improving intestinal mucosal barrier and gut microbiota composition in broilers","authors":"Dan Hu, Xiaoran Yang, Ming Qin, Li’an Pan, Haiyan Fang, Pengnan Chen, Yingdong Ni","doi":"10.1186/s40104-024-01113-5","DOIUrl":"https://doi.org/10.1186/s40104-024-01113-5","url":null,"abstract":"Salmonella Typhimurium (S. Typhimurium) is a common pathogenic microorganism and poses a threat to the efficiency of poultry farms. As signaling molecules regulating the interaction between the host and gut microbiota, bile acids (BAs) play a protective role in maintaining gut homeostasis. However, the antibacterial effect of BAs on Salmonella infection in broilers has remained unexplored. Therefore, the aim of this study was to investigate the potential role of feeding BAs in protecting against S. Typhimurium infection in broilers. A total of 144 1-day-old Arbor Acres male broilers were randomly assigned to 4 groups, including non-challenged birds fed a basal diet (CON), S. Typhimurium-challenged birds (ST), S. Typhimurium-challenged birds treated with 0.15 g/kg antibiotic after infection (ST-ANT), and S. Typhimurium-challenged birds fed a basal diet supplemented with 350 mg/kg of BAs (ST-BA). BAs supplementation ameliorated weight loss induced by S. Typhimurium infection and reduced the colonization of Salmonella in the liver and small intestine in broilers (P < 0.05). Compared to the ST group, broilers in ST-BA group had a higher ileal mucosal thickness and villus height, and BAs also ameliorated the increase of diamine oxidase (DAO) level in serum (P < 0.05). It was observed that the mucus layer thickness and the number of villous and cryptic goblet cells (GCs) were increased in the ST-BA group, consistent with the upregulation of MUC2 gene expression in the ileal mucosa (P < 0.05). Moreover, the mRNA expressions of Toll-like receptor 5 (TLR5), Toll-like receptor 4 (TLR4), and interleukin 1 beta (IL1b) were downregulated in the ileum by BAs treatment (P < 0.05). 16S rDNA sequencing analysis revealed that, compared to ST group, BAs ameliorated the decreases in Bacteroidota, Bacteroidaceae and Bacteroides abundances, which were negatively correlated with serum DAO activity, and the increases in Campylobacterota, Campylobacteraceae and Campylobacter abundances, which were negatively correlated with body weight but positively correlated with serum D-lactic acid (D-LA) levels (P < 0.05). Dietary BAs supplementation strengthens the intestinal mucosal barrier and reverses dysbiosis of gut microbiota, which eventually relieves the damage to the intestinal barrier and weight loss induced by S. Typhimurium infection in broilers.","PeriodicalId":14928,"journal":{"name":"Journal of Animal Science and Biotechnology","volume":"4 1","pages":""},"PeriodicalIF":7.0,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142599665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-11DOI: 10.1186/s40104-024-01123-3
Minghui Wang, Kelin Li, Hongchao Jiao, Jingpeng Zhao, Haifang Li, Yunlei Zhou, Aizhi Cao, Jianmin Wang, Xiaojuan Wang, Hai Lin
<p> <b>Correction</b><b>: </b> <b>J Animal Sci Biotechnol 15, 113 (2024)</b> </p><p><b>https://doi.org/10.1186/s40104-024-01071-y</b></p><br/><p>Following publication of the original article [1], the authors reported a typo in the author name.</p><p>The author name <b>Jianming Wang</b> should be corrected to <b>Jianmin Wang</b>.</p><p>The original article [1] has been updated.</p><ol data-track-component="outbound reference" data-track-context="references section"><li data-counter="1."><p>Wang M, Li K, Jiao H, et al. Dietary bile acids supplementation decreases hepatic fat deposition with the involvement of altered gut microbiota and liver bile acids profile in broiler chickens. J Animal Sci Biotechnol. 2024;15:113. https://doi.org/10.1186/s40104-024-01071-y.</p><p>Article CAS Google Scholar </p></li></ol><p>Download references<svg aria-hidden="true" focusable="false" height="16" role="img" width="16"><use xlink:href="#icon-eds-i-download-medium" xmlns:xlink="http://www.w3.org/1999/xlink"></use></svg></p><h3>Authors and Affiliations</h3><ol><li><p>College of Animal Science and Technology, Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Key Laboratory of Efficient Utilization of Non-Grain Feed Resources (Co-Construction By Ministry and Province), Ministry of Agriculture and Rural Affairs, Shandong Agricultural University, No. 61, Daizong Street, Taian, 271018, Shandong, P. R. China</p><p>Minghui Wang, Kelin Li, Hongchao Jiao, Jingpeng Zhao, Xiaojuan Wang & Hai Lin</p></li><li><p>College of Life Sciences, Shandong Agricultural University, No. 61, Daizong Street, Taian, 271018, Shandong, P. R. China</p><p>Haifang Li</p></li><li><p>College of Chemistry and Material Science, Shandong Agricultural University, No. 61, Daizong Street, Taian, 271018, Shandong, P. R. China</p><p>Yunlei Zhou</p></li><li><p>Shandong Longchang Animal Health Products Co., Ltd., Jinan, P. R. China</p><p>Aizhi Cao & Jianmin Wang</p></li></ol><span>Authors</span><ol><li><span>Minghui Wang</span>View author publications<p>You can also search for this author in <span>PubMed<span> </span>Google Scholar</span></p></li><li><span>Kelin Li</span>View author publications<p>You can also search for this author in <span>PubMed<span> </span>Google Scholar</span></p></li><li><span>Hongchao Jiao</span>View author publications<p>You can also search for this author in <span>PubMed<span> </span>Google Scholar</span></p></li><li><span>Jingpeng Zhao</span>View author publications<p>You can also search for this author in <span>PubMed<span> </span>Google Scholar</span></p></li><li><span>Haifang Li</span>View author publications<p>You can also search for this author in <span>PubMed<span> </span>Google Scholar</span></p></li><li><span>Yunlei Zhou</span>View author publications<p>You can also search for this author in <span>PubMed<span> </span>Google Scholar</span></p></li><li><span>Aizhi Cao</span>View author publications<p>You can also search for
