将玉米种子置于液氮中会改变幼苗的形态和激素反应。

IF 1 4区 生物学 Q3 BIOLOGY Cryo letters Pub Date : 2023-11-01
J C Lorenzo, Y Acosta, B E Zevallos-Bravo, P Chmielarz, E Hajari, M Hofer, J Ludwig-Mueller
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引用次数: 0

摘要

背景:低温保存是目前长期保存植物种质的最合适策略。虽然很多工作都集中在开发成功低温贮藏的方案上,但考虑到低温对种子和发育中幼苗的植物激素状态的影响的报道却很少:研究低温贮藏对玉米种子外部种皮特征以及吲哚-3-乙酸(IAA)、脱落酸(ABA)和 1-氨基环丙烷-1-羧酸(ACC)水平的影响:两组含水量为 6% 的种子进行了比较:一组保持在 4 摄氏度(对照组),另一组暴露在低温下的 LN 中:结果:与对照组种子相比,暴露在低温下的种子种皮出现大面积裂纹。低温还导致生物量和植株高度下降。植物激素分析结果显示,生长 7 天后,IAA、ABA 和 ACC 的水平开始下降,14 天后,相对于对照的水平急剧上升。虽然人们对 ABA 和乙烯(以及其前体 ACC)作为胁迫响应分子的作用进行了深入研究,但对辅助素在低温胁迫条件下调节植物生长的潜在重要作用却知之甚少:据推测,这三种激素的相互作用会调节各种胁迫反应和恢复途径之间的相互影响,从而减轻冷冻胁迫造成的损害,使植物得以存活。鉴于低温生物学中有关植物激素的信息十分匮乏,需要进行更多的研究,以充分阐明液氮造成的冷冻胁迫中的这些关系。Doi.org/10.54680/fr23610110612.
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Exposure of maize seeds to liquid nitrogen modifies the morphology and hormonal response of young plants.

Background: Cryopreservation currently represents the most suitable strategy for the long-term conservation of plant germplasm. While much effort has focused on the development of protocols to enable successful cryostorage, there are few, if any reports, that consider the effect of cryogenic temperatures on the phytohormone status of the seed and developing seedlings.

Objective: To investigate the effect of cryopreservation on external seed coat features as well as levels of indole-3-acetic acid (IAA), abscisic acid (ABA) and 1-aminocyclopropane-1-carboxylic acid (ACC) in maize.

Materials and methods: Two groups of seeds at 6% moisture content were compared: one was maintained at 4 degree C (control) while the other was exposed to LN within cryo-vials.

Results: Seeds exposed to cryogenic temperatures were characterized by the presence of large cracks in the seed coat compared with control seeds. Cryogenic exposure also resulted in a reduction in biomass and plant height. Results from the phytohormone analysis showed an initial reduction in the levels of IAA, ABA and ACC after 7 days of growth followed by sharp increase in levels relative to the control by 14 days. Whilst the roles of ABA and ethylene (and by extension, its precursor ACC) are well studied as stress response molecules, much less is known about the potentially vital role of auxins in regulating plant growth under conditions of low temperature stress.

Conclusion: It is postulated that the interaction of all three hormones modulate crosstalk between various stress responses and recovery pathways to ameliorate the damage caused by freezing stress and enable plant survival. Given the dearth of information on phytohormones in cryobiology, more studies are needed to fully elucidate these relationships in the context of freezing stress caused by liquid nitrogen. Doi.org/10.54680/fr23610110612.

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来源期刊
Cryo letters
Cryo letters 生物-生理学
CiteScore
1.80
自引率
10.00%
发文量
50
审稿时长
1 months
期刊介绍: A bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.
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