更正：J Animal Sci Biotechnol 15, 113 (2024)https://doi.org/10.1186/s40104-024-01071-yFollowing 原文[1]发表时，作者报告了作者姓名中的一个错字。作者姓名Jianming Wang应更正为Jianmin Wang。原文[1]已更新。Wang M, Li K, Jiao H, et al. Dietary bile acids supplementation decreases hepatic fat deposition with the involvement of altered gut microbiota and liver bile acids profile in broiler chickens.J Animal Sci Biotechnol.2024;15:113. https://doi.org/10.1186/s40104-024-01071-y.Article CAS Google Scholar 下载参考文献作者与单位山东农业大学动物科技学院，山东省动物生物技术与疫病防控重点实验室，农业农村部非粮饲料资源高效利用重点实验室（部省共建），山东农业大学，泰安市岱宗大街61号。山东省泰安市岱宗大街 61 号 山东农业大学生命科学学院 王明辉 李克林 焦洪超 赵景鹏 王晓娟 & Hai LinCollege of Life Sciences, Shandong Agricultural University, No.李海芳 山东省泰安市岱宗大街 61 号山东农业大学化学与材料科学学院 邮编：271018 周云雷 山东龙昌动物保健品有限公司，泰安市岱岳区岱宗大街 61 号、山东龙昌动物保健品有限公司中国济南中国曹爱芝 &；王建民作者：王明辉查看作者发表的论文您也可以在PubMed Google Scholar中搜索该作者李克林查看作者发表的论文您也可以在PubMed Google Scholar中搜索该作者焦洪超查看作者发表的论文您也可以在PubMed Google Scholar中搜索该作者赵景鹏查看作者发表的论文您也可以在PubMed Google Scholar中搜索该作者李海芳查看作者发表的论文您也可以在PubMed Google Scholar中搜索该作者周云雷查看作者发表的论文您也可以在PubMed Google Scholar中搜索该作者周云雷查看作者发表的论文您也可以在PubMed Google Scholar中搜索该作者周云雷查看作者发表的论文发表文章您也可以在PubMed Google Scholar中搜索该作者曹爱芝查看发表文章您也可以在PubMed Google Scholar中搜索该作者王建民查看发表文章您也可以在PubMed Google Scholar中搜索该作者王晓娟查看发表文章您也可以在PubMed Google Scholar中搜索该作者林海查看发表文章您也可以在PubMed Google Scholar中搜索该作者通讯作者：王晓娟或林海。开放存取 本文采用知识共享署名 4.0 国际许可协议进行许可，该协议允许以任何媒介或格式使用、共享、改编、分发和复制本文，但需注明原作者和出处，提供知识共享许可协议的链接，并说明是否进行了修改。本文中的图片或其他第三方材料均包含在文章的知识共享许可协议中，除非在材料的署名栏中另有说明。如果材料未包含在文章的知识共享许可协议中，且您打算使用的材料不符合法律规定或超出许可使用范围，则您需要直接从版权所有者处获得许可。要查看该许可的副本，请访问 http://creativecommons.org/licenses/by/4.0/。除非在数据的信用行中另有说明，否则知识共享公共领域专用免责声明（http://creativecommons.org/publicdomain/zero/1.0/）适用于本文提供的数据。转载和许可引用本文Wang, M., Li, K., Jiao, H. et al. Correction：补充胆汁酸可减少肉鸡肝脏脂肪沉积，肠道微生物群和肝脏胆汁酸谱改变参与其中J Animal Sci Biotechnol 15, 154 (2024). https://doi.org/10.1186/s40104-024-01123-3Download citationPublished: 11 November 2024DOI: https://doi.org/10.1186/s40104-024-01123-3Share this articleAnyone you share the following link with will be able to read this content:Get shareable linkSorry, a shareable link is not currently available for this article.Copy to clipboard Provided by the Springer Nature SharedIt content-sharing initiative
{"title":"Correction: Dietary bile acids supplementation decreases hepatic fat deposition with the involvement of altered gut microbiota and liver bile acids profile in broiler chickens","authors":"Minghui Wang, Kelin Li, Hongchao Jiao, Jingpeng Zhao, Haifang Li, Yunlei Zhou, Aizhi Cao, Jianmin Wang, Xiaojuan Wang, Hai Lin","doi":"10.1186/s40104-024-01123-3","DOIUrl":"https://doi.org/10.1186/s40104-024-01123-3","url":null,"abstract":"<p>\u0000<b>Correction</b><b>: </b>\u0000<b>J Animal Sci Biotechnol 15, 113 (2024)</b>\u0000</p><p><b>https://doi.org/10.1186/s40104-024-01071-y</b></p><br/><p>Following publication of the original article [1], the authors reported a typo in the author name.</p><p>The author name <b>Jianming Wang</b> should be corrected to <b>Jianmin Wang</b>.</p><p>The original article [1] has been updated.</p><ol data-track-component=\"outbound reference\" data-track-context=\"references section\"><li data-counter=\"1.\"><p>Wang M, Li K, Jiao H, et al. Dietary bile acids supplementation decreases hepatic fat deposition with the involvement of altered gut microbiota and liver bile acids profile in broiler chickens. J Animal Sci Biotechnol. 2024;15:113. https://doi.org/10.1186/s40104-024-01071-y.</p><p>Article CAS Google Scholar </p></li></ol><p>Download references<svg aria-hidden=\"true\" focusable=\"false\" height=\"16\" role=\"img\" width=\"16\"><use xlink:href=\"#icon-eds-i-download-medium\" xmlns:xlink=\"http://www.w3.org/1999/xlink\"></use></svg></p><h3>Authors and Affiliations</h3><ol><li><p>College of Animal Science and Technology, Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Key Laboratory of Efficient Utilization of Non-Grain Feed Resources (Co-Construction By Ministry and Province), Ministry of Agriculture and Rural Affairs, Shandong Agricultural University, No. 61, Daizong Street, Taian, 271018, Shandong, P. R. China</p><p>Minghui Wang, Kelin Li, Hongchao Jiao, Jingpeng Zhao, Xiaojuan Wang & Hai Lin</p></li><li><p>College of Life Sciences, Shandong Agricultural University, No. 61, Daizong Street, Taian, 271018, Shandong, P. R. China</p><p>Haifang Li</p></li><li><p>College of Chemistry and Material Science, Shandong Agricultural University, No. 61, Daizong Street, Taian, 271018, Shandong, P. R. China</p><p>Yunlei Zhou</p></li><li><p>Shandong Longchang Animal Health Products Co., Ltd., Jinan, P. R. China</p><p>Aizhi Cao & Jianmin Wang</p></li></ol><span>Authors</span><ol><li><span>Minghui Wang</span>View author publications<p>You can also search for this author in <span>PubMed<span> </span>Google Scholar</span></p></li><li><span>Kelin Li</span>View author publications<p>You can also search for this author in <span>PubMed<span> </span>Google Scholar</span></p></li><li><span>Hongchao Jiao</span>View author publications<p>You can also search for this author in <span>PubMed<span> </span>Google Scholar</span></p></li><li><span>Jingpeng Zhao</span>View author publications<p>You can also search for this author in <span>PubMed<span> </span>Google Scholar</span></p></li><li><span>Haifang Li</span>View author publications<p>You can also search for this author in <span>PubMed<span> </span>Google Scholar</span></p></li><li><span>Yunlei Zhou</span>View author publications<p>You can also search for this author in <span>PubMed<span> </span>Google Scholar</span></p></li><li><span>Aizhi Cao</span>View author publications<p>You can also search for ","PeriodicalId":14928,"journal":{"name":"Journal of Animal Science and Biotechnology","volume":"69 1","pages":""},"PeriodicalIF":7.0,"publicationDate":"2024-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142598476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-10DOI: 10.1186/s40104-024-01112-6
Gaiping Wen, Klaus Eder, Robert Ringseis
The use of conventional two-dimensional (2D) culture of the porcine intestinal epithelial cell (IEC) line IPEC-J2 in animal nutrition research has the disadvantage that IEC function is studied under unphysiological conditions, which limits the ability of transferring knowledge to the in vivo-situation. Thus, the aim of the present study was to establish a more convincing and meaningful three-dimensional (3D) culture of IPEC-J2 cells, which allows to study cell function in a more tissue-like environment, and to compare the effect of the endoplasmic reticulum (ER) stress inducer tunicamycin (TM) on ER stress indicators and the expression of tight junction proteins (TJP), inflammatory and apoptosis-related genes and the modulatory role of 1,25-dihydroxy-vitamin D3 (1,25D3) on these parameters in 2D and 3D cultures of IPEC-J2 cells. A published protocol for 3D culture of Caco-2 cells was successfully adopted to IPEC-J2 cells as evident from fully differentiated 3D IPEC-J2 spheroids showing the characteristic spherical architecture with a single layer of IPEC-J2 cells surrounding a central lumen. Treatment of 2D IPEC-J2 cells and 3D IPEC-J2 spheroids with TM for 24 h markedly increased mRNA and/or protein levels of the ER stress target genes, heat shock protein family A (Hsp70) member 5 (HSPA5) and DNA damage inducible transcript 3 (DDIT3), whereas co-treatment with TM and 1,25D3 did not mitigate TM-induced ER stress in IPEC-J2 cells in the 2D and the 3D cell culture. In contrast, TM-induced expression of pro-inflammatory [interleukin-6 (IL6), IL8] and pro-apoptotic genes [BCL2 associated X, apoptosis regulator (BAX), caspase 3 (CASP3), CASP8] and genes encoding TJP [TJP1, claudin 1 (CLDN1), CLDN3, occludin (OCLN), cadherin 1 (CDH1), junctional adhesion molecule 1 (JAM1)] was reduced by co-treatment with TM and 1,25D3 in 3D IPEC-J2 spheroids but not in the 2D cell culture. The effect of 1,25D3 in the IPEC-J2 cell culture is dependent on the culture model applied. While 1,25D3 does not inhibit TM-induced expression of genes involved in inflammation, apoptosis and TJP in conventional 2D cultures of IPEC-J2 cells, TM-induced expression of these genes is abrogated by 1,25D3 in the more meaningful 3D IPEC-J2 cell culture model.
{"title":"Comparative evaluation of the modulatory role of 1,25-dihydroxy-vitamin D3 on endoplasmic reticulum stress-induced effects in 2D and 3D cultures of the intestinal porcine epithelial cell line IPEC-J2","authors":"Gaiping Wen, Klaus Eder, Robert Ringseis","doi":"10.1186/s40104-024-01112-6","DOIUrl":"https://doi.org/10.1186/s40104-024-01112-6","url":null,"abstract":"The use of conventional two-dimensional (2D) culture of the porcine intestinal epithelial cell (IEC) line IPEC-J2 in animal nutrition research has the disadvantage that IEC function is studied under unphysiological conditions, which limits the ability of transferring knowledge to the in vivo-situation. Thus, the aim of the present study was to establish a more convincing and meaningful three-dimensional (3D) culture of IPEC-J2 cells, which allows to study cell function in a more tissue-like environment, and to compare the effect of the endoplasmic reticulum (ER) stress inducer tunicamycin (TM) on ER stress indicators and the expression of tight junction proteins (TJP), inflammatory and apoptosis-related genes and the modulatory role of 1,25-dihydroxy-vitamin D3 (1,25D3) on these parameters in 2D and 3D cultures of IPEC-J2 cells. A published protocol for 3D culture of Caco-2 cells was successfully adopted to IPEC-J2 cells as evident from fully differentiated 3D IPEC-J2 spheroids showing the characteristic spherical architecture with a single layer of IPEC-J2 cells surrounding a central lumen. Treatment of 2D IPEC-J2 cells and 3D IPEC-J2 spheroids with TM for 24 h markedly increased mRNA and/or protein levels of the ER stress target genes, heat shock protein family A (Hsp70) member 5 (HSPA5) and DNA damage inducible transcript 3 (DDIT3), whereas co-treatment with TM and 1,25D3 did not mitigate TM-induced ER stress in IPEC-J2 cells in the 2D and the 3D cell culture. In contrast, TM-induced expression of pro-inflammatory [interleukin-6 (IL6), IL8] and pro-apoptotic genes [BCL2 associated X, apoptosis regulator (BAX), caspase 3 (CASP3), CASP8] and genes encoding TJP [TJP1, claudin 1 (CLDN1), CLDN3, occludin (OCLN), cadherin 1 (CDH1), junctional adhesion molecule 1 (JAM1)] was reduced by co-treatment with TM and 1,25D3 in 3D IPEC-J2 spheroids but not in the 2D cell culture. The effect of 1,25D3 in the IPEC-J2 cell culture is dependent on the culture model applied. While 1,25D3 does not inhibit TM-induced expression of genes involved in inflammation, apoptosis and TJP in conventional 2D cultures of IPEC-J2 cells, TM-induced expression of these genes is abrogated by 1,25D3 in the more meaningful 3D IPEC-J2 cell culture model.","PeriodicalId":14928,"journal":{"name":"Journal of Animal Science and Biotechnology","volume":"196 1","pages":""},"PeriodicalIF":7.0,"publicationDate":"2024-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142597784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
During the transition period, excessive negative energy balance (NEB) lead to metabolic disorders and reduced milk yield. Rumen microbes are responsible for resolving plant material and producing volatile fatty acids (VFA), which are the primary energy source for cows. In this study, we aimed to investigate the effect of citrus peel extract (CPE) supplementation on rumen microbiota composition, energy metabolism and milk performance of peripartum dairy cows. Dairy cows were fed either a basal diet (CON group) or the same basal diet supplemented with CPE via intragastric administration (4 g/d, CPE group) for 6 weeks (3 weeks before and 3 weeks after calving; n = 15 per group). Samples of serum, milk, rumen fluid, adipose tissue, and liver were collected to assess the effects of CPE on rumen microbiota composition, rumen fermentation parameters, milk performance, and energy metabolic status of dairy cows. CPE supplementation led to an increase in milk yield, milk protein and lactose contents, and serum glucose levels, while reduced serum concentrations of non-esterified fatty acid, β-hydroxybutyric acid, insulin, aspartate aminotransferase, alanine aminotransferase, and haptoglobin during the first month of lactation. CPE supplementation also increased the content of ruminal VFA. Compared to the CON group, the abundance of Prevotellaceae, Methanobacteriaceae, Bacteroidales_RF16_group, and Selenomonadaceae was found increased, while the abundance of Oscillospiraceae, F082, Ruminococcaceae, Christensenellaceae, Muribaculaceae UCG-011, Saccharimonadaceae, Hungateiclostridiaceae, and Spirochaetaceae in the CPE group was found decreased. In adipose tissue, CPE supplementation decreased lipolysis, and inflammatory response, while increased insulin sensitivity. In the liver, CPE supplementation decreased lipid accumulation, increased insulin sensitivity, and upregulated expression of genes involved in gluconeogenesis. Our findings suggest that CPE supplementation during the peripartum period altered rumen microbiota composition and increased ruminal VFA contents, which further improved NEB and lactation performance, alleviated lipolysis and inflammatory response in adipose tissue, reduced lipid accumulation and promoted gluconeogenesis in liver. Thus, CPE might contribute to improve energy metabolism and consequently lactation performance of dairy cows during the transition period.
{"title":"Dietary supplementation with citrus peel extract in transition period improves rumen microbial composition and ameliorates energy metabolism and lactation performance of dairy cows","authors":"Lingxue Ju, Qi Shao, Zhiyuan Fang, Erminio Trevisi, Meng Chen, Yuxiang Song, Wenwen Gao, Lin Lei, Xinwei Li, Guowen Liu, Xiliang Du","doi":"10.1186/s40104-024-01110-8","DOIUrl":"https://doi.org/10.1186/s40104-024-01110-8","url":null,"abstract":"During the transition period, excessive negative energy balance (NEB) lead to metabolic disorders and reduced milk yield. Rumen microbes are responsible for resolving plant material and producing volatile fatty acids (VFA), which are the primary energy source for cows. In this study, we aimed to investigate the effect of citrus peel extract (CPE) supplementation on rumen microbiota composition, energy metabolism and milk performance of peripartum dairy cows. Dairy cows were fed either a basal diet (CON group) or the same basal diet supplemented with CPE via intragastric administration (4 g/d, CPE group) for 6 weeks (3 weeks before and 3 weeks after calving; n = 15 per group). Samples of serum, milk, rumen fluid, adipose tissue, and liver were collected to assess the effects of CPE on rumen microbiota composition, rumen fermentation parameters, milk performance, and energy metabolic status of dairy cows. CPE supplementation led to an increase in milk yield, milk protein and lactose contents, and serum glucose levels, while reduced serum concentrations of non-esterified fatty acid, β-hydroxybutyric acid, insulin, aspartate aminotransferase, alanine aminotransferase, and haptoglobin during the first month of lactation. CPE supplementation also increased the content of ruminal VFA. Compared to the CON group, the abundance of Prevotellaceae, Methanobacteriaceae, Bacteroidales_RF16_group, and Selenomonadaceae was found increased, while the abundance of Oscillospiraceae, F082, Ruminococcaceae, Christensenellaceae, Muribaculaceae UCG-011, Saccharimonadaceae, Hungateiclostridiaceae, and Spirochaetaceae in the CPE group was found decreased. In adipose tissue, CPE supplementation decreased lipolysis, and inflammatory response, while increased insulin sensitivity. In the liver, CPE supplementation decreased lipid accumulation, increased insulin sensitivity, and upregulated expression of genes involved in gluconeogenesis. Our findings suggest that CPE supplementation during the peripartum period altered rumen microbiota composition and increased ruminal VFA contents, which further improved NEB and lactation performance, alleviated lipolysis and inflammatory response in adipose tissue, reduced lipid accumulation and promoted gluconeogenesis in liver. Thus, CPE might contribute to improve energy metabolism and consequently lactation performance of dairy cows during the transition period.","PeriodicalId":14928,"journal":{"name":"Journal of Animal Science and Biotechnology","volume":"36 1","pages":""},"PeriodicalIF":7.0,"publicationDate":"2024-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142597785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Weaning stress-induced diarrhea is widely recognized as being associated with gut microbiota dysbiosis. However, it has been challenging to clarify which specific intestinal microbiota and their metabolites play a crucial role in the antidiarrhea process of weaned piglets. In this study, we first observed that piglets with diarrhea exhibited a lower average daily gain and higher diarrhea score, and elevated levels of lipopolysaccharide (LPS) and D-lactate (D-LA) compared to healthy piglets. Subsequently, we analyzed the differences in intestinal microbial composition and metabolite levels between healthy and diarrheal weaned piglets. Diarrheal piglets demonstrated intestinal microbiota dysbiosis, characterized primarily by a higher Firmicutes to Bacteroidota ratio, a deficiency of Lactobacillus amylovorus and Lactobacillus reuteri, and an increased abundance of Bacteroides sp.HF-5287 and Bacteroides thetaiotaomicron. Functional profiling of the gut microbiota based on Kyoto Encyclopedia of Genes and Genomes (KEGG) data was performed, and the results showed that tryptophan metabolism was the most significantly inhibited pathway in piglets with diarrhea. Most tryptophan metabolites were detected at lower concentrations in diarrheal piglets than in healthy piglets. Furthermore, we explored the effects of dietary indole-3-aldehyde (IAld), a key tryptophan metabolite, on intestinal development and gut barrier function in weaned piglets. Supplementation with 100 mg/kg IAld in the diet increased the small intestine index and improved intestinal barrier function by promoting intestinal stem cell (ISC) expansion in piglets. The promotion of ISC expansion by IAld was also confirmed in porcine intestinal organoids. These findings revealed that intestinal microbial tryptophan metabolite IAld alleviates impaired intestinal development by promoting ISC expansion in weaned piglets.
{"title":"The gut microbial metabolite indole-3-aldehyde alleviates impaired intestinal development by promoting intestinal stem cell expansion in weaned piglets","authors":"Jiaqi Zhang, Yahui Chen, Xin Guo, Xuan Li, Ruofan Zhang, Mengting Wang, Weiyun Zhu, Kaifan Yu","doi":"10.1186/s40104-024-01111-7","DOIUrl":"https://doi.org/10.1186/s40104-024-01111-7","url":null,"abstract":"Weaning stress-induced diarrhea is widely recognized as being associated with gut microbiota dysbiosis. However, it has been challenging to clarify which specific intestinal microbiota and their metabolites play a crucial role in the antidiarrhea process of weaned piglets. In this study, we first observed that piglets with diarrhea exhibited a lower average daily gain and higher diarrhea score, and elevated levels of lipopolysaccharide (LPS) and D-lactate (D-LA) compared to healthy piglets. Subsequently, we analyzed the differences in intestinal microbial composition and metabolite levels between healthy and diarrheal weaned piglets. Diarrheal piglets demonstrated intestinal microbiota dysbiosis, characterized primarily by a higher Firmicutes to Bacteroidota ratio, a deficiency of Lactobacillus amylovorus and Lactobacillus reuteri, and an increased abundance of Bacteroides sp.HF-5287 and Bacteroides thetaiotaomicron. Functional profiling of the gut microbiota based on Kyoto Encyclopedia of Genes and Genomes (KEGG) data was performed, and the results showed that tryptophan metabolism was the most significantly inhibited pathway in piglets with diarrhea. Most tryptophan metabolites were detected at lower concentrations in diarrheal piglets than in healthy piglets. Furthermore, we explored the effects of dietary indole-3-aldehyde (IAld), a key tryptophan metabolite, on intestinal development and gut barrier function in weaned piglets. Supplementation with 100 mg/kg IAld in the diet increased the small intestine index and improved intestinal barrier function by promoting intestinal stem cell (ISC) expansion in piglets. The promotion of ISC expansion by IAld was also confirmed in porcine intestinal organoids. These findings revealed that intestinal microbial tryptophan metabolite IAld alleviates impaired intestinal development by promoting ISC expansion in weaned piglets.","PeriodicalId":14928,"journal":{"name":"Journal of Animal Science and Biotechnology","volume":"150 1","pages":""},"PeriodicalIF":7.0,"publicationDate":"2024-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142597787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
<p><b>Correction</b><b>: </b><b>J Animal Sci Biotechnol 15, 128 (2024)</b></p><p><b>https://doi.org/10.1186/s40104-024-01088-3</b></p><br/><p>Following publication of the original article [1], the authors reported that in the original Fig. 3A, on W14, the pictures stained by DHE and DAPI fluorochrome, and the merged picture were placed out of order.</p><p>The original Fig. 3 was:</p><figure><figcaption><b data-test="figure-caption-text">Fig. 3</b></figcaption><picture><source srcset="//media.springernature.com/lw685/springer-static/image/art%3A10.1186%2Fs40104-024-01116-2/MediaObjects/40104_2024_1116_Fig1_HTML.png?as=webp" type="image/webp"/><img alt="figure 1" aria-describedby="Fig1" height="465" loading="lazy" src="//media.springernature.com/lw685/springer-static/image/art%3A10.1186%2Fs40104-024-01116-2/MediaObjects/40104_2024_1116_Fig1_HTML.png" width="685"/></picture><p>The hepatic redox parameters. <b>A</b> and <b>B</b> DHE Staining with frozen liver sections and fluorescence intensity of ROS; <b>C</b>–<b>E</b> Hepatic H<sub>2</sub>O<sub>2</sub>, O<sub>2</sub><sup>−</sup> and T-AOC content; <b>F</b>–<b>H</b> Hepatic T-SOD, CAT and GPX activity; <b>I</b> Hepatic MDA content. W0, W1, W4, W7, and W14 respectively represented 21, 22, 25, 28, and 35 days of age. Data were presented as mean ± SEM (ROS, <i>n</i> = 3; others, <i>n</i> = 6). Values with different letters differ significantly (<i>P</i> < 0.05). ROS: Reactive oxygen species; T-AOC: Total antioxidant capacity; T-SOD: Total superoxide dismutase; CAT: Catalase; GPX: Glutathione peroxidase; MDA: Malonaldehyde</p><span>Full size image</span><svg aria-hidden="true" focusable="false" height="16" role="img" width="16"><use xlink:href="#icon-eds-i-chevron-right-small" xmlns:xlink="http://www.w3.org/1999/xlink"></use></svg></figure><p>The correct Fig. 3 should be:</p><figure><figcaption><b data-test="figure-caption-text">Fig. 3</b></figcaption><picture><source srcset="//media.springernature.com/lw685/springer-static/image/art%3A10.1186%2Fs40104-024-01116-2/MediaObjects/40104_2024_1116_Fig2_HTML.png?as=webp" type="image/webp"/><img alt="figure 2" aria-describedby="Fig2" height="494" loading="lazy" src="//media.springernature.com/lw685/springer-static/image/art%3A10.1186%2Fs40104-024-01116-2/MediaObjects/40104_2024_1116_Fig2_HTML.png" width="685"/></picture><p>The hepatic redox parameters. <b>A</b> and <b>B</b> DHE Staining with frozen liver sections and fluorescence intensity of ROS; <b>C</b>–<b>E</b> Hepatic H<sub>2</sub>O<sub>2</sub>, O<sub>2</sub><sup>−</sup> and T-AOC content; <b>F</b>–<b>H</b> Hepatic T-SOD, CAT and GPX activity; <b>I</b> Hepatic MDA content. W0, W1, W4, W7, and W14 respectively represented 21, 22, 25, 28, and 35 days of age. Data were presented as mean ± SEM (ROS, <i>n</i> = 3; others, <i>n</i> = 6). Values with different letters differ significantly (<i>P</i> < 0.05). ROS: Reactive oxygen species; T-AOC: Total antioxidant capacity; T-SOD: Total superoxide dismutase;
更正：J Animal Sci Biotechnol 15, 128 (2024)https://doi.org/10.1186/s40104-024-01088-3Following 原文[1]发表后，作者报告原图3A中W14上DHE和DAPI荧光染色的图片以及合并后的图片放置顺序有误。原图3为：图3肝脏氧化还原参数。A 和 B 冷冻肝脏切片的 DHE 染色和 ROS 的荧光强度；C-E 肝脏 H2O2、O2- 和 T-AOC 含量；F-H 肝脏 T-SOD、CAT 和 GPX 活性；I 肝脏 MDA 含量。W0、W1、W4、W7 和 W14 分别代表 21、22、25、28 和 35 天龄。数据以平均值 ± SEM 表示（ROS，n = 3；其他，n = 6）。不同字母的数值差异显著（P < 0.05）。ROS：活性氧；T-AOC：总抗氧化能力；T-SOD：总超氧化物歧化酶；CAT：过氧化氢酶；GPX：谷胱甘肽过氧化物酶；MDA：丙二醛：全图正确的图 3 应该是：图 3 肝脏氧化还原参数。A 和 B 冷冻肝脏切片的 DHE 染色和 ROS 的荧光强度；C-E 肝脏 H2O2、O2- 和 T-AOC 含量；F-H 肝脏 T-SOD、CAT 和 GPX 活性；I 肝脏 MDA 含量。W0、W1、W4、W7 和 W14 分别代表 21、22、25、28 和 35 天龄。数据以平均值 ± SEM 表示（ROS，n = 3；其他，n = 6）。不同字母的数值差异显著（P < 0.05）。ROS：活性氧；T-AOC：总抗氧化能力；T-SOD：总超氧化物歧化酶；CAT：过氧化氢酶；GPX：谷胱甘肽过氧化物酶；MDA：丙二醛：Yu C, Luo Y, Shen C, et al. 微生物衍生抗氧化剂对断奶仔猪生长性能、肝脏氧化应激、线粒体功能和细胞凋亡的影响。J Anim Sci Biotechnol.2024;15:128. https://doi.org/10.1186/s40104-024-01088-3.Article PubMed PubMed Central Google Scholar Download references作者及单位上海交通大学农业与生物学院兽医生物技术上海市重点实验室，上海，200240Chengbing Yu，Yuxiao Luo，Cheng Shen，Zhen Luo，Hongcai Zhang，Jing Zhang，Weina Xu &；Jianxiong Xu作者：Chengbing Yu查看作者发表的论文您也可以在PubMed Google Scholar中搜索该作者Yuxiao Luo查看作者发表的论文您也可以在PubMed Google Scholar中搜索该作者Cheng Shen查看作者发表的论文您也可以在PubMed Google Scholar中搜索该作者Zhen Luo查看作者发表的论文您也可以在PubMed Google Scholar中搜索该作者Hongcai Zhang查看作者发表的论文您也可以在PubMed Google Scholar中搜索该作者Hongcai Zhang查看作者发表的论文您也可以在PubMed Google Scholar中搜索Hongcai Zhang查看作者发表的论文发表文章您也可以在 PubMed Google Scholar中搜索该作者张静发表文章您也可以在 PubMed Google Scholar中搜索该作者徐伟娜发表文章您也可以在 PubMed Google Scholar中搜索该作者徐建雄发表文章您也可以在 PubMed Google Scholar中搜索该作者通讯作者：徐建雄。开放存取 本文采用知识共享署名 4.0 国际许可协议进行许可，该协议允许以任何媒介或格式使用、共享、改编、分发和复制本文，但必须注明原作者和出处，提供知识共享许可协议的链接，并说明是否进行了修改。本文中的图片或其他第三方材料均包含在文章的知识共享许可协议中，除非在材料的署名栏中另有说明。如果材料未包含在文章的知识共享许可协议中，且您打算使用的材料不符合法律规定或超出许可使用范围，则您需要直接从版权所有者处获得许可。要查看该许可的副本，请访问 http://creativecommons.org/licenses/by/4.0/。除非在数据的信用行中另有说明，否则知识共享公共领域专用免责声明 (http://creativecommons.org/publicdomain/zero/1.0/) 适用于本文提供的数据。转载与许可引用本文Yu, C., Luo, Y., Shen, C. et al. Correction：微生物源抗氧化剂对断奶仔猪生长性能、肝脏氧化应激、线粒体功能和细胞凋亡的影响。J Animal Sci Biotechnol 15, 151 (2024). https://doi.org/10.1186/s40104-024-01116-2Download citationPublished: 04 November 2024DOI: https://doi.org/10.1186/s40104-024-01116-2Share this articleAnyone you share the following link with will be able to read this content:Get shareable linkSorry, a shareable link is not currently available for this article.Copy to clipboard Provided by the Springer Nature SharedIt content-sharing initiative
{"title":"Correction: Effects of microbe-derived antioxidants on growth performance, hepatic oxidative stress, mitochondrial function and cell apoptosis in weaning piglets","authors":"Chengbing Yu, Yuxiao Luo, Cheng Shen, Zhen Luo, Hongcai Zhang, Jing Zhang, Weina Xu, Jianxiong Xu","doi":"10.1186/s40104-024-01116-2","DOIUrl":"https://doi.org/10.1186/s40104-024-01116-2","url":null,"abstract":"<p><b>Correction</b><b>: </b><b>J Animal Sci Biotechnol 15, 128 (2024)</b></p><p><b>https://doi.org/10.1186/s40104-024-01088-3</b></p><br/><p>Following publication of the original article [1], the authors reported that in the original Fig. 3A, on W14, the pictures stained by DHE and DAPI fluorochrome, and the merged picture were placed out of order.</p><p>The original Fig. 3 was:</p><figure><figcaption><b data-test=\"figure-caption-text\">Fig. 3</b></figcaption><picture><source srcset=\"//media.springernature.com/lw685/springer-static/image/art%3A10.1186%2Fs40104-024-01116-2/MediaObjects/40104_2024_1116_Fig1_HTML.png?as=webp\" type=\"image/webp\"/><img alt=\"figure 1\" aria-describedby=\"Fig1\" height=\"465\" loading=\"lazy\" src=\"//media.springernature.com/lw685/springer-static/image/art%3A10.1186%2Fs40104-024-01116-2/MediaObjects/40104_2024_1116_Fig1_HTML.png\" width=\"685\"/></picture><p>The hepatic redox parameters. <b>A</b> and <b>B</b> DHE Staining with frozen liver sections and fluorescence intensity of ROS; <b>C</b>–<b>E</b> Hepatic H<sub>2</sub>O<sub>2</sub>, O<sub>2</sub><sup>−</sup> and T-AOC content; <b>F</b>–<b>H</b> Hepatic T-SOD, CAT and GPX activity; <b>I</b> Hepatic MDA content. W0, W1, W4, W7, and W14 respectively represented 21, 22, 25, 28, and 35 days of age. Data were presented as mean ± SEM (ROS, <i>n</i> = 3; others, <i>n</i> = 6). Values with different letters differ significantly (<i>P</i> < 0.05). ROS: Reactive oxygen species; T-AOC: Total antioxidant capacity; T-SOD: Total superoxide dismutase; CAT: Catalase; GPX: Glutathione peroxidase; MDA: Malonaldehyde</p><span>Full size image</span><svg aria-hidden=\"true\" focusable=\"false\" height=\"16\" role=\"img\" width=\"16\"><use xlink:href=\"#icon-eds-i-chevron-right-small\" xmlns:xlink=\"http://www.w3.org/1999/xlink\"></use></svg></figure><p>The correct Fig. 3 should be:</p><figure><figcaption><b data-test=\"figure-caption-text\">Fig. 3</b></figcaption><picture><source srcset=\"//media.springernature.com/lw685/springer-static/image/art%3A10.1186%2Fs40104-024-01116-2/MediaObjects/40104_2024_1116_Fig2_HTML.png?as=webp\" type=\"image/webp\"/><img alt=\"figure 2\" aria-describedby=\"Fig2\" height=\"494\" loading=\"lazy\" src=\"//media.springernature.com/lw685/springer-static/image/art%3A10.1186%2Fs40104-024-01116-2/MediaObjects/40104_2024_1116_Fig2_HTML.png\" width=\"685\"/></picture><p>The hepatic redox parameters. <b>A</b> and <b>B</b> DHE Staining with frozen liver sections and fluorescence intensity of ROS; <b>C</b>–<b>E</b> Hepatic H<sub>2</sub>O<sub>2</sub>, O<sub>2</sub><sup>−</sup> and T-AOC content; <b>F</b>–<b>H</b> Hepatic T-SOD, CAT and GPX activity; <b>I</b> Hepatic MDA content. W0, W1, W4, W7, and W14 respectively represented 21, 22, 25, 28, and 35 days of age. Data were presented as mean ± SEM (ROS, <i>n</i> = 3; others, <i>n</i> = 6). Values with different letters differ significantly (<i>P</i> < 0.05). ROS: Reactive oxygen species; T-AOC: Total antioxidant capacity; T-SOD: Total superoxide dismutase; ","PeriodicalId":14928,"journal":{"name":"Journal of Animal Science and Biotechnology","volume":"17 1","pages":""},"PeriodicalIF":7.0,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142574490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-02DOI: 10.1186/s40104-024-01105-5
Hemlata Gautam, Noor Ahmad Shaik, Babajan Banaganapalli, Shelly Popowich, Iresha Subhasinghe, Lisanework E. Ayalew, Rupasri Mandal, David S. Wishart, Suresh Tikoo, Susantha Gomis
Necrotic enteritis (NE) is an economically important disease of broiler chickens caused by Clostridium perfringens (CP). The pathogenesis, or disease process, of NE is still not clear. This study aimed to identify the alterations of metabolites and metabolic pathways associated with subclinical or clinical NE in CP infected birds and to investigate the possible variations in the metabolic profile of birds infected with different isolates of CP. Using a well-established NE model, the protein content of feed was changed abruptly before exposing birds to CP isolates with different toxin genes combinations (cpa, cpb2, netB, tpeL; cpa, cpb2, netB; or cpa, cpb2). Metabolomics analysis of jejunal contents was performed by a targeted, fully quantitative LC-MS/MS based assay. This study detected statistically significant differential expression of 34 metabolites including organic acids, amino acids, fatty acids, and biogenic amines, including elevation of butyric acid at onset of NE in broiler chickens. Subsequent analysis of broilers infected with CP isolates with different toxin gene combinations confirmed an elevation of butyric acid consistently among 21 differentially expressed metabolites including organic acids, amino acids, and biogenic amines, underscoring its potential role during the development of NE. Furthermore, protein-metabolite network analysis revealed significant alterations in butyric acid and arginine-proline metabolisms. This study indicates a significant metabolic difference between CP-infected and non-infected broiler chickens. Among all the metabolites, butyric acid increased significantly in CP-infected birds compared to non-infected healthy broilers. Logistic regression analysis revealed a positive association between butyric acid (coefficient: 1.23, P < 0.01) and CP infection, while showing a negative association with amino acid metabolism. These findings suggest that butyric acid could be a crucial metabolite linked to the occurrence of NE in broiler chickens and may serve as an early indicator of the disease at the farm level. Further metabolomic experiments using different NE animal models and field studies are needed to determine the specificity and to validate metabolites associated with NE, regardless of predisposing factors.
坏死性肠炎（NE）是由产气荚膜梭菌（CP）引起的肉鸡的一种重要经济疾病。坏死性肠炎的发病机制或疾病过程尚不清楚。本研究旨在确定受 CP 感染的禽类体内与亚临床或临床 NE 相关的代谢物和代谢途径的变化，并调查受不同 CP 分离物感染的禽类在代谢方面可能存在的变化。利用一个成熟的NE模型，在鸟类接触具有不同毒素基因组合（cpa、cpb2、netB、tpeL；cpa、cpb2、netB；或cpa、cpb2）的CP分离物之前，突然改变饲料中的蛋白质含量。空肠内容物的代谢组学分析是通过一种基于靶向、完全定量的 LC-MS/MS 方法进行的。该研究检测到 34 种代谢物（包括有机酸、氨基酸、脂肪酸和生物胺）存在统计学意义上的差异表达，其中包括肉鸡 NE 发病时丁酸的升高。随后对感染了具有不同毒素基因组合的 CP 分离物的肉鸡进行的分析证实，在包括有机酸、氨基酸和生物胺在内的 21 种差异表达代谢物中，丁酸的表达量一直在升高，这突显了丁酸在 NE 发生过程中的潜在作用。此外，蛋白质代谢物网络分析显示，丁酸和精氨酸-脯氨酸代谢发生了显著变化。这项研究表明，感染氯化石蜡的肉鸡与未感染氯化石蜡的肉鸡在代谢方面存在明显差异。在所有代谢物中，与未感染 CP 的健康肉鸡相比，感染 CP 的肉鸡体内的丁酸明显增加。逻辑回归分析表明，丁酸（系数：1.23，P < 0.01）与CP感染呈正相关，而与氨基酸代谢呈负相关。这些研究结果表明，丁酸可能是与肉鸡NE发生相关的重要代谢物，可作为鸡场的早期疾病指标。需要利用不同的 NE 动物模型和现场研究开展进一步的代谢组学实验，以确定特异性并验证与 NE 相关的代谢物，而不考虑易感因素。
{"title":"Elevated levels of butyric acid in the jejunum of an animal model of broiler chickens: from early onset of Clostridium perfringens infection to clinical disease of necrotic enteritis","authors":"Hemlata Gautam, Noor Ahmad Shaik, Babajan Banaganapalli, Shelly Popowich, Iresha Subhasinghe, Lisanework E. Ayalew, Rupasri Mandal, David S. Wishart, Suresh Tikoo, Susantha Gomis","doi":"10.1186/s40104-024-01105-5","DOIUrl":"https://doi.org/10.1186/s40104-024-01105-5","url":null,"abstract":"Necrotic enteritis (NE) is an economically important disease of broiler chickens caused by Clostridium perfringens (CP). The pathogenesis, or disease process, of NE is still not clear. This study aimed to identify the alterations of metabolites and metabolic pathways associated with subclinical or clinical NE in CP infected birds and to investigate the possible variations in the metabolic profile of birds infected with different isolates of CP. Using a well-established NE model, the protein content of feed was changed abruptly before exposing birds to CP isolates with different toxin genes combinations (cpa, cpb2, netB, tpeL; cpa, cpb2, netB; or cpa, cpb2). Metabolomics analysis of jejunal contents was performed by a targeted, fully quantitative LC-MS/MS based assay. This study detected statistically significant differential expression of 34 metabolites including organic acids, amino acids, fatty acids, and biogenic amines, including elevation of butyric acid at onset of NE in broiler chickens. Subsequent analysis of broilers infected with CP isolates with different toxin gene combinations confirmed an elevation of butyric acid consistently among 21 differentially expressed metabolites including organic acids, amino acids, and biogenic amines, underscoring its potential role during the development of NE. Furthermore, protein-metabolite network analysis revealed significant alterations in butyric acid and arginine-proline metabolisms. This study indicates a significant metabolic difference between CP-infected and non-infected broiler chickens. Among all the metabolites, butyric acid increased significantly in CP-infected birds compared to non-infected healthy broilers. Logistic regression analysis revealed a positive association between butyric acid (coefficient: 1.23, P < 0.01) and CP infection, while showing a negative association with amino acid metabolism. These findings suggest that butyric acid could be a crucial metabolite linked to the occurrence of NE in broiler chickens and may serve as an early indicator of the disease at the farm level. Further metabolomic experiments using different NE animal models and field studies are needed to determine the specificity and to validate metabolites associated with NE, regardless of predisposing factors.","PeriodicalId":14928,"journal":{"name":"Journal of Animal Science and Biotechnology","volume":"141 1","pages":""},"PeriodicalIF":7.0,"publicationDate":"2024-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142563093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Saline-alkaline water aquaculture has become a key way to mitigate the reduction of freshwater aquaculture space and meet the increasing global demand for aquatic products. To enhance the comprehensive utilization capability of saline-alkaline water, it is necessary to understand the regulatory mechanisms of aquatic animals coping with saline-alkaline water. In this study, our objective was to elucidate the function of proline metabolism in the alkaline adaptation of Nile tilapia (Oreochromis niloticus). Expose Nile tilapia to alkaline water of different alkalinity for 2 weeks to observe changes in its growth performance and proline metabolism. Meanwhile, to further clarify the role of proline metabolism, RNA interference experiments were conducted to disrupt the normal operation of proline metabolic axis by knocking down pycr (pyrroline-5-carboxylate reductases), the final rate-limiting enzyme in proline synthesis. The results showed that both the synthesis and degradation of proline were enhanced under carbonate alkalinity stress, and the environmental alkalinity impaired the growth performance of tilapia, and the higher the alkalinity, the greater the impairment. Moreover, environmental alkalinity caused oxidative stress in tilapia, enhanced ion transport, ammonia metabolism, and altered the intensity and form of energy metabolism in tilapia. When the expression level of the pycr gene decreased, the proline metabolism could not operate normally, and the ion transport, antioxidant defense system, and energy metabolism were severely damaged, ultimately leading to liver damage and a decreased survival rate of tilapia under alkalinity stress. The results indicated that proline metabolism plays an important role in the alkaline adaptation of Nile tilapia and is a key regulatory process in various biochemical and physiological processes.
{"title":"Proline metabolism is essential for alkaline adaptation of Nile tilapia (Oreochromis niloticus)","authors":"Minxu Wang, Yuxi Yan, Wei Liu, Jinquan Fan, Erchao Li, Liqiao Chen, Xiaodan Wang","doi":"10.1186/s40104-024-01100-w","DOIUrl":"https://doi.org/10.1186/s40104-024-01100-w","url":null,"abstract":"Saline-alkaline water aquaculture has become a key way to mitigate the reduction of freshwater aquaculture space and meet the increasing global demand for aquatic products. To enhance the comprehensive utilization capability of saline-alkaline water, it is necessary to understand the regulatory mechanisms of aquatic animals coping with saline-alkaline water. In this study, our objective was to elucidate the function of proline metabolism in the alkaline adaptation of Nile tilapia (Oreochromis niloticus). Expose Nile tilapia to alkaline water of different alkalinity for 2 weeks to observe changes in its growth performance and proline metabolism. Meanwhile, to further clarify the role of proline metabolism, RNA interference experiments were conducted to disrupt the normal operation of proline metabolic axis by knocking down pycr (pyrroline-5-carboxylate reductases), the final rate-limiting enzyme in proline synthesis. The results showed that both the synthesis and degradation of proline were enhanced under carbonate alkalinity stress, and the environmental alkalinity impaired the growth performance of tilapia, and the higher the alkalinity, the greater the impairment. Moreover, environmental alkalinity caused oxidative stress in tilapia, enhanced ion transport, ammonia metabolism, and altered the intensity and form of energy metabolism in tilapia. When the expression level of the pycr gene decreased, the proline metabolism could not operate normally, and the ion transport, antioxidant defense system, and energy metabolism were severely damaged, ultimately leading to liver damage and a decreased survival rate of tilapia under alkalinity stress. The results indicated that proline metabolism plays an important role in the alkaline adaptation of Nile tilapia and is a key regulatory process in various biochemical and physiological processes.","PeriodicalId":14928,"journal":{"name":"Journal of Animal Science and Biotechnology","volume":"229 1","pages":""},"PeriodicalIF":7.0,"publicationDate":"2024-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142431690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